##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2492581
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265115556926737	32.0	32.0	32.0	32.0	32.0
2	31.419162707250035	32.0	32.0	32.0	32.0	32.0
3	31.497543309525348	32.0	32.0	32.0	32.0	32.0
4	31.585395620042036	32.0	32.0	32.0	32.0	32.0
5	31.52543809007611	32.0	32.0	32.0	32.0	32.0
6	35.12981684446764	36.0	36.0	36.0	36.0	36.0
7	35.14900418481887	36.0	36.0	36.0	36.0	36.0
8	35.109587612197956	36.0	36.0	36.0	36.0	36.0
9	35.205255917460654	36.0	36.0	36.0	36.0	36.0
10	35.06227360314469	36.0	36.0	36.0	36.0	36.0
11	35.209378551790294	36.0	36.0	36.0	36.0	36.0
12	35.11480148488655	36.0	36.0	36.0	36.0	36.0
13	35.15317897392301	36.0	36.0	36.0	36.0	36.0
14	35.099043120363994	36.0	36.0	36.0	36.0	36.0
15	35.07868309996746	36.0	36.0	36.0	36.0	36.0
16	35.08934353587707	36.0	36.0	36.0	36.0	36.0
17	35.04749935909806	36.0	36.0	36.0	36.0	36.0
18	35.04396446895808	36.0	36.0	36.0	36.0	36.0
19	35.04677079701723	36.0	36.0	36.0	36.0	36.0
20	35.03364023074877	36.0	36.0	36.0	36.0	36.0
21	35.03199575058945	36.0	36.0	36.0	36.0	36.0
22	35.01481757262853	36.0	36.0	36.0	36.0	36.0
23	34.96954562359257	36.0	36.0	36.0	36.0	36.0
24	34.94219686341186	36.0	36.0	36.0	36.0	36.0
25	34.91819884689806	36.0	36.0	36.0	36.0	36.0
26	34.86552051869127	36.0	36.0	36.0	32.0	36.0
27	34.85616234738209	36.0	36.0	36.0	32.0	36.0
28	34.83330491566774	36.0	36.0	36.0	32.0	36.0
29	34.79654141630703	36.0	36.0	36.0	32.0	36.0
30	34.773754995324126	36.0	36.0	36.0	32.0	36.0
31	34.763167174908254	36.0	36.0	36.0	32.0	36.0
32	34.71594463730567	36.0	36.0	36.0	32.0	36.0
33	34.692197364900075	36.0	36.0	36.0	32.0	36.0
34	34.6697182558962	36.0	36.0	36.0	32.0	36.0
35	34.62666890263546	36.0	36.0	36.0	32.0	36.0
36	34.59796532188924	36.0	36.0	36.0	32.0	36.0
37	34.57158423337095	36.0	36.0	36.0	32.0	36.0
38	34.53224870124582	36.0	36.0	36.0	32.0	36.0
39	34.50840113119694	36.0	36.0	36.0	32.0	36.0
40	34.49114151154968	36.0	36.0	36.0	32.0	36.0
41	34.468083083358174	36.0	36.0	36.0	32.0	36.0
42	34.40266855921633	36.0	36.0	36.0	32.0	36.0
43	34.38240963884424	36.0	36.0	36.0	32.0	36.0
44	34.301755890781486	36.0	36.0	36.0	32.0	36.0
45	34.259018663786655	36.0	36.0	36.0	32.0	36.0
46	34.20174188923048	36.0	36.0	36.0	32.0	36.0
47	34.153669228803395	36.0	36.0	36.0	32.0	36.0
48	34.093414015432195	36.0	36.0	36.0	32.0	36.0
49	34.055319365749796	36.0	36.0	36.0	32.0	36.0
50	33.47201114025984	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	14.0
20	19.0
21	72.0
22	251.0
23	792.0
24	1961.0
25	4512.0
26	9417.0
27	17276.0
28	28932.0
29	44065.0
30	62726.0
31	88431.0
32	127654.0
33	205645.0
34	425755.0
35	1475055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.91825038457804	18.008082357878656	12.00597670785149	26.067690549691815
2	15.555532375581377	20.524373809713897	38.096777907808516	25.823315906896205
3	18.36138064506811	24.993560873217806	28.67702222682255	27.968036254891533
4	12.08426125369647	16.203084272888223	36.140570757780786	35.57208371563452
5	13.829119294418115	37.436416309038705	33.42828176897762	15.306182627565565
6	33.66626881839062	35.93384351524026	17.035756195901826	13.364131470467289
