##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064076_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2375384
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29448080815565	32.0	32.0	32.0	32.0	32.0
2	30.88826859152036	32.0	32.0	32.0	32.0	32.0
3	30.907566523980964	32.0	32.0	32.0	32.0	32.0
4	30.933153123873865	32.0	32.0	32.0	32.0	32.0
5	30.890127238374934	32.0	32.0	32.0	32.0	32.0
6	34.558189749531024	36.0	36.0	36.0	32.0	36.0
7	34.48710987360359	36.0	36.0	36.0	32.0	36.0
8	34.457455299858886	36.0	36.0	36.0	32.0	36.0
9	34.56918291947744	36.0	36.0	36.0	32.0	36.0
10	34.26403267850588	36.0	36.0	36.0	32.0	36.0
11	34.54034042495866	36.0	36.0	36.0	32.0	36.0
12	34.36317159667658	36.0	36.0	36.0	32.0	36.0
13	34.43206235286589	36.0	36.0	36.0	32.0	36.0
14	34.35203908083914	36.0	36.0	36.0	32.0	36.0
15	34.29807685831007	36.0	36.0	36.0	32.0	36.0
16	34.29704460415663	36.0	36.0	36.0	32.0	36.0
17	34.26438420061767	36.0	36.0	36.0	32.0	36.0
18	34.26089297561994	36.0	36.0	36.0	32.0	36.0
19	34.219947174856784	36.0	36.0	36.0	32.0	36.0
20	34.18727961458021	36.0	36.0	36.0	32.0	36.0
21	34.17142659881518	36.0	36.0	36.0	32.0	36.0
22	34.12453312811739	36.0	36.0	36.0	32.0	36.0
23	34.10001625000421	36.0	36.0	36.0	32.0	36.0
24	34.07624409358655	36.0	36.0	36.0	32.0	36.0
25	33.70557728771432	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	16.0
4	23.0
5	24.0
6	90.0
7	40.0
8	136.0
9	151.0
10	236.0
11	63.0
12	173.0
13	118.0
14	152.0
15	326.0
16	605.0
17	742.0
18	969.0
19	1337.0
20	2021.0
21	3009.0
22	4915.0
23	7537.0
24	11406.0
25	17156.0
26	24704.0
27	33077.0
28	44999.0
29	61853.0
30	81554.0
31	111287.0
32	156595.0
33	225599.0
34	491459.0
35	1093012.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36702304633441	19.75329699625258	12.173511666359225	25.706168291053782
2	12.587964609966551	20.599811808887083	45.92650442375418	20.885719157392185
3	18.69722303380673	25.736762027612208	29.899643248968772	25.666371689612287
4	10.54582364855506	17.482531614021187	40.47680894677747	31.49483579064628
5	11.146360050017472	40.00551539468754	37.05448452110796	11.793640034187026
6	30.008980829528863	39.95664949797037	18.804838111390072	11.229531561110692
7	27.164218572948673	31.830467808160627	22.828770431367825	18.17654318752287
8	22.842406577528237	42.06031646963646	20.229024189843038	14.868252762992265
9	26.600603239093196	14.694612841554337	20.58337541798589	38.121408501366574
10	14.151612596135296	29.827742062124734	35.91624761267701	20.104397729062956
11	34.28213995375031	21.271445239609616	27.51367904063486	16.932735766005212
12	24.69704494817949	24.18505333281098	34.0247368534126	17.093164865596936
13	30.18072306920437	22.97930832279842	26.01715438993063	20.822814218066576
14	19.10313055024754	24.667954071333003	27.826013325649345	28.402902052770113
15	20.606277862188975	36.93042977538384	22.588829386834913	19.87446297559227
16	19.406374708430032	28.33501910660055	32.524358756703954	19.734247428265466
17	19.450129030029494	30.854184692817537	29.922876859940708	19.77280941721226
18	19.454584062206134	27.660427852546928	34.735339123869615	18.149648961377327
19	23.87679088043889	26.148507998110905	27.399506998373376	22.575194123076834
20	24.020221434737675	28.518728083720347	28.89622632367693	18.564824157865047
21	22.175115238437503	27.08138039238156	26.446847495130726	24.296656874050214
22	23.080251438779207	31.385272630754063	26.973760711848126	18.560715218618604
23	20.098128645854178	30.8294166843978	29.150949159755037	19.921505509992983
24	23.36915306679609	27.377744410065592	30.181685507627385	19.07141701551093
25	21.307414956643075	29.68021793479314	29.70444321047678	19.307923898087004
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	62.0
1	62.0
2	246.0
3	430.0
4	430.0
5	430.0
6	978.0
7	1526.0
8	1526.0
9	1526.0
10	2568.5
11	3611.0
12	3611.0
13	3611.0
14	6440.0
15	9269.0
16	9269.0
17	9269.0
18	17050.5
19	24832.0
20	24832.0
21	24832.0
22	44357.0
23	63882.0
24	63882.0
25	63882.0
26	102764.0
27	141646.0
28	141646.0
29	141646.0
30	195356.5
31	249067.0
32	249067.0
33	249067.0
34	271973.0
35	294879.0
36	294879.0
