##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064079_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2492581
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26403836023784	32.0	32.0	32.0	32.0	32.0
2	30.885139941289772	32.0	32.0	32.0	32.0	32.0
3	30.91210436090141	32.0	32.0	32.0	32.0	32.0
4	30.93481295091313	32.0	32.0	32.0	32.0	32.0
5	30.844626112451312	32.0	32.0	32.0	32.0	32.0
6	34.509975804196536	36.0	36.0	36.0	32.0	36.0
7	34.43701408299269	36.0	36.0	36.0	32.0	36.0
8	34.39599154450748	36.0	36.0	36.0	32.0	36.0
9	34.52222696072866	36.0	36.0	36.0	32.0	36.0
10	34.23104404631184	36.0	36.0	36.0	32.0	36.0
11	34.52512355666676	36.0	36.0	36.0	32.0	36.0
12	34.33942367369406	36.0	36.0	36.0	32.0	36.0
13	34.42027801704338	36.0	36.0	36.0	32.0	36.0
14	34.32050312507397	36.0	36.0	36.0	32.0	36.0
15	34.26463814014469	36.0	36.0	36.0	32.0	36.0
16	34.25651724056309	36.0	36.0	36.0	32.0	36.0
17	34.18240169527088	36.0	36.0	36.0	32.0	36.0
18	34.186004386617725	36.0	36.0	36.0	32.0	36.0
19	34.186118725931074	36.0	36.0	36.0	32.0	36.0
20	34.16537637091834	36.0	36.0	36.0	32.0	36.0
21	34.1422826379564	36.0	36.0	36.0	32.0	36.0
22	34.12879180255326	36.0	36.0	36.0	32.0	36.0
23	34.07924516796044	36.0	36.0	36.0	32.0	36.0
24	34.06239837341294	36.0	36.0	36.0	32.0	36.0
25	33.671435351549256	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	24.0
5	28.0
6	95.0
7	41.0
8	149.0
9	199.0
10	238.0
11	96.0
12	158.0
13	121.0
14	251.0
15	443.0
16	731.0
17	938.0
18	1223.0
19	1611.0
20	2448.0
21	3543.0
22	5617.0
23	8364.0
24	12609.0
25	18236.0
26	25980.0
27	34817.0
28	48552.0
29	64786.0
30	86341.0
31	118518.0
32	167197.0
33	239345.0
34	516529.0
35	1133349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.82979985495514	17.08615560094283	11.443693980237137	25.64035056386489
2	16.20552122748152	19.80217881741401	38.62472030565872	25.367579649445748
3	18.976989382338132	24.53901534375508	28.37028017548329	28.113715098423498
4	12.403164221524394	15.515269250505417	35.99432375537263	36.087242772597556
5	14.200430596937675	37.05922192433338	33.59988187873793	15.140465599991012
6	33.48290360802343	35.66964162116897	17.3716852756805	13.475769495127096
7	29.84964542194628	30.49499674154165	20.949038032469012	18.706319804043055
8	27.775405413432203	33.378671356456394	19.457155768818623	19.38876746129278
9	27.78055633604945	14.041223457632562	18.581142375466627	39.59707783085136
10	15.951296802322199	26.871821922058984	31.74491156277435	25.431969712844467
11	36.62701287961961	21.155573039706642	23.161959661119685	19.05545441955406
12	25.314567854844544	23.49197517259431	29.3155200993032	21.87793687325795
13	29.903851558046362	19.86571334516287	25.282985539595494	24.947449557195274
14	23.53361534981137	20.01761632778253	25.69290019434969	30.755868128056413
15	24.530686290134486	27.95047512455686	22.78261490924842	24.736223676060234
16	24.761268151288508	25.860924094794193	24.915270568114757	24.462537185802553
17	23.240034267555096	26.207132436713813	25.842497709742247	24.71033558598884
18	23.92587762653585	25.16086239750618	27.445658501958132	23.467601473999835
19	25.459181645501772	24.639606597553037	25.918172580109616	23.98303917683557
20	25.784337265224806	24.441233225754385	25.6648065867547	24.109622922266112
21	26.518333950962518	24.087738943199664	24.868641714982935	24.525285390854886
22	25.732050753912905	24.677648732878666	25.375912528918665	24.21438798428977
23	24.098516526360505	24.747300347800806	26.13107537183207	25.023107754006617
24	24.675604164408202	24.997811798621232	26.106005307893987	24.22057872907658
25	24.4718246946623	24.904772057208636	26.20823150056432	24.415171747564745
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	343.0
1	343.0
2	900.5
3	1458.0
4	1458.0
5	1458.0
6	4058.5
7	6659.0
8	6659.0
9	6659.0
10	6639.0
11	6619.0
12	6619.0
13	6619.0
14	6599.0
15	6579.0
16	6579.0
17	6579.0
18	11655.0
19	16731.0
20	16731.0
21	16731.0
22	27703.0
23	38675.0
24	38675.0
25	38675.0
26	59377.0
27	80079.0
28	80079.0
29	80079.0
30	102489.0
31	124899.0
32	124899.0
33	124899.0
34	145842.5
