FastQCFastQC Report
Thu 2 Feb 2017
SRR4064079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492581
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25740.10326645352748817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG1502.1827873E-1120.5339826
GTATCAA70050.018.4987961
TAACCGT600.0074129118.3339125
TAGGACG13400.018.2226284
GTCCTAC15300.017.5430341
GTAGGAC18400.017.4553183
GATATAC6950.017.410621
TGTAGGA18550.017.0766492
GTCCTAA8950.016.4697911
AACGCAG78800.016.2476126
ACCGTCG5700.016.209418
TCCTACA16900.016.1405772
TACCGTC6550.016.1210277
ATTTCGT7650.016.10411342
AGGACGT14650.016.0671545
TACGCTG1105.6283992E-515.9988995
TAGGACC16450.015.9137984
GATTTCG7900.015.87264341
CTGTAGG19450.015.722861
ATACCGT6200.015.6118296
Produced by FastQC (version 0.11.5)