FastQCFastQC Report
Thu 2 Feb 2017
SRR4064077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583386
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10820.1854689690873624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8960.15358613336624466No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA8410.14415841312612918No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC7900.13541634526711302No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA6300.10799025002314078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAT353.2153592E-431.42936727
CGTAATT353.2153592E-431.42936728
TCCGTAA450.001396443524.44715926
CGCGTAA551.5919658E-424.0006110
TCGCGTA551.5919658E-424.000619
GACGTGA3450.022.9531697
CGTCGTA703.211048E-522.00055710
CGGTTGG500.00257975721.9986740
TAATACC703.2153013E-521.9967864
TAGGACG6950.021.8385354
TTAGGAC4550.021.7550623
GTCCTAA4050.021.1839051
CGACCAT1158.745701E-921.04401210
AGGACGT7050.020.9047475
ATAGCGT956.863411E-720.839066
GCGTAAC755.4653494E-520.53385411
GATATAC1401.8371793E-1020.4273381
TTTAGGA5000.020.2370432
GTCCTAC6250.020.0627881
ACTATCC550.004484668419.997088
Produced by FastQC (version 0.11.5)