##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064077_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	583386
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.171536512703426	32.0	32.0	32.0	32.0	32.0
2	30.776952138035536	32.0	32.0	32.0	32.0	32.0
3	30.803341184053096	32.0	32.0	32.0	32.0	32.0
4	30.813687678483884	32.0	32.0	32.0	32.0	32.0
5	30.72209137689283	32.0	32.0	32.0	32.0	32.0
6	34.36582125728077	36.0	36.0	36.0	32.0	36.0
7	34.26846547568848	36.0	36.0	36.0	32.0	36.0
8	34.20204118713853	36.0	36.0	36.0	32.0	36.0
9	34.35499652031417	36.0	36.0	36.0	32.0	36.0
10	34.03395007765013	36.0	36.0	36.0	32.0	36.0
11	34.404973722372496	36.0	36.0	36.0	32.0	36.0
12	34.18309489771781	36.0	36.0	36.0	32.0	36.0
13	34.255222099947545	36.0	36.0	36.0	32.0	36.0
14	34.163327539570716	36.0	36.0	36.0	32.0	36.0
15	34.10936498304724	36.0	36.0	36.0	32.0	36.0
16	34.08905938778099	36.0	36.0	36.0	32.0	36.0
17	34.01648136911067	36.0	36.0	36.0	32.0	36.0
18	34.02280651232631	36.0	36.0	36.0	32.0	36.0
19	34.01644708649162	36.0	36.0	36.0	32.0	36.0
20	33.97900360995979	36.0	36.0	36.0	32.0	36.0
21	33.971812830613004	36.0	36.0	36.0	32.0	36.0
22	33.92411885098374	36.0	36.0	36.0	32.0	36.0
23	33.86362888379221	36.0	36.0	36.0	32.0	36.0
24	33.88801239659505	36.0	36.0	36.0	32.0	36.0
25	33.4575238349909	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	7.0
5	15.0
6	19.0
7	9.0
8	17.0
9	30.0
10	50.0
11	21.0
12	46.0
13	26.0
14	64.0
15	136.0
16	188.0
17	246.0
18	321.0
19	463.0
20	688.0
21	1008.0
22	1538.0
23	2375.0
24	3452.0
25	5117.0
26	6998.0
27	9370.0
28	12280.0
29	16770.0
30	22099.0
31	30329.0
32	41972.0
33	58913.0
34	122632.0
35	246186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.57428302935104	17.036669936276898	11.384759958020949	25.00428707635112
2	15.406610473697475	20.265810915287126	39.665413553168186	24.662165057847215
3	19.028689852359623	25.863035395636096	28.396902860414446	26.71137189158983
4	11.813218366174972	15.810612748669337	36.6150971535343	35.7610717316214
5	13.051296952491647	38.49971456930823	34.05968158620607	14.389306891994055
6	32.36277856162	36.08979117414612	18.143385409791378	13.404044854442498
7	28.94209865790683	31.105255217155747	21.624536233772467	18.328109891164953
8	27.805203423223528	33.91947434211316	19.865121837882143	18.410200396781168
9	28.076466633739432	13.91792887718143	18.825458237295578	39.18014625178356
10	15.480739546530376	27.673995815181975	32.893184234898634	23.95208040338902
11	36.15034344369838	21.175683671631806	23.64710624029911	19.026866644370696
12	25.098882024225666	23.929621383902383	30.29717778867891	20.67431880319304
13	30.32356109308213	20.615013070056875	25.493643355539852	23.56778248132114
14	22.967910586007452	20.538048288495975	26.62917069756911	29.864870427927464
15	24.14015480901	28.871897516641198	24.003114703768688	22.98483297058011
16	23.559368841028423	26.19097035803297	26.891838810547675	23.35782199039093
17	22.410880143806164	26.702080764038854	27.210650987037894	23.67638810511709
18	23.089604394360205	25.170303759119893	29.52006161985422	22.22003022666568
19	24.47905855712901	25.590674775060684	27.03989295547531	22.890373712334995
20	25.01921361421807	24.690695121114388	27.58080010979208	22.709291154875455
21	25.41712270252281	24.7336693416958	25.771372499236605	24.077835456544786
22	24.714623426440262	26.060448024923062	26.058389402210274	23.166539146426402
23	22.7796697405104	26.078490242333263	27.121938666094792	24.01990135106155
24	23.789673043173945	26.1923999993138	27.21346460326528	22.804462354246972
25	23.787524510266437	25.81730909440248	27.03943429533091	23.35573210000017
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	142.0
1	142.0
2	343.0
3	544.0
4	544.0
5	544.0
6	1806.0
7	3068.0
8	3068.0
9	3068.0
10	2841.0
11	2614.0
12	2614.0
13	2614.0
14	2239.5
15	1865.0
16	1865.0
17	1865.0
18	3225.5
19	4586.0
20	4586.0
21	4586.0
22	7537.0
23	10488.0
24	10488.0
25	10488.0
26	16307.5
27	22127.0
28	22127.0
29	22127.0
30	27923.5
31	33720.0
32	33720.0
33	33720.0
34	37946.0
35	42172.0
36	42172.0
37	42172.0
38	46511.0
39	50850.0
40	50850.0
41	50850.0
42	57570.5
43	64291.0
44	64291.0
45	64291.0
46	74795.5
47	85300.0
48	85300.0
49	85300.0
50	82214.0
51	79128.0