7	29.699106949425897	30.67817281692062	20.963620024231922	18.659100209421563
8	27.778635879837005	33.60179669186277	19.36394444152467	19.255622986775556
9	27.758480271208203	13.898457403061121	18.57615694770416	39.76690537802652
10	15.858660748243972	27.16789512452278	31.69128396714056	25.282160160092694
11	36.86322479648589	21.091528091988256	23.12500426265326	18.920242848872594
12	25.25038313715107	23.624758282582707	29.282871562798384	21.841987017467844
13	29.91851339808879	19.864460471716395	25.42224711410812	24.794779016086693
14	23.498078692949537	19.947853102856726	25.737410403863635	30.8166578003301
15	24.641446163462916	28.03085165839299	22.723929895287476	24.60377228285662
16	24.839331787131215	25.88091194631702	24.841417996524857	24.438338270026907
17	23.363542995610974	26.25331985332467	25.820595527525697	24.562541623538664
18	24.11648667222718	24.989077569118226	27.532389169923338	23.36204658873126
19	25.526583320562086	24.560114066641045	26.075011494123757	23.838291118673116
20	25.853569882542494	24.353185338292622	25.84045084553598	23.952793933628907
21	26.551674749988063	23.996893180201567	25.081391537526766	24.370040532283603
22	25.768672341917622	24.625839041281374	25.51925176233272	24.086236854468286
23	24.149730277521684	24.597863563674643	26.403836694934768	24.848569463868902
24	24.821730182101202	24.83051629442704	26.19645586683624	24.15129765663552
25	24.609412925822358	24.72812764540447	26.40044452825041	24.262014900522765
26	23.894985508269755	25.64875769120899	26.756580657792956	23.699676142728297
27	24.576886272952137	25.226858288045932	25.878956754794046	24.317298684207884
28	23.83719740612905	25.223320766253522	26.412893767720725	24.526588059896707
29	24.080577248281354	25.015857477286996	26.056693741060737	24.846871533370912
30	23.769527261435112	25.291467082745395	26.47703469279757	24.461970963021926
31	24.756479112050503	24.91845189805464	25.613927813981256	24.7111411759136
32	24.334859790361577	25.38353336152478	25.466783711978664	24.814823136134986
33	23.864976302728085	25.09323039975029	26.19482355131293	24.846969746208696
34	24.644301624358924	25.144684574588233	26.34352541293028	23.867488388122567
35	24.86846571282075	25.134864269133157	26.191551795662676	23.805118222383417
36	23.87205623619523	25.571820915643702	26.104491954364264	24.451630893796803
37	24.72696883145749	25.41535923443348	25.474777942138775	24.38289399197025
38	24.27800908288828	25.632337617994693	25.77914514606326	24.31050815305377
39	24.959247334476775	25.03575763046771	25.44900149449844	24.55599354055708
40	25.29448155999801	25.287179701153374	25.777407246332622	23.640931492515996
41	24.288516402989284	25.409758784643387	26.299587202101666	24.002137610265663
42	25.031734747261424	26.04985444543228	25.58683204575581	23.331578761550485
43	24.247925508888727	25.268531405125454	25.966534525638313	24.5170085603475
44	23.821569219017295	25.79978271265755	25.549436882359927	24.829211185965228
45	24.014812169890615	25.961497184604493	25.512117133291472	24.51157351221342
46	24.09623859854647	25.356471572342436	25.496689140259694	25.0506006888514
