FastQCFastQC Report
Thu 2 Feb 2017
SRR4064077_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064077_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583386
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32790.5620635394061564No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25690.44036024176102956No Hit
TATCAACGCAGAGTACTTTTTTTTT24730.4239045846146462No Hit
GTACATGGAAGCAGTGGTATCAACG18820.32259944530722373No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17060.2924307405388542No Hit
GTACATGGGGTGGTATCAACGCAAA16270.2788891060121429No Hit
TCCATGTACTCTGCGTTGATACCAC15650.26826149410510364No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13670.23432170124068796No Hit
GGTATCAACGCAGAGTACTTTTTTT11830.2027816917101199No Hit
GTCCTAAAGTGTGTATTTCTCATTT10170.17432711789449867No Hit
CCCATGTACTCTGCGTTGATACCAC10010.17158450837010145No Hit
GTACATGGGAAGCAGTGGTATCAAC9090.15581450360481738No Hit
CTGTAGGACGTGGAATATGGCAAGA8440.14467265241195365No Hit
GTACATGGGTGGTATCAACGCAAAA8310.14244428217338093No Hit
GTCCTACAGTGGACATTTCTAAATT8290.14210145598283125No Hit
GTATCAACGCAGAGTACATGGGGTG8170.14004449883953335No Hit
GTACATGGGAGTGGTATCAACGCAA7150.12256036312150104No Hit
CTTTAGGACGTGAAATATGGCGAGG7080.12136047145457725No Hit
GAGTACATGGAAGCAGTGGTATCAA7010.12016057978765345No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6770.11604666550105762No Hit
CATGTACTCTGCGTTGATACCACTG6610.1133040559766604No Hit
GTATCAACGCAGAGTACATGGGAAG6360.10901872859478974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC555.8926344E-717.27311711
GTATTAG555.903239E-717.2701531
AATACTG453.5424942E-516.8834745
GTTACGC350.002167683616.2860819
TCGCGTA651.9067738E-716.0772889
TCGGGGG651.9198524E-716.0676332
CGCGTAA704.4167246E-714.9301910
CGGCTCG400.00527307314.2503218
CTAGGAC602.5747546E-514.2429883
GTCGCGT759.640407E-713.9336478
TAGGACT551.9636039E-413.8125664
GATTAAG1257.2759576E-1213.6779611
TCGCCAG852.6800808E-713.41552217
CTTTCGT500.001494427213.30372516
GTAAGAC500.001503152513.2934553
CTTAATG500.001504124613.2923152
TATTAGC500.001504124613.2923152
ATACTGT655.4530894E-513.1507586
TCTAGGA655.4706805E-513.1462452
CGCATCG801.9889467E-613.06503813
Produced by FastQC (version 0.11.5)