##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064078_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	956845
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300893039102466	32.0	32.0	32.0	32.0	32.0
2	31.416176078675228	32.0	32.0	32.0	32.0	32.0
3	31.4886967063631	32.0	32.0	32.0	32.0	32.0
4	31.57317851898688	32.0	32.0	32.0	32.0	32.0
5	31.528038501533686	32.0	32.0	32.0	32.0	32.0
6	35.1080373519222	36.0	36.0	36.0	36.0	36.0
7	35.12717629292101	36.0	36.0	36.0	36.0	36.0
8	35.074248180217275	36.0	36.0	36.0	36.0	36.0
9	35.17519451948853	36.0	36.0	36.0	36.0	36.0
10	35.03691402473755	36.0	36.0	36.0	36.0	36.0
11	35.177621244820216	36.0	36.0	36.0	36.0	36.0
12	35.08843334082323	36.0	36.0	36.0	36.0	36.0
13	35.12309830745836	36.0	36.0	36.0	36.0	36.0
14	35.06298094257691	36.0	36.0	36.0	36.0	36.0
15	35.05184747790917	36.0	36.0	36.0	36.0	36.0
16	35.056886956612615	36.0	36.0	36.0	36.0	36.0
17	35.0112055766608	36.0	36.0	36.0	36.0	36.0
18	35.01321008104761	36.0	36.0	36.0	36.0	36.0
19	35.01407437986299	36.0	36.0	36.0	36.0	36.0
20	35.00269009087156	36.0	36.0	36.0	36.0	36.0
21	34.989859381613535	36.0	36.0	36.0	36.0	36.0
22	34.98869722891377	36.0	36.0	36.0	36.0	36.0
23	34.93580360455455	36.0	36.0	36.0	36.0	36.0
24	34.90891837235916	36.0	36.0	36.0	32.0	36.0
25	34.876141903861125	36.0	36.0	36.0	32.0	36.0
26	34.8144694281728	36.0	36.0	36.0	32.0	36.0
27	34.81435446702444	36.0	36.0	36.0	32.0	36.0
28	34.77937283468064	36.0	36.0	36.0	32.0	36.0
29	34.75040889590268	36.0	36.0	36.0	32.0	36.0
30	34.730431783622215	36.0	36.0	36.0	32.0	36.0
31	34.73561444121044	36.0	36.0	36.0	32.0	36.0
32	34.68219931127821	36.0	36.0	36.0	32.0	36.0
33	34.65044599700056	36.0	36.0	36.0	32.0	36.0
34	34.638802522874656	36.0	36.0	36.0	32.0	36.0
35	34.60822912801969	36.0	36.0	36.0	32.0	36.0
36	34.575567620670014	36.0	36.0	36.0	32.0	36.0
37	34.55771519943146	36.0	36.0	36.0	32.0	36.0
38	34.51607836169912	36.0	36.0	36.0	32.0	36.0
39	34.507593183849	36.0	36.0	36.0	32.0	36.0
40	34.49658931174851	36.0	36.0	36.0	32.0	36.0
41	34.48240833154795	36.0	36.0	36.0	32.0	36.0
42	34.40587242447836	36.0	36.0	36.0	32.0	36.0
43	34.38700207452618	36.0	36.0	36.0	32.0	36.0
44	34.323881088368545	36.0	36.0	36.0	32.0	36.0
45	34.28803202190532	36.0	36.0	36.0	32.0	36.0
46	34.25405055155224	36.0	36.0	36.0	32.0	36.0
47	34.21422382935585	36.0	36.0	36.0	32.0	36.0
48	34.170104875920345	36.0	36.0	36.0	32.0	36.0
49	34.15487670416839	36.0	36.0	36.0	32.0	36.0
50	33.54975570755974	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	13.0
21	37.0
22	110.0
23	363.0
24	855.0
25	1850.0
26	3884.0
27	6845.0
28	11403.0
29	17226.0
30	24329.0
31	34114.0
32	49417.0
33	78401.0
34	161991.0
35	566002.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.197281051708515	17.726781964678967	11.579621788794974	26.496315194817548
2	15.973913997711204	20.65277712458261	37.10775630072061	26.265552576985584
3	18.096072187391243	24.641764636413708	28.817266782533814	28.44489639366124
4	12.12641545913915	15.941975973119993	36.253520685168446	35.67808788257241
