FastQCFastQC Report
Thu 2 Feb 2017
SRR4064078_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064078_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956845
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27250.2847901175216467No Hit
TATCAACGCAGAGTACTTTTTTTTT19810.20703457717812182No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17570.18362430696716814No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11340.11851449294295313No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10080.10534621594929167No Hit
GGTATCAACGCAGAGTACTTTTTTT10030.10482366527494004No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9800.10241993217292246No Hit
GATTAAGAGGGACGGCCGGGGGCAT9730.10168836122883015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTC1100.017.2666913
CGTTATT1000.017.0958122
GCGTTAT1150.016.5202831
CGGTCCA1900.014.500998510
GAGCGAC551.9568988E-413.81985511
GGTTCTA2150.013.25672613
GTCTTAA655.4527125E-513.1526871
TTTTCGG1102.733941E-912.95679216
CCGGTCC2000.012.824549
CGCCGGT2000.012.824547
ACGCCCC1051.9863364E-812.668200517
AGCGTAT1051.990702E-812.6662148
CCAGTAC604.1015437E-412.662243
CGACCAT1155.305992E-912.39215710
GTCTTAG1001.4418038E-712.3489111
GCCGGTC2100.012.2138498
GCTCGTA701.0904182E-412.2138489
ACGAACG550.003062979612.09300615
ATAACGA550.0030641812.09237312
CCGTCGT1103.808418E-812.0904769
Produced by FastQC (version 0.11.5)