47	24.67533874338471	25.341571846335498	25.64415706026148	24.338932350018315
48	24.822469681432374	25.83481375565088	24.89777463238185	24.444941930534895
49	24.452502179480028	25.88668629569436	25.006910546436668	24.653900978388947
50	24.219033344138857	26.39218760531375	24.871737881496557	24.517041169050835
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	60.5
2	60.0
3	198.5
4	337.0
5	396.0
6	455.0
7	510.5
8	566.0
9	765.0
10	964.0
11	1456.5
12	1949.0
13	3692.0
14	5435.0
15	7589.5
16	9744.0
17	10816.0
18	11888.0
19	12129.5
20	12371.0
21	13092.5
22	13814.0
23	14942.5
24	16071.0
25	16722.0
26	17373.0
27	21345.5
28	25318.0
29	30464.5
30	35611.0
31	41636.5
32	47662.0
33	56865.0
34	66068.0
35	76363.0
36	86658.0
37	96439.5
38	106221.0
39	110723.0
40	115225.0
41	121001.5
42	126778.0
43	128402.0
44	130026.0
45	141568.5
46	153111.0
47	162857.5
48	172604.0
49	178663.0
50	184722.0
51	180190.0
52	175658.0
53	173318.5
54	170979.0
55	172132.0
56	173285.0
57	168273.5
58	163262.0
59	148817.5
60	134373.0
61	117633.5
62	100894.0
63	87524.0
64	74154.0
65	62686.5
66	51219.0
67	43621.0
68	36023.0
69	32008.5
70	27994.0
71	21539.5
72	15085.0
73	12959.0
74	10833.0
75	8401.0
76	5969.0
77	5208.0
78	4447.0
79	3776.0
80	3105.0
81	2476.5
82	1848.0
83	1531.0
84	1214.0
85	874.5
86	535.0
87	393.5
88	252.0
89	190.0
90	128.0
91	106.5
92	85.0
93	67.0
94	49.0
95	46.0
96	43.0
97	36.0
98	29.0
99	27.5
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009588454698162267
2	3.2095245851589176E-4
3	2.808334012014053E-4
4	0.0
5	0.0
6	2.4071434388691883E-4
7	4.011905731448647E-5
8	0.0
9	0.0012436907767490806
10	0.0010029764328621617
11	8.023811462897294E-5
12	4.011905731448647E-5
13	9.628573755476753E-4
14	2.808334012014053E-4
15	0.005536429909399133
16	8.826192609187024E-4
17	4.011905731448647E-5
18	1.6047622925794588E-4
19	1.2035717194345942E-4
20	8.023811462897295E-4
21	0.0
22	1.6047622925794588E-4
23	7.622620889752429E-4
24	4.413096304593512E-4
25	0.00204607192303881
26	0.005014882164310809
27	0.006659763514204754
28	0.003610715158303782
29	0.002647857782756107
30	0.0041723819607065935
31	0.00750226371780897
32	0.0039717866741341605
33	0.004011905731448647
34	0.003610715158303782
35	0.0030089292985864854
36	0.009468097526218808
37	0.004011905731448647
38	0.008104049577526267
39	0.004252620075335565
40	0.0026077387254416205
41	0.0031292864705299447
42	0.001805357579151891
43	0.0016850004072084318
44	0.0011634526621201076
45	0.0014442860633215129
46	0.0010430954901766484
47	0.002407143438869188
48	0.001965833808409837
49	5.616668024028106E-4
50	0.0011634526621201076
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2492581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.07896033754668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.99074043442764	35.82309654789439
2	14.85132281567432	14.577749668611641
3	5.1830150847859455	7.631309753253468
4	2.3986869658722094	4.709002498409295
5	1.2367574777319112	3.0349385598384377
6	0.7722623986270665	2.274110137943788
7	0.49287075171800027	1.693270885257319
8	0.3400656847394996	1.3352056202792446
9	0.2508917527956084	1.108215574602591