5	14.098103663602778	37.279183148785854	33.31051528721998	15.312197900391391
6	33.80826321559545	36.1363358461106	16.794500247166987	13.260900691126967
7	29.99325909630086	30.643834685868658	20.942890436800106	18.420015781030365
8	27.9713307498401	32.97434064487001	19.57560480078257	19.478723804507318
9	27.840385939866202	13.562554803189272	18.301417279713387	40.29564197723114
10	15.521331719685048	26.66595599255046	31.96215033265819	25.8505619551063
11	37.369584415448685	20.817791805360326	22.78843490847525	19.02418887071574
12	24.959345515047385	23.58284109008365	29.06450790306466	22.39330549180431
13	29.814086532482342	19.363260221373828	25.33278709255332	25.48986615359051
14	23.537170113414515	19.537876602664596	25.472281793061356	31.452671490859537
15	25.08711420478893	27.369433208959126	22.441288056940394	25.102164529311555
16	25.472781675457785	25.561720543833484	24.175172991847095	24.790324788861636
17	23.90042274349555	25.855389326379925	25.426375222737224	24.817812707387297
18	24.744811844785403	24.710845979957003	26.632477846417856	23.911864328839734
19	25.642397671514196	24.56709289383338	25.662150087004687	24.128359347647738
20	26.062246561598595	23.933991053885705	25.281969817315332	24.721792567200367
21	26.962256164791583	23.87492227058719	24.567510934372862	24.595310630248367
22	25.879844697939582	24.22764397577455	25.222789480009823	24.669721846276044
23	24.36347636695031	24.15623125335741	25.628685315591564	25.851607064100712
24	24.806734633091043	24.61098715047892	25.649086320145898	24.933191896284143
25	24.872234357200337	24.337134078153905	25.645412455713135	25.145219108932622
26	24.55163043478261	25.24602842809365	25.85211120401338	24.350229933110366
27	25.146374245657892	24.765937077364615	25.200513381145782	24.887175295831703
28	24.388049125381343	24.682880843463277	25.881333630150806	25.047736401004585
29	24.56232944931529	24.696000426409373	25.67768259047874	25.0639875337966
30	24.42342322569452	24.920595603520447	25.923301467060895	24.73267970372414
31	25.10041566808428	24.71537135767836	24.908207066880024	25.276005907357334
32	24.757658513541337	24.923417477432846	24.897602556826794	25.421321452199024
33	24.417256908626676	24.522606868023047	25.5838404867837	25.476295736566573
34	25.12831500013064	24.690303869568623	25.584615786585847	24.596765343714893
35	25.63630111860237	24.450183368014223	25.659921050898866	24.253594462484546
36	24.657495589354248	25.148728647039643	25.282824820020565	24.91095094358554
37	25.473994450337837	24.984558143423754	24.914011590537356	24.62743581570105
38	24.642882899455348	24.95121550986194	25.37096593813071	25.034935652551997
39	25.335464026780684	24.65026980306414	24.996420393202822	25.017845776952345
40	25.73624242018858	24.826456024029646	25.33533022999149	24.101971325790274
41	24.556707754243753	25.07122566674122	25.845667619129237	24.526398959885785
42	25.558592219057825	25.341206594727357	25.157264912601573	23.942936273613252
43	24.86870694763641	24.478146042814338	25.552423682810616	25.100723326738635
