FastQCFastQC Report
Thu 2 Feb 2017
SRR4064076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375384
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA62230.26197869481313335No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA45810.19285302923653608No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA44540.18750652526075784No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC41140.17319305005001295No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA28180.11863345042317369No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA24130.10158357553978642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9900.029.3321
GTATCAA28850.028.7473931
TAGGACC32400.026.2086854
TAGTACG1102.0190782E-1023.998914
GGACCTG49450.023.1334296
AGGACCT52400.022.6287445
GTAGGAC83450.022.3544143
CTGTAGG83700.022.1829811
TGTAGGA86850.022.0867232
TTAGGAC46550.021.5968463
GTCCTAC73300.021.1586551
GACCTGG53550.021.1161987
CGATCGC956.8722693E-720.84203510
TAGGACG82800.020.829974
GATATAC21650.020.728851
AGGACGT81900.020.5216565
GGACGTG81050.020.3840226
GACCGGG654.934902E-420.3071987
GACGTGA44000.020.1495087
CTTTAGG48600.020.1431181
Produced by FastQC (version 0.11.5)