##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064080_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1031634
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.212918535061853	32.0	32.0	32.0	32.0	32.0
2	31.41236620739526	32.0	32.0	32.0	32.0	32.0
3	31.49155902190118	32.0	32.0	32.0	32.0	32.0
4	31.562802311672552	32.0	32.0	32.0	32.0	32.0
5	31.51269733258113	32.0	32.0	32.0	32.0	32.0
6	35.087820874457414	36.0	36.0	36.0	36.0	36.0
7	35.112642662029366	36.0	36.0	36.0	36.0	36.0
8	35.06373772093591	36.0	36.0	36.0	36.0	36.0
9	35.160424142670756	36.0	36.0	36.0	36.0	36.0
10	35.024979789343895	36.0	36.0	36.0	36.0	36.0
11	35.16717750675143	36.0	36.0	36.0	36.0	36.0
12	35.069109781182085	36.0	36.0	36.0	36.0	36.0
13	35.100359235930576	36.0	36.0	36.0	36.0	36.0
14	35.049588322990516	36.0	36.0	36.0	36.0	36.0
15	35.02846261367888	36.0	36.0	36.0	36.0	36.0
16	35.03167208525504	36.0	36.0	36.0	36.0	36.0
17	34.99923034719678	36.0	36.0	36.0	36.0	36.0
18	34.985877743463284	36.0	36.0	36.0	36.0	36.0
19	34.97891500280138	36.0	36.0	36.0	36.0	36.0
20	34.96874763724344	36.0	36.0	36.0	36.0	36.0
21	34.94846718894492	36.0	36.0	36.0	36.0	36.0
22	34.945310061514064	36.0	36.0	36.0	36.0	36.0
23	34.891727104767774	36.0	36.0	36.0	32.0	36.0
24	34.855703670100056	36.0	36.0	36.0	32.0	36.0
25	34.828675673737	36.0	36.0	36.0	32.0	36.0
26	34.768666988486224	36.0	36.0	36.0	32.0	36.0
27	34.75750799217552	36.0	36.0	36.0	32.0	36.0
28	34.7309617558165	36.0	36.0	36.0	32.0	36.0
29	34.684074972325455	36.0	36.0	36.0	32.0	36.0
30	34.666017211530445	36.0	36.0	36.0	32.0	36.0
31	34.63397968659428	36.0	36.0	36.0	32.0	36.0
32	34.577906505601796	36.0	36.0	36.0	32.0	36.0
33	34.54638079008641	36.0	36.0	36.0	32.0	36.0
34	34.51668324231268	36.0	36.0	36.0	32.0	36.0
35	34.4495635079883	36.0	36.0	36.0	32.0	36.0
36	34.408621662333736	36.0	36.0	36.0	32.0	36.0
37	34.37430910574874	36.0	36.0	36.0	32.0	36.0
38	34.315730191133675	36.0	36.0	36.0	32.0	36.0
39	34.282669047355945	36.0	36.0	36.0	32.0	36.0
40	34.23135627557836	36.0	36.0	36.0	32.0	36.0
41	34.2055331638934	36.0	36.0	36.0	32.0	36.0
42	34.113251405052566	36.0	36.0	36.0	32.0	36.0
43	34.077068029940854	36.0	36.0	36.0	32.0	36.0
44	34.01784353753366	36.0	36.0	36.0	32.0	36.0
45	33.92132965761113	36.0	36.0	36.0	32.0	36.0
46	33.885986696832404	36.0	36.0	36.0	32.0	36.0
47	33.80464583369683	36.0	36.0	36.0	32.0	36.0
48	33.74361934562064	36.0	36.0	36.0	27.0	36.0
49	33.70767830451497	36.0	36.0	36.0	27.0	36.0
50	33.127065412733586	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	7.0
21	49.0
22	145.0
23	425.0
24	1072.0
25	2527.0
26	5156.0
27	9297.0
28	15284.0
29	22015.0
30	29998.0
31	41259.0
32	57460.0
33	87771.0
34	172993.0
35	586171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.247106268297884	17.64061500281133	11.710452333404424	25.40182639548636
2	15.777377332354945	20.26765436023997	37.67943708445575	26.275531222949333
3	18.400316392811757	25.333622843095725	28.65952779536054	27.60653296873198
4	11.923511633001628	15.64188462187171	36.083630434824755	36.35097331030191
5	13.49984587557215	37.93428677224674	33.22147195613948	15.344395396041618
6	33.25449385052034	36.31546908199823	16.840695131597315	13.589341935884114
7	29.797874052231705	31.094167117407917	20.639587295494334	18.468371534866048
