##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064077_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	583386
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.153174741937583	32.0	32.0	32.0	32.0	32.0
2	31.41514366131515	32.0	32.0	32.0	32.0	32.0
3	31.49526728443946	32.0	32.0	32.0	32.0	32.0
4	31.593497272817654	32.0	32.0	32.0	32.0	32.0
5	31.53722406776988	32.0	32.0	32.0	32.0	32.0
6	35.11976461553757	36.0	36.0	36.0	36.0	36.0
7	35.14864257969852	36.0	36.0	36.0	36.0	36.0
8	35.111691058750125	36.0	36.0	36.0	36.0	36.0
9	35.20136239128124	36.0	36.0	36.0	36.0	36.0
10	35.0760285642782	36.0	36.0	36.0	36.0	36.0
11	35.20638650910375	36.0	36.0	36.0	36.0	36.0
12	35.12945802607536	36.0	36.0	36.0	36.0	36.0
13	35.1564281624859	36.0	36.0	36.0	36.0	36.0
14	35.09507598742513	36.0	36.0	36.0	36.0	36.0
15	35.07588457727817	36.0	36.0	36.0	36.0	36.0
16	35.079940211112365	36.0	36.0	36.0	36.0	36.0
17	35.054471653416435	36.0	36.0	36.0	36.0	36.0
18	35.04068832642538	36.0	36.0	36.0	36.0	36.0
19	35.03591275759103	36.0	36.0	36.0	36.0	36.0
20	35.018764591539735	36.0	36.0	36.0	36.0	36.0
21	35.007986136108855	36.0	36.0	36.0	36.0	36.0
22	34.98599212185414	36.0	36.0	36.0	36.0	36.0
23	34.93879181193927	36.0	36.0	36.0	36.0	36.0
24	34.917572584875195	36.0	36.0	36.0	36.0	36.0
25	34.88004511592668	36.0	36.0	36.0	32.0	36.0
26	34.81168557353107	36.0	36.0	36.0	32.0	36.0
27	34.80336689601739	36.0	36.0	36.0	32.0	36.0
28	34.77271823458225	36.0	36.0	36.0	32.0	36.0
29	34.73833962419393	36.0	36.0	36.0	32.0	36.0
30	34.70512833698443	36.0	36.0	36.0	32.0	36.0
31	34.65760234218853	36.0	36.0	36.0	32.0	36.0
32	34.6196909079066	36.0	36.0	36.0	32.0	36.0
33	34.59262992255557	36.0	36.0	36.0	32.0	36.0
34	34.550249748879814	36.0	36.0	36.0	32.0	36.0
35	34.48378774944891	36.0	36.0	36.0	32.0	36.0
36	34.435610384890964	36.0	36.0	36.0	32.0	36.0
37	34.38983966019068	36.0	36.0	36.0	32.0	36.0
38	34.32724302605822	36.0	36.0	36.0	32.0	36.0
39	34.258050416019586	36.0	36.0	36.0	32.0	36.0
40	34.20335935384119	36.0	36.0	36.0	32.0	36.0
41	34.18385768599178	36.0	36.0	36.0	32.0	36.0
42	34.08397698950609	36.0	36.0	36.0	32.0	36.0
43	34.05827873826249	36.0	36.0	36.0	32.0	36.0
44	33.988465612818956	36.0	36.0	36.0	32.0	36.0
45	33.86719599030488	36.0	36.0	36.0	32.0	36.0
46	33.82894001570144	36.0	36.0	36.0	32.0	36.0
47	33.74195986876614	36.0	36.0	36.0	27.0	36.0
48	33.6954109286133	36.0	36.0	36.0	27.0	36.0
49	33.64367502819746	36.0	36.0	36.0	27.0	36.0
50	33.065424950204495	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	4.0
20	3.0
21	18.0
22	67.0
23	203.0
24	564.0
25	1460.0
26	2769.0
27	5141.0
28	8298.0
29	12328.0
30	16864.0
31	22643.0
32	32228.0
33	49212.0
34	98033.0
35	333549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.975777170540375	17.90843667607237	11.82410077245626	25.291685380930996
2	14.753061448377055	21.033144549730537	39.011354442357	25.2024395595354
3	18.428621872996608	26.28516968182301	28.682381819241463	26.603826625938915
4	11.558385014381559	16.314926995162722	36.83204602098782	35.2946419694679
5	12.788788212264265	38.67919353566935	33.86419968939947	14.667818562666913
6	32.504778148221156	36.58270267490594	17.683005219537698	13.229513957335207
7	28.76145255705923	31.480068908182417	21.473983732869375	18.284494801888975
8	27.663330967832618	34.104178022784225	19.7469599887553	18.485531020627853
9	27.923651822139945	13.777983475607666	18.800267407178854	39.498097295073535
10	15.419333605312508	27.85055315764393	32.84834189839178	23.881771338651784
11	36.130452222027955	21.2300260890731	23.62089594196638	19.018625746932564
12	24.956889606538383	24.14593425279318	30.448450939858002	20.44872520081044
13	30.509973722237365	20.56093509752598	25.46697452616892	23.46211665406774
14	22.929322710256024	20.587983215172166	26.697509702014454	29.785184372557357
15	24.13564342687875	28.87291228467155	24.017018854792894	22.97442543365681