35	166786.0
36	166786.0
37	166786.0
38	186738.0
39	206690.0
40	206690.0
41	206690.0
42	234878.0
43	263066.0
44	263066.0
45	263066.0
46	293572.0
47	324078.0
48	324078.0
49	324078.0
50	328870.0
51	333662.0
52	333662.0
53	333662.0
54	306765.5
55	279869.0
56	279869.0
57	279869.0
58	256625.0
59	233381.0
60	233381.0
61	233381.0
62	204432.5
63	175484.0
64	175484.0
65	175484.0
66	143303.0
67	111122.0
68	111122.0
69	111122.0
70	84045.5
71	56969.0
72	56969.0
73	56969.0
74	43078.0
75	29187.0
76	29187.0
77	29187.0
78	22921.0
79	16655.0
80	16655.0
81	16655.0
82	11531.5
83	6408.0
84	6408.0
85	6408.0
86	4985.0
87	3562.0
88	3562.0
89	3562.0
90	2536.5
91	1511.0
92	1511.0
93	1511.0
94	1052.0
95	593.0
96	593.0
97	593.0
98	1054.5
99	1516.0
100	1516.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03763167576098831
2	0.005135239336254268
3	0.0014442860633215129
4	0.0035304770436748096
5	0.009107026010388429
6	0.013239288913780536
7	0.024272029675264314
8	0.03719036613052896
9	0.05139251241985717
10	0.05985763351321381
11	0.05761096630360257
12	0.06515334907872602
13	0.0658353730530723
14	0.06860358800777186
15	0.062184538837454034
16	0.06864370706508635
17	0.06398989641660592
18	0.07795132836204721
19	0.0762262088975243
20	0.08051894803017434
21	0.07518311340734765
22	0.07923513819611078
23	0.08324704392755942
24	0.0782722808205631
25	0.07935549536805424
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2492581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.646576604590486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.37684515963917	31.86731785213824
2	15.363166964501993	13.71825621539504
3	5.5880426646471895	7.484609246906721
4	2.537838733122054	4.532232456337226
5	1.389896228493757	3.102705421893896
6	0.8242460415576759	2.207985842126115
7	0.5353420590410702	1.6730833174045439
8	0.39098496005545186	1.3964911976286785
9	0.26628390806118596	1.0699798409841121
>10	1.4951292836614043	12.098097022330276
>50	0.12107430400340588	3.737070208561208
>100	0.09355155505617405	8.619193800251443
>500	0.011641845244034709	3.523265120178418
>1k	0.0056855523284817825	4.05729707131037
>5k	1.804937247137074E-4	0.5015564906965598
>10k+	9.02468623568537E-5	0.4108588958573924
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10197	0.4090940274358185	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7197	0.28873685549235917	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5251	0.21066516995836845	No Hit
GTACATGGGGTGGTATCAACGCAAA	3783	0.15177039382070232	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3590	0.14402741575900643	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3558	0.14274360592494284	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3445	0.1382101524484059	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2035717194345942E-4
2	0.0	0.0	0.0	0.0	1.2035717194345942E-4
3	0.0	0.0	0.0	0.0	1.2035717194345942E-4
4	0.0	0.0	0.0	0.0	1.2035717194345942E-4
5	0.0	0.0	0.0	0.0	1.6047622925794588E-4
6	0.0	0.0	0.0	0.0	1.6047622925794588E-4
7	0.0	0.0	0.0	0.0	1.6047622925794588E-4
8	0.0	0.0	0.0	0.0	1.6047622925794588E-4
9	0.0	0.0	0.0	4.011905731448647E-5	1.6047622925794588E-4
10	0.0	0.0	0.0	4.011905731448647E-5	1.6047622925794588E-4
11	0.0	0.0	0.0	8.023811462897294E-5	1.6047622925794588E-4
12	0.0	0.0	0.0	1.2035717194345942E-4	4.4130963045935115E-4
13	0.0	0.0	0.0	1.2035717194345942E-4	4.8142868777383766E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	170	0.0	16.203074	1
CGTTATT	180	0.0	13.716541	2
CGAACGA	150	1.4551915E-11	12.034631	16
CGCGCTA	80	2.8648241E-5	11.87628	16
GCACCGT	115	7.0851456E-8	11.564607	6
GATATAC	430	0.0	11.486382	1
ACCGTCT	75	2.07347E-4	11.400771	8
ATACCGT	450	0.0	11.399398	6
ACCGTCG	410	0.0	11.354425	8
ACTCTAA	410	0.0	11.122927	10
CGTCGTA	420	0.0	11.084306	10
ACGGTAT	335	0.0	11.060672	9
GTAGGAC	1320	0.0	11.007909	3
AAGACGG	450	0.0	10.976537	5
TATACTG	260	0.0	10.960299	5
TGTAGGA	1410	0.0	10.910375	2
TACGACA	70	0.001494357	10.855044	4
GTTATTC	245	0.0	10.853737	3
TTTTCGG	310	0.0	10.726963	16
CGCATCG	355	0.0	10.705164	13
>>END_MODULE