FastQCFastQC Report
Thu 2 Feb 2017
SRR4064076_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064076_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375384
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT129510.5452171101598731No Hit
GTCCTACAGTGGACATTTCTAAATT120570.5075810900469145No Hit
CTGTAGGACGTGGAATATGGCAAGA119290.5021924876146341No Hit
CTTTAGGACGTGAAATATGGCGAGG109070.4591678650693951No Hit
GTCCTACAGTGTGCATTTCTCATTT91710.3860849445815918No Hit
CTGTAGGACCTGGAATATGGCGAGA65470.2756185947198432No Hit
CTGAAGGACCTGGAATATGGCGAGA52760.22211145650555866No Hit
ATTTAGAAATGTCCACTGTAGGACG51320.2160492787692432No Hit
GTCCTTCAGTGTGCATTTCTCATTT44700.1881801005647929No Hit
GATATACACTGTTCTACAAATCCCG35860.15096506501685622No Hit
GAATATGGCAAGAAAACTGAAAATC35460.14928112675676858No Hit
TTTCTAAATTTTCCACCTTTTTCAG35290.14856545299623136No Hit
TTGTAGAACAGTGTATATCAATGAG34270.14427141043300787No Hit
CTGTAGGACATGGAATATGGCAAGA30620.12890547380970824No Hit
GGAATATGGCGAGAAAACTGAAAAT28860.12149614546532266No Hit
ATTCCAGGTCCTTCAGTGTGCATTT27690.11657062605456632No Hit
GTGTATATCAATGAGTTACAATGAG26460.11139251590479686No Hit
TTCCAGGTCCTTCAGTGTGCATTTC25370.10680378414605807No Hit
GTGTATATCAATGAGTTACAATGAA24810.10444627058193538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCG350.002167552716.289216
GGTATCA8950.015.1730671
CCGTCGT953.0559022E-1015.0013139
CCGACCA1400.014.9298789
TCCAACG6050.014.9199218
CCAACGA4200.014.70521919
TATCGCG400.005272694414.25305116
GGCGACA400.005273431514.25275119
TCGCGAG400.005274168714.2524518
TCGCGTA602.5672653E-514.2512479
GGCGAAA1101.8553692E-1013.82084819
TAGGACC26400.013.5997394
CGTCGTA1051.369699E-913.57261810
CGAACGA852.6940143E-713.41463616
CGAGCCG1650.013.24525915
GTATCAA29850.013.2345551
GTCGTAG1102.746674E-912.95513311
CCACCTT12350.012.77061813
TAGAAAT15850.012.7641844
TTCGGAA1352.7284841E-1112.66884318
Produced by FastQC (version 0.11.5)