16	23.49275948603321	26.399764134772298	26.74481987850198	23.36265650069251
17	22.32381305002177	26.814664733126953	27.208572026068506	23.652950190782775
18	23.22843958696159	25.09976276346283	29.617027549607123	22.05477009996846
19	24.758907481495957	25.424161704257557	27.122008412954717	22.69492240129177
20	25.135588223194326	24.640545506904544	27.772959148691083	22.450907121210044
21	25.620258285251964	24.584237537410907	25.964284367468537	23.831219809868596
22	24.719825295773294	25.875663797211452	26.36676231517383	23.03774859184142
23	22.77288481910528	25.89898830603828	27.374361048925394	23.953765825931043
24	23.89035732773841	25.88800554007124	27.41409632730302	22.80754080488733
25	23.820396520928256	25.530620151052325	27.27924110433444	23.369742223684977
26	22.45783413416622	26.488683485585867	28.057893103808855	22.99558927643906
27	23.603285157649513	26.405885993167068	27.107007616340763	22.883821232842664
28	22.743074602303896	25.963384530992865	27.392347778387272	23.901193088315964
29	23.123021199202565	26.0204912482794	27.254011598108235	23.6024759544098
30	22.933458583462183	26.016771632217168	27.915236746742185	23.134533037578468
31	23.786854641983478	25.632608140378068	26.71516905264349	23.86536816499497
32	22.762421468548848	26.873912559032508	26.81700136278316	23.546664609635474
33	22.360580648257987	26.476305738598573	27.63719083634469	23.525922776798755
34	23.10813451589568	26.474803125310697	27.74313209888818	22.673930259905443
35	23.596052611279575	26.443630404561063	27.348026048514416	22.612290935644946
36	22.786313914598253	26.87983104106203	27.391713537800623	22.9421415065391
37	23.425471188706897	27.008305263428557	26.311485947905684	23.25473759995886
38	23.070975749409424	27.226627991894752	26.47078991850183	23.23160634019399
39	23.845040918550314	26.297582599930745	26.527460169982547	23.32991631153639
40	24.403426281298586	26.480485041302902	26.641959775306706	22.4741289020918
41	23.31072336471988	26.499431750978363	27.334749241906092	22.85509564239566
42	23.976687379472892	27.705335333190483	26.655753160488537	21.66222412684808
43	23.046635286212368	26.77354991103538	26.83783070324901	23.34198409950324
44	22.53423589124737	27.31414740674587	26.711623147216475	23.43999355479028
45	23.035532768689887	27.361380376668947	26.27734723857814	23.325739616063025
46	22.87184532921024	26.47943625178057	26.621710340240767	24.027008078768418
47	24.05685604969762	26.350605449733273	26.62350351750525	22.969034983063864
48	23.385118560915778	27.54267867274856	25.435786146380263	23.636416619955398
49	23.279612469274202	27.000647941500137	26.277798918726198	23.441940670499463
50	22.919616440798247	28.358457123854514	25.176300786111234	23.545625649236
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	39.0
2	29.0
3	120.5
4	212.0
5	228.0
6	244.0
7	243.0
8	242.0
9	319.0
10	396.0
11	634.5
12	873.0
13	1482.5
14	2092.0
15	2874.0
16	3656.0
17	3986.0
18	4316.0
19	4434.5
20	4553.0
21	4769.5
22	4986.0
23	5276.5
24	5567.0
25	5429.0
26	5291.0
27	6133.0
28	6975.0
29	8386.5
30	9798.0
31	11646.5
32	13495.0
33	16658.0
34	19821.0
35	22954.5
36	26088.0
37	27407.0
38	28726.0
39	28860.5
40	28995.0
41	30071.0
42	31147.0
43	31684.5
44	32222.0
45	35129.5
46	38037.0
47	40592.0
48	43147.0
49	43545.0
50	43943.0
51	41319.5
52	38696.0
53	37167.0
54	35638.0
55	35092.5
56	34547.0
57	33063.0
58	31579.0
59	28707.5
60	25836.0
61	22512.5
62	19189.0
63	16422.5
64	13656.0
65	11455.5
66	9255.0
67	8067.0
68	6879.0
69	5954.5
70	5030.0
71	3973.0
72	2916.0
73	2496.5
74	2077.0
75	1583.0
76	1089.0
77	946.5
78	804.0
79	670.0
80	536.0
81	434.5
82	333.0
83	272.5
84	212.0
85	160.0
86	108.0
87	75.0
88	42.0
89	30.0
90	18.0
91	16.0
92	14.0
93	14.5
94	15.0
95	10.5
96	6.0
97	5.5
98	5.0
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008913480954290984
2	3.4282619054965327E-4
3	0.0
4	0.0
5	0.0
6	1.7141309527482664E-4
7	1.7141309527482664E-4
