FastQCFastQC Report
Thu 2 Feb 2017
SRR4064078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956845
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10340.10806347945592024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGGT300.00574552229.33218611
GTATTAA1306.91216E-1122.0002881
TATACCG500.00258063521.9991385
TACACCG500.00258063521.9991385
ATAATAC956.865812E-720.8412883
ACTGCGT550.004483957319.9992168
TACGCTG550.004483957319.9992165
TAGTACA708.1237743E-418.8564034
GTTATAT951.5963633E-518.5265581
TACACTA600.007412675318.3326155
GTCTTAC851.4302945E-418.1178841
TATACTG851.4308671E-418.1169385
CGATTTG2450.017.96035628
GTCCTAA2150.017.3955761
AGCGATT2550.017.25783326
GCGATTT2550.017.25783327
ATACCGT2950.017.151876
TAATACC902.215427E-417.1104414
TAGCCTA902.215427E-417.1104415
TTAGTTG2500.016.72021915
Produced by FastQC (version 0.11.5)