8	28.9334201858411	32.67311856724381	18.85891701902031	19.534544227894777
9	28.18126741790864	13.299600145401671	18.13604749787956	40.38308493881013
10	15.790810367005648	26.73971689160436	31.466752744957198	26.002719996432795
11	37.521482930460735	20.772212598860254	22.110576144811187	19.595728325867825
12	24.924779378693177	23.820509639096112	29.382667462816197	21.872043519394513
13	30.302231329734486	19.77032396399086	25.55332499052468	24.374119715749977
14	23.891659041208495	19.671646478589263	25.65323681312716	30.78345766707508
15	25.65577997588203	26.926723252125832	23.164009941876472	24.253486830115666
16	25.409959219756757	25.7144297309008	24.564886339733256	24.31072470960919
17	23.159667091235843	25.794904006653525	26.032197465380165	25.01323143673047
18	24.078667663146998	25.124002671497852	27.52767220062212	23.26965746473303
19	26.101408057508767	24.810349406863285	25.89067440584548	23.19756812978246
20	25.930858797938793	24.149208823335545	26.418922324713947	23.50101005401172
21	26.57027589241921	23.25960563533191	25.592506644798448	24.577611827450433
22	25.633074584735642	24.31787691735037	25.869883834545654	24.179164663368333
23	24.28377420821366	24.30820173648707	26.340533314980373	25.067490740318892
24	25.1803456665665	25.03038880218683	25.941083528009074	23.848182003237596
25	24.779835073811594	24.53100145112557	25.886936283276775	24.802227191786066
26	24.15146577120572	24.898674160723477	26.730052066116507	24.219808001954295
27	24.55955314541193	25.250638349848675	26.115730840295242	24.074077664444154
28	24.299309124079453	24.586148165596956	26.44851777209722	24.666024938226368
29	24.27274154868003	24.86356330953244	26.235878923705958	24.62781621808157
30	24.25239121394276	25.120663364964145	26.706372402187306	23.920573018905785
31	24.562260675682225	24.757500848238088	25.327710726576512	25.352527749503174
32	23.77852246851732	25.283176893347125	25.642231138262055	25.296069499873497
33	24.101114500723643	24.72733653036159	26.137499357783135	25.034049611131632
34	24.460768490326256	25.16849924438279	26.4470869236056	23.92364534168535
35	25.04926798390466	24.605395680315002	26.220067215779714	24.12526912000062
36	24.332302215949134	25.23209835134315	25.85803927423037	24.577560158477347
37	25.183792880158514	25.309035707777078	25.16333913663876	24.34383227542565
38	24.62682226483356	25.451409825640347	25.712183460553383	24.209584448972706
39	25.095798625618098	24.646201837743835	25.752260343488842	24.505739193149225
40	25.680856529388674	25.29902433586499	25.381032468822855	23.63908666592349
41	24.279345519616673	25.253757038843656	26.322876084362402	24.14402135717727
42	25.202547474336235	26.18906369655199	25.472804645166292	23.135584183945486
43	24.22737873902937	25.20962216634452	25.60046451302711	24.962534581599
44	24.037025225349232	25.765373136064508	25.43850890976434	24.759092728821923
45	24.56490846453449	25.5620760317248	25.134787742021786	24.738227761718925
46	24.429977530616714	25.308978253747	25.154852630701434	25.10619158493485
47	24.82575914728126	25.28445606804069	25.2042907355242	24.685494049153853
48	25.003416972417035	25.948052325722287	24.23762740945023	24.810903292410444
49	24.93297015402809	25.65600069792464	25.304808894661846	24.10622025338542