37	294879.0
38	306463.5
39	318048.0
40	318048.0
41	318048.0
42	323134.0
43	328220.0
44	328220.0
45	328220.0
46	325041.0
47	321862.0
48	321862.0
49	321862.0
50	288461.5
51	255061.0
52	255061.0
53	255061.0
54	203546.0
55	152031.0
56	152031.0
57	152031.0
58	124222.0
59	96413.0
60	96413.0
61	96413.0
62	76081.5
63	55750.0
64	55750.0
65	55750.0
66	42804.0
67	29858.0
68	29858.0
69	29858.0
70	21543.0
71	13228.0
72	13228.0
73	13228.0
74	10223.5
75	7219.0
76	7219.0
77	7219.0
78	5734.0
79	4249.0
80	4249.0
81	4249.0
82	2938.0
83	1627.0
84	1627.0
85	1627.0
86	1197.0
87	767.0
88	767.0
89	767.0
90	620.5
91	474.0
92	474.0
93	474.0
94	398.0
95	322.0
96	322.0
97	322.0
98	686.5
99	1051.0
100	1051.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039993533677081264
2	0.0058516854538045214
3	0.0024417104771270667
4	0.003915156454703745
5	0.009009069691468833
6	0.013681998363212012
7	0.022859461880689606
8	0.03683614943941695
9	0.05056024625913116
10	0.05834846071203645
11	0.05531737184387872
12	0.06386335851382345
13	0.06445273690485412
14	0.06803110570754034
15	0.059906103602617514
16	0.06723123503399872
17	0.06390545697032564
18	0.07813473526806614
19	0.07413538190035801
20	0.07842942446358148
21	0.0769138800295026
22	0.07762955379003984
23	0.08289186085281369
24	0.07712437231201355
25	0.07704017539900918
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2375384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.650612824359285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.48382325188037	33.58597372155924
2	16.37011138216213	15.600916787260891
3	6.080713396041811	8.692491591920497
4	2.783275269808463	5.304990890610287
5	1.408336084835918	3.355403875254517
6	0.7798259100973677	2.2295509507471967
7	0.4766275692627078	1.5898117035046941
8	0.3238056344351892	1.2343629533453777
9	0.21953320156503356	0.9414802430880695
>10	0.9105719840612592	7.595264138698475
>50	0.07974735205131243	2.657415990036511
>100	0.06882438157994786	6.786035567354953
>500	0.00939692045005578	3.028101348488279
>1k	0.004698460225027808	4.273532854228412
>5k	3.546007717002119E-4	1.1036239025529224
>10k+	3.546007717002119E-4	2.0210434813496905
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	12951	0.5452171101598731	No Hit
GTCCTACAGTGGACATTTCTAAATT	12057	0.5075810900469145	No Hit
CTGTAGGACGTGGAATATGGCAAGA	11929	0.5021924876146341	No Hit
CTTTAGGACGTGAAATATGGCGAGG	10907	0.4591678650693951	No Hit
GTCCTACAGTGTGCATTTCTCATTT	9171	0.3860849445815918	No Hit
CTGTAGGACCTGGAATATGGCGAGA	6547	0.2756185947198432	No Hit
CTGAAGGACCTGGAATATGGCGAGA	5276	0.22211145650555866	No Hit
ATTTAGAAATGTCCACTGTAGGACG	5132	0.2160492787692432	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	4470	0.1881801005647929	No Hit
GATATACACTGTTCTACAAATCCCG	3586	0.15096506501685622	No Hit
GAATATGGCAAGAAAACTGAAAATC	3546	0.14928112675676858	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	3529	0.14856545299623136	No Hit
TTGTAGAACAGTGTATATCAATGAG	3427	0.14427141043300787	No Hit
CTGTAGGACATGGAATATGGCAAGA	3062	0.12890547380970824	No Hit
GGAATATGGCGAGAAAACTGAAAAT	2886	0.12149614546532266	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	2769	0.11657062605456632	No Hit
GTGTATATCAATGAGTTACAATGAG	2646	0.11139251590479686	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	2537	0.10680378414605807	No Hit
GTGTATATCAATGAGTTACAATGAA	2481	0.10444627058193538	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGCG	35	0.0021675527	16.2892	16
GGTATCA	895	0.0	15.173067	1
CCGTCGT	95	3.0559022E-10	15.001313	9
CCGACCA	140	0.0	14.929878	9
TCCAACG	605	0.0	14.91992	18
CCAACGA	420	0.0	14.705219	19
TATCGCG	40	0.0052726944	14.253051	16
GGCGACA	40	0.0052734315	14.252751	19
TCGCGAG	40	0.0052741687	14.25245	18
TCGCGTA	60	2.5672653E-5	14.251247	9
GGCGAAA	110	1.8553692E-10	13.820848	19
TAGGACC	2640	0.0	13.599739	4
CGTCGTA	105	1.369699E-9	13.572618	10
CGAACGA	85	2.6940143E-7	13.414636	16
CGAGCCG	165	0.0	13.245259	15
GTATCAA	2985	0.0	13.234555	1
GTCGTAG	110	2.746674E-9	12.955133	11
CCACCTT	1235	0.0	12.770618	13
TAGAAAT	1585	0.0	12.764184	4
TTCGGAA	135	2.7284841E-11	12.668843	18
>>END_MODULE