FastQCFastQC Report
Thu 2 Feb 2017
SRR4064079_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064079_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492581
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT101970.4090940274358185No Hit
TATCAACGCAGAGTACTTTTTTTTT71970.28873685549235917No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52510.21066516995836845No Hit
GTACATGGGGTGGTATCAACGCAAA37830.15177039382070232No Hit
GGTATCAACGCAGAGTACTTTTTTT35900.14402741575900643No Hit
GAGTACTTTTTTTTTTTTTTTTTTT35580.14274360592494284No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34450.1382101524484059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1700.016.2030741
CGTTATT1800.013.7165412
CGAACGA1501.4551915E-1112.03463116
CGCGCTA802.8648241E-511.8762816
GCACCGT1157.0851456E-811.5646076
GATATAC4300.011.4863821
ACCGTCT752.07347E-411.4007718
ATACCGT4500.011.3993986
ACCGTCG4100.011.3544258
ACTCTAA4100.011.12292710
CGTCGTA4200.011.08430610
ACGGTAT3350.011.0606729
GTAGGAC13200.011.0079093
AAGACGG4500.010.9765375
TATACTG2600.010.9602995
TGTAGGA14100.010.9103752
TACGACA700.00149435710.8550444
GTTATTC2450.010.8537373
TTTTCGG3100.010.72696316
CGCATCG3550.010.70516413
Produced by FastQC (version 0.11.5)