44	24.491107643653596	25.239200571050542	24.92545721144651	25.344234573849356
45	24.75181196340017	25.49467776575899	24.925718645325475	24.82779162551537
46	24.553163649437	24.914562339706407	25.073732439556125	25.45854157130047
47	24.97199078622281	24.796933200115383	25.45420911595396	24.776866897707844
48	25.35233936617061	25.331750310142255	24.51184602312454	24.804064300562594
49	24.616262506022434	25.495929827275017	24.78118053298413	25.106627133718423
50	24.907062808506016	25.83606893185721	24.462573772325044	24.794294487311735
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	119.0
1	76.0
2	33.0
3	125.5
4	218.0
5	224.5
6	231.0
7	223.0
8	215.0
9	329.5
10	444.0
11	583.5
12	723.0
13	1356.5
14	1990.0
15	2519.0
16	3048.0
17	3158.0
18	3268.0
19	3211.5
20	3155.0
21	3379.0
22	3603.0
23	4048.5
24	4494.0
25	4895.0
26	5296.0
27	6490.5
28	7685.0
29	9323.5
30	10962.0
31	13107.5
32	15253.0
33	18199.0
34	21145.0
35	24526.0
36	27907.0
37	31731.5
38	35556.0
39	38730.0
40	41904.0
41	45725.0
42	49546.0
43	51142.5
44	52739.0
45	57919.5
46	63100.0
47	66312.5
48	69525.0
49	72059.0
50	74593.0
51	72219.0
52	69845.0
53	68941.5
54	68038.0
55	68150.5
56	68263.0
57	66225.5
58	64188.0
59	58280.5
60	52373.0
61	46126.0
62	39879.0
63	34764.5
64	29650.0
65	25243.5
66	20837.0
67	18054.5
68	15272.0
69	13780.5
70	12289.0
71	9660.0
72	7031.0
73	5867.0
74	4703.0
75	3692.0
76	2681.0
77	2362.5
78	2044.0
79	1675.0
80	1306.0
81	1030.0
82	754.0
83	639.5
84	525.0
85	365.0
86	205.0
87	142.0
88	79.0
89	60.5
90	42.0
91	35.5
92	29.0
93	22.0
94	15.0
95	17.0
96	19.0
97	15.5
98	12.0
99	13.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008151790519885665
2	0.0010451013487032905
3	2.0902026974065811E-4
4	0.0
5	0.0
6	2.0902026974065811E-4
7	0.0
8	1.0451013487032906E-4
9	0.0012541216184439486
10	7.315709440923033E-4
11	0.0
12	1.0451013487032906E-4
13	2.0902026974065811E-4
14	1.0451013487032906E-4
15	0.005748057417868098
16	8.360810789626325E-4
17	0.0
18	2.0902026974065811E-4
19	0.0
20	5.225506743516453E-4
21	0.0
22	0.0
23	7.315709440923033E-4
24	0.0
25	0.0015676520230549357
26	0.004702956069164807
27	0.005330016878386782
28	0.0029262837763692133
29	0.001881182427665923
30	0.0035533445855911875
31	0.007524729710663692
32	0.0035533445855911875
33	0.0035533445855911875
34	0.002090202697406581
35	0.0027172635066285552
36	0.007211199306052704
37	0.0031353040461098713
38	0.008987871598848299
39	0.003762364855331846
40	0.0028217736414988845
41	0.0028217736414988845
42	0.002090202697406581
43	0.001672162157925265
44	0.0012541216184439486
45	0.0010451013487032905
46	7.315709440923033E-4
47	0.0017766722927955937
48	0.0027172635066285552
49	6.270608092219743E-4
50	0.001672162157925265
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	956845.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.9636780499343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.74545698676648	47.020854517125834
2	12.386828576246115	14.60745944459004
3	3.503259179875419	6.196951392229531
4	1.4660745368767456	3.4578058795842788
5	0.7419538792525535	2.18741648320737