50	24.305948678976062	26.459349530449078	24.26116492055245	24.97353687002241
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	33.5
2	26.0
3	95.0
4	164.0
5	195.0
6	226.0
7	273.0
8	320.0
9	462.0
10	604.0
11	872.0
12	1140.0
13	2126.5
14	3113.0
15	4423.0
16	5733.0
17	6368.0
18	7003.0
19	7156.0
20	7309.0
21	7459.0
22	7609.0
23	7887.0
24	8165.0
25	7697.5
26	7230.0
27	8755.0
28	10280.0
29	12080.5
30	13881.0
31	15955.0
32	18029.0
33	21498.5
34	24968.0
35	28411.0
36	31854.0
37	35408.0
38	38962.0
39	40697.5
40	42433.0
41	46370.0
42	50307.0
43	51918.5
44	53530.0
45	60193.5
46	66857.0
47	69630.5
48	72404.0
49	75452.5
50	78501.0
51	74951.5
52	71402.0
53	69740.0
54	68078.0
55	67683.5
56	67289.0
57	65586.0
58	63883.0
59	58877.0
60	53871.0
61	48148.5
62	42426.0
63	36747.0
64	31068.0
65	26371.5
66	21675.0
67	18222.5
68	14770.0
69	13866.0
70	12962.0
71	10305.5
72	7649.0
73	7385.0
74	7121.0
75	6091.0
76	5061.0
77	5123.0
78	5185.0
79	4659.5
80	4134.0
81	3057.0
82	1980.0
83	1633.5
84	1287.0
85	964.5
86	642.0
87	443.0
88	244.0
89	169.5
90	95.0
91	77.0
92	59.0
93	43.0
94	27.0
95	23.0
96	19.0
97	14.5
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009111758627575284
2	4.846680121050683E-4
3	4.846680121050683E-4
4	0.0
5	0.0
6	1.938672048420273E-4
7	0.0
8	0.0
9	8.724024217891228E-4
10	0.0014540040363152048
11	9.693360242101366E-5
12	1.938672048420273E-4
13	6.785352169470956E-4
14	1.938672048420273E-4
15	0.0036834768919985186
16	6.785352169470956E-4
17	0.0
18	2.90800807263041E-4
19	0.0
20	5.81601614526082E-4
21	0.0
22	1.938672048420273E-4
23	0.00106626962663115
24	3.877344096840546E-4
25	0.002035605650841287
26	0.005912949747681833
27	0.006591484964628929
28	0.004362012108945614
29	0.0019386720484202732
30	0.004362012108945614
31	0.008142422603365148
32	0.003392676084735478
33	0.004943613723471696
34	0.0026172072653673686
35	0.0030049416750514233
36	0.006494551362207915
37	0.0036834768919985186
38	0.0085301570130492
39	0.0041681449041035875
40	0.002811074470209396
41	0.003586543289577505
42	0.002326406458104328
43	0.0015509376387362185
44	0.0012601368314731774
45	0.001163203229052164
46	7.754688193681092E-4
47	0.0015509376387362185
48	0.0018417384459992593
49	3.877344096840546E-4
50	9.693360242101366E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1031634.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.489890669317155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.15912361868354	40.20253305165076
2	14.287724607503954	15.284976543373977
3	4.849985760391066	7.782756242131892
4	2.081431447939158	4.4534216234377615
5	1.0640717755238747	2.8458541468539122
6	0.6400985789716029	2.054328180406579
7	0.3841513606154254	1.4383749995851973
8	0.246291537602112	1.053928593529199
9	0.19334591384281913	0.9307846613530448
>10	0.9041835725341085	9.023253581681445
>50	0.09909273608140476	3.7263936429718947
>100	0.08429552404701812	8.683843631647516
>500	0.005291277107451569	1.8728767289828276
>1k	9.12289156457167E-4	0.6466743723939665
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1565	0.15170108778888636	No Hit