8	0.0
9	0.0010284785716489597
10	0.001371304762198613
11	0.0
12	0.0
13	5.142392858244799E-4
14	3.4282619054965327E-4
15	0.005656632144069278
16	3.4282619054965327E-4
17	0.0
18	3.4282619054965327E-4
19	0.0
20	3.4282619054965327E-4
21	0.0
22	0.0
23	0.001371304762198613
24	0.0
25	0.0020569571432979194
26	0.005999458334618932
27	0.005999458334618932
28	0.004456740477145492
29	0.0025711964291223995
30	0.0034282619054965325
31	0.008056415477916851
32	0.0035996750007713588
33	0.006513697620443411
34	0.0034282619054965325
35	0.002228370238572746
36	0.008399241668466503
37	0.0035996750007713588
38	0.010284785716489598
39	0.005485219048794452
40	0.002228370238572746
41	0.0025711964291223995
42	0.001885544048023093
43	0.001371304762198613
44	0.0011998916669237864
45	0.0015427178574734396
46	8.570654763741331E-4
47	0.003085435714946879
48	0.0025711964291223995
49	0.0
50	0.001371304762198613
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	583386.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.28614977388332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.88788541703794	52.8085860222945
2	12.54683480127135	16.38269072047946
3	3.4298021670237997	6.717557339133159
4	1.2178376213033557	3.180317173787227
5	0.5857740462895034	1.9121466059855086
6	0.29599719197991525	1.1594710204949785
7	0.1915725361828676	0.8754923302858163
8	0.13993218947393687	0.7308507104146289
9	0.09044204667815468	0.5314151704757898
>10	0.5003399712320735	6.316940720666736
>50	0.06762093451034158	3.0502886349009426
>100	0.04411206817125389	5.425606996927221
>500	0.0015848647247141481	0.7220461666811298
>1k	2.6414412078569136E-4	0.18659038747288811
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1082	0.1854689690873624	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	896	0.15358613336624466	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	841	0.14415841312612918	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	790	0.13541634526711302	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	630	0.10799025002314078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.714130952748266E-4	0.0
13	0.0	0.0	0.0	5.142392858244799E-4	0.0
14	0.0	0.0	0.0	6.856523810993064E-4	0.0
15	0.0	0.0	0.0	8.570654763741331E-4	0.0
16	0.0	0.0	0.0	8.570654763741331E-4	0.0
17	0.0	0.0	0.0	0.0010284785716489597	0.0
18	0.0	0.0	0.0	0.0011998916669237864	0.0
19	0.0	0.0	0.0	0.0011998916669237864	0.0
20	0.0	0.0	0.0	0.0011998916669237864	0.0
21	0.0	0.0	0.0	0.0020569571432979194	0.0
22	0.0	0.0	0.0	0.0042853273818706655	0.0
23	0.0	0.0	0.0	0.006685110715718238	0.0
24	0.0	0.0	0.0	0.01062761190703925	0.0
25	0.0	0.0	0.0	0.013370221431436477	0.0
26	0.0	0.0	0.0	0.016284244051108527	0.0
27	0.0	0.0	0.0	0.019369679766055407	0.0
28	0.0	0.0	0.0	0.025711964291223993	0.0
29	0.0	0.0	0.0	0.03496827143606463	0.0
30	0.0	0.0	0.0	0.052966646439921425	0.0
31	0.0	0.0	0.0	0.08656361311378744	0.0
32	0.0	0.0	0.0	0.1263314512175472	0.0
33	0.0	0.0	0.0	0.15804287384339014	0.0
34	0.0	0.0	0.0	0.19215407980308064	0.0
35	0.0	0.0	0.0	0.23346463576431384	0.0
36	0.0	0.0	0.0	0.28951671791918215	0.0
37	0.0	0.0	0.0	0.37385196079439686	0.0
38	0.0	0.0	0.0	0.4816707977222628	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAAT	35	3.2153592E-4	31.429367	27
CGTAATT	35	3.2153592E-4	31.429367	28
TCCGTAA	45	0.0013964435	24.447159	26
CGCGTAA	55	1.5919658E-4	24.00061	10
TCGCGTA	55	1.5919658E-4	24.00061	9
GACGTGA	345	0.0	22.953169	7
CGTCGTA	70	3.211048E-5	22.000557	10
CGGTTGG	50	0.002579757	21.99867	40
TAATACC	70	3.2153013E-5	21.996786	4
TAGGACG	695	0.0	21.838535	4
TTAGGAC	455	0.0	21.755062	3
GTCCTAA	405	0.0	21.183905	1
CGACCAT	115	8.745701E-9	21.044012	10
AGGACGT	705	0.0	20.904747	5
ATAGCGT	95	6.863411E-7	20.83906	6
GCGTAAC	75	5.4653494E-5	20.533854	11
GATATAC	140	1.8371793E-10	20.427338	1
TTTAGGA	500	0.0	20.237043	2
GTCCTAC	625	0.0	20.062788	1
ACTATCC	55	0.0044846684	19.99708	8
>>END_MODULE