52	79128.0
53	79128.0
54	68833.5
55	58539.0
56	58539.0
57	58539.0
58	52709.0
59	46879.0
60	46879.0
61	46879.0
62	40500.5
63	34122.0
64	34122.0
65	34122.0
66	27758.0
67	21394.0
68	21394.0
69	21394.0
70	15988.5
71	10583.0
72	10583.0
73	10583.0
74	7974.5
75	5366.0
76	5366.0
77	5366.0
78	4151.0
79	2936.0
80	2936.0
81	2936.0
82	2050.5
83	1165.0
84	1165.0
85	1165.0
86	925.0
87	685.0
88	685.0
89	685.0
90	487.5
91	290.0
92	290.0
93	290.0
94	206.5
95	123.0
96	123.0
97	123.0
98	266.0
99	409.0
100	409.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040796316675408735
2	0.006685110715718238
3	0.0015427178574734396
4	0.005313805953519625
5	0.009770546430665116
6	0.01319880833616165
7	0.02022674524242954
8	0.033082727388041536
9	0.04833849286750111
10	0.05588066905959347
11	0.05502360358321934
12	0.0630800190611362
13	0.06410849763278516
14	0.06685110715718237
15	0.059137517869815186
16	0.06770817263355651
17	0.0649655631091593
18	0.07833578454059577
19	0.0773073059689468
20	0.07987850239806919
21	0.07953567620751954
22	0.08090698096971816
23	0.0875920916854364
24	0.07970708930279438
25	0.08004991549334403
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	583386.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.06394847279955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7381299132605	47.096487965142146
2	13.125301514237735	15.504642646537912
3	3.608481104065507	6.393934259867878
4	1.3428532285870443	3.172568555991908
5	0.6077179704529669	1.794711144641418
6	0.3425969886666071	1.2141078531324463
7	0.20529338423889085	0.8487806507944741
8	0.13639521238838714	0.6444831837155398
9	0.11058944991483977	0.5878664614264808
>10	0.6225566443998243	7.07687950974626
>50	0.08864429712727108	3.5545802928721995
>100	0.06444205991005371	7.106748425698125
>500	0.00379070940647347	1.6166888200859244
>1k	0.0032075233439390898	3.387520230347294
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3279	0.5620635394061564	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2569	0.44036024176102956	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2473	0.4239045846146462	No Hit
GTACATGGAAGCAGTGGTATCAACG	1882	0.32259944530722373	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1706	0.2924307405388542	No Hit
GTACATGGGGTGGTATCAACGCAAA	1627	0.2788891060121429	No Hit
TCCATGTACTCTGCGTTGATACCAC	1565	0.26826149410510364	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1367	0.23432170124068796	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1183	0.2027816917101199	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1017	0.17432711789449867	No Hit
CCCATGTACTCTGCGTTGATACCAC	1001	0.17158450837010145	No Hit
GTACATGGGAAGCAGTGGTATCAAC	909	0.15581450360481738	No Hit
CTGTAGGACGTGGAATATGGCAAGA	844	0.14467265241195365	No Hit
GTACATGGGTGGTATCAACGCAAAA	831	0.14244428217338093	No Hit
GTCCTACAGTGGACATTTCTAAATT	829	0.14210145598283125	No Hit
GTATCAACGCAGAGTACATGGGGTG	817	0.14004449883953335	No Hit
GTACATGGGAGTGGTATCAACGCAA	715	0.12256036312150104	No Hit
CTTTAGGACGTGAAATATGGCGAGG	708	0.12136047145457725	No Hit
GAGTACATGGAAGCAGTGGTATCAA	701	0.12016057978765345	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	677	0.11604666550105762	No Hit
CATGTACTCTGCGTTGATACCACTG	661	0.1133040559766604	No Hit
GTATCAACGCAGAGTACATGGGAAG	636	0.10901872859478974	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.714130952748266E-4	0.0
13	0.0	0.0	0.0	5.142392858244799E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAC	55	5.8926344E-7	17.273117	11
GTATTAG	55	5.903239E-7	17.270153	1
AATACTG	45	3.5424942E-5	16.883474	5
GTTACGC	35	0.0021676836	16.286081	9
TCGCGTA	65	1.9067738E-7	16.077288	9
TCGGGGG	65	1.9198524E-7	16.067633	2
CGCGTAA	70	4.4167246E-7	14.93019	10
CGGCTCG	40	0.005273073	14.250321	8
CTAGGAC	60	2.5747546E-5	14.242988	3
GTCGCGT	75	9.640407E-7	13.933647	8
TAGGACT	55	1.9636039E-4	13.812566	4
GATTAAG	125	7.2759576E-12	13.677961	1
TCGCCAG	85	2.6800808E-7	13.415522	17
CTTTCGT	50	0.0014944272	13.303725	16
GTAAGAC	50	0.0015031525	13.293455	3
CTTAATG	50	0.0015041246	13.292315	2
TATTAGC	50	0.0015041246	13.292315	2
ATACTGT	65	5.4530894E-5	13.150758	6
TCTAGGA	65	5.4706805E-5	13.146245	2
CGCATCG	80	1.9889467E-6	13.065038	13
>>END_MODULE