GCCTAGTACCCTGGAAACGGTTCGTCCGGAGATAGGGTCGGACGGCTGGA	1442	0.1397782546911017	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1369	0.1327021017143677	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1189	0.11525405327858523	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1061	0.10284655216869548	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.938672048420273E-4	0.0	0.0	0.0
3	0.0	1.938672048420273E-4	0.0	0.0	0.0
4	0.0	1.938672048420273E-4	0.0	0.0	0.0
5	0.0	1.938672048420273E-4	0.0	0.0	0.0
6	0.0	1.938672048420273E-4	0.0	0.0	0.0
7	0.0	2.9080080726304094E-4	0.0	0.0	0.0
8	0.0	2.9080080726304094E-4	0.0	0.0	0.0
9	0.0	2.9080080726304094E-4	0.0	0.0	0.0
10	0.0	2.9080080726304094E-4	0.0	0.0	0.0
11	0.0	2.9080080726304094E-4	0.0	0.0	0.0
12	0.0	2.9080080726304094E-4	0.0	0.0	3.877344096840546E-4
13	0.0	2.9080080726304094E-4	0.0	9.693360242101366E-5	4.8466801210506825E-4
14	0.0	3.877344096840546E-4	0.0	2.9080080726304094E-4	4.8466801210506825E-4
15	0.0	3.877344096840546E-4	0.0	2.9080080726304094E-4	5.816016145260819E-4
16	0.0	4.8466801210506825E-4	0.0	3.877344096840546E-4	5.816016145260819E-4
17	0.0	7.754688193681092E-4	0.0	3.877344096840546E-4	5.816016145260819E-4
18	0.0	7.754688193681092E-4	0.0	3.877344096840546E-4	5.816016145260819E-4
19	0.0	7.754688193681092E-4	0.0	4.8466801210506825E-4	5.816016145260819E-4
20	0.0	7.754688193681092E-4	0.0	5.816016145260819E-4	5.816016145260819E-4
21	0.0	7.754688193681092E-4	0.0	7.754688193681092E-4	5.816016145260819E-4
22	0.0	8.724024217891229E-4	0.0	0.0014540040363152048	5.816016145260819E-4
23	0.0	9.693360242101365E-4	0.0	0.002811074470209396	5.816016145260819E-4
24	0.0	0.0010662696266311503	0.0	0.004943613723471696	5.816016145260819E-4
25	0.0	0.0010662696266311503	0.0	0.006203750554944874	5.816016145260819E-4
26	0.0	0.0010662696266311503	0.0	0.0072700201815760245	5.816016145260819E-4
27	0.0	0.0010662696266311503	0.0	0.00930562583241731	5.816016145260819E-4
28	0.0	0.0010662696266311503	0.0	0.012310567507468733	5.816016145260819E-4
29	0.0	0.0010662696266311503	0.0	0.02045299011083388	5.816016145260819E-4
30	0.0	0.0010662696266311503	0.0	0.034314495257038835	5.816016145260819E-4
31	0.0	0.0010662696266311503	0.0	0.06620565045355233	5.816016145260819E-4
32	0.0	0.0010662696266311503	0.0	0.09858147366217089	5.816016145260819E-4
33	0.0	0.0010662696266311503	0.0	0.1285339568102641	6.785352169470956E-4
34	0.0	0.0010662696266311503	0.0	0.16090978001888268	8.724024217891229E-4
35	0.0	0.0010662696266311503	0.0	0.19968322098728813	8.724024217891229E-4
36	0.0	0.0010662696266311503	0.0	0.2500886942462152	8.724024217891229E-4
37	0.0	0.0010662696266311503	0.0	0.3309313186653406	8.724024217891229E-4
38	0.0	0.0011632032290521638	0.0	0.43406867164129914	8.724024217891229E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	140	0.0	26.713669	10
TACGTCC	75	2.0659354E-6	23.46385	6
TAGGCTG	355	0.0	21.687536	5
ACTAGGC	325	0.0	21.657888	3
ACCGTCG	175	0.0	21.368862	8
ATACCGT	175	0.0	21.368862	6
GTCGTAG	200	0.0	20.898502	11
GATATAC	300	0.0	20.533854	1
TTATTCG	75	5.470569E-5	20.533854	22
AACGGTT	355	0.0	20.450232	16
ACGTATG	65	4.9258117E-4	20.311161	32
ACGTTGC	65	4.9322576E-4	20.307222	15
GTACTAG	360	0.0	20.167177	1
CGGTTTA	55	0.0044787447	20.003416	31
GCCTAGT	385	0.0	20.000505	1
CCTAGTA	435	0.0	19.21515	2
AAACGGT	390	0.0	19.179043	15
CGGTTCG	405	0.0	19.010984	18
ACTGTTC	375	0.0	18.77108	8
CCGTCGT	190	1.8189894E-12	18.524988	9
>>END_MODULE