6	0.4617003868600721	1.6334131779808443
7	0.31475473407169463	1.2991367763147261
8	0.2200233202348979	1.0378707374246492
9	0.1591465628822877	0.8445480026890353
>10	0.8671196360854646	9.13157014510333
>50	0.06559433690003787	2.7331280354521423
>100	0.06183307344482749	7.318997013256292
>500	0.006076082203432705	2.421892726773436
>1k	1.787083001009619E-4	0.108955668268523
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1034	0.10806347945592024	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.090202697406581E-4
2	0.0	0.0	0.0	0.0	2.090202697406581E-4
3	0.0	0.0	0.0	0.0	2.090202697406581E-4
4	0.0	0.0	0.0	0.0	2.090202697406581E-4
5	0.0	0.0	0.0	0.0	4.180405394813162E-4
6	0.0	0.0	0.0	0.0	5.225506743516453E-4
7	0.0	0.0	0.0	0.0	5.225506743516453E-4
8	0.0	0.0	0.0	0.0	7.315709440923033E-4
9	0.0	0.0	0.0	0.0	7.315709440923033E-4
10	0.0	0.0	0.0	0.0	7.315709440923033E-4
11	0.0	0.0	0.0	0.0	7.315709440923033E-4
12	0.0	0.0	0.0	0.0	0.0011496114835736196
13	0.0	0.0	0.0	0.0	0.0011496114835736196
14	0.0	0.0	0.0	0.0	0.0012541216184439486
15	0.0	0.0	0.0	0.0	0.0016721621579252647
16	0.0	0.0	0.0	0.0	0.001881182427665923
17	0.0	0.0	0.0	0.0	0.001881182427665923
18	0.0	0.0	0.0	0.0	0.001881182427665923
19	0.0	0.0	0.0	0.0	0.001881182427665923
20	0.0	0.0	0.0	2.090202697406581E-4	0.001881182427665923
21	0.0	0.0	0.0	3.1353040461098715E-4	0.001881182427665923
22	0.0	0.0	0.0	5.225506743516453E-4	0.001881182427665923
23	0.0	0.0	0.0	0.0015676520230549357	0.001881182427665923
24	0.0	0.0	0.0	0.0028217736414988845	0.001881182427665923
25	0.0	0.0	0.0	0.0038668749902021748	0.001881182427665923
26	0.0	0.0	0.0	0.00438942566455382	0.001881182427665923
27	0.0	0.0	0.0	0.006061587822479085	0.001985692562536252
28	0.0	0.0	0.0	0.008465320924496653	0.001985692562536252
29	0.0	0.0	0.0	0.014631418881846067	0.001985692562536252
30	0.0	0.0	0.0	0.026127533717582262	0.001985692562536252
31	0.0	0.0	0.0	0.049955844468017284	0.001985692562536252
32	0.0	0.0	0.0	0.07786005047839514	0.001985692562536252
33	0.0	0.0	0.0	0.09991168893603457	0.001985692562536252
34	0.0	0.0	0.0	0.1284429557556344	0.002090202697406581
35	0.0	0.0	0.0	0.15843736446341883	0.002090202697406581
36	0.0	0.0	0.0	0.20818418866169547	0.002090202697406581
37	0.0	0.0	0.0	0.2781014688899456	0.002090202697406581
38	0.0	0.0	0.0	0.3719515700035011	0.002299222967147239
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGGT	30	0.005745522	29.332186	11
GTATTAA	130	6.91216E-11	22.000288	1
TATACCG	50	0.002580635	21.999138	5
TACACCG	50	0.002580635	21.999138	5
ATAATAC	95	6.865812E-7	20.841288	3
ACTGCGT	55	0.0044839573	19.999216	8
TACGCTG	55	0.0044839573	19.999216	5
TAGTACA	70	8.1237743E-4	18.856403	4
GTTATAT	95	1.5963633E-5	18.526558	1
TACACTA	60	0.0074126753	18.332615	5
GTCTTAC	85	1.4302945E-4	18.117884	1
TATACTG	85	1.4308671E-4	18.116938	5
CGATTTG	245	0.0	17.960356	28
GTCCTAA	215	0.0	17.395576	1
AGCGATT	255	0.0	17.257833	26
GCGATTT	255	0.0	17.257833	27
ATACCGT	295	0.0	17.15187	6
TAATACC	90	2.215427E-4	17.110441	4
TAGCCTA	90	2.215427E-4	17.110441	5
TTAGTTG	250	0.0	16.720219	15
>>END_MODULE