>10	1.3019208812159095	11.43804620046452
>50	0.09246346933433403	3.1623189702440357
>100	0.07665070817517625	7.666185189781041
>500	0.008563758599035604	2.866812235477361
>1k	0.0037878163034184885	2.679738157942835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2574	0.10326645352748817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.011905731448647E-5
2	0.0	0.0	0.0	0.0	4.011905731448647E-5
3	0.0	0.0	0.0	0.0	4.011905731448647E-5
4	0.0	0.0	0.0	4.011905731448647E-5	4.011905731448647E-5
5	0.0	0.0	0.0	4.011905731448647E-5	4.011905731448647E-5
6	0.0	0.0	0.0	4.011905731448647E-5	4.011905731448647E-5
7	0.0	0.0	0.0	4.011905731448647E-5	4.011905731448647E-5
8	0.0	0.0	0.0	4.011905731448647E-5	4.011905731448647E-5
9	0.0	0.0	0.0	8.023811462897294E-5	4.011905731448647E-5
10	0.0	0.0	0.0	8.023811462897294E-5	8.023811462897294E-5
11	0.0	0.0	0.0	8.023811462897294E-5	8.023811462897294E-5
12	0.0	0.0	0.0	1.2035717194345942E-4	3.2095245851589176E-4
13	0.0	0.0	0.0	1.2035717194345942E-4	4.4130963045935115E-4
14	0.0	0.0	0.0	1.2035717194345942E-4	4.4130963045935115E-4
15	0.0	0.0	0.0	1.6047622925794588E-4	7.221430316607564E-4
16	0.0	0.0	0.0	1.6047622925794588E-4	7.62262088975243E-4
17	0.0	0.0	0.0	2.808334012014053E-4	7.62262088975243E-4
18	0.0	0.0	0.0	2.808334012014053E-4	9.628573755476753E-4
19	0.0	0.0	0.0	2.808334012014053E-4	9.628573755476753E-4
20	0.0	0.0	0.0	2.808334012014053E-4	0.0010832145474911348
21	0.0	0.0	0.0	4.011905731448647E-4	0.0011634526621201076
22	0.0	0.0	0.0	6.01785859717297E-4	0.0012436907767490806
23	0.0	0.0	0.0	0.0013239288913780534	0.0014041670060070265
24	0.0	0.0	0.0	0.00204607192303881	0.0014844051206359993
25	0.0	0.0	0.0	0.002407143438869188	0.0014844051206359993
26	0.0	0.0	0.0	0.003169405527844431	0.0017251194645229182
27	0.0	0.0	0.0	0.0045735725338514575	0.0017652385218374046
28	0.0	0.0	0.0	0.008104049577526267	0.0017652385218374046
29	0.0	0.0	0.0	0.015124884607561399	0.0018053575791518912
30	0.0	0.0	0.0	0.02764203048968118	0.0018053575791518912
31	0.0	0.0	0.0	0.05981751445589933	0.0018053575791518912
32	0.0	0.0	0.0	0.0946007371475591	0.0018053575791518912
33	0.0	0.0	0.0	0.12412836333102113	0.0018454766364663776
34	0.0	0.0	0.0	0.15698587127158556	0.001965833808409837
35	0.0	0.0	0.0	0.19694445235681407	0.001965833808409837
36	0.0	0.0	0.0	0.2521482752215475	0.001965833808409837
37	0.0	0.0	0.0	0.3300996035835947	0.001965833808409837
38	0.0	0.0	0.0	0.4361743911230969	0.0020861909803532963
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATCG	150	2.1827873E-11	20.53398	26
GTATCAA	7005	0.0	18.498796	1
TAACCGT	60	0.00741291	18.33391	25
TAGGACG	1340	0.0	18.222628	4
GTCCTAC	1530	0.0	17.543034	1
GTAGGAC	1840	0.0	17.455318	3
GATATAC	695	0.0	17.41062	1
TGTAGGA	1855	0.0	17.076649	2
GTCCTAA	895	0.0	16.469791	1
AACGCAG	7880	0.0	16.247612	6
ACCGTCG	570	0.0	16.20941	8
TCCTACA	1690	0.0	16.140577	2
TACCGTC	655	0.0	16.121027	7
ATTTCGT	765	0.0	16.104113	42
AGGACGT	1465	0.0	16.067154	5
TACGCTG	110	5.6283992E-5	15.998899	5
TAGGACC	1645	0.0	15.913798	4
GATTTCG	790	0.0	15.872643	41
CTGTAGG	1945	0.0	15.72286	1
ATACCGT	620	0.0	15.611829	6
>>END_MODULE