##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064076_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2375384
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3798888937536	32.0	32.0	32.0	32.0	32.0
2	31.457920487803236	32.0	32.0	32.0	32.0	32.0
3	31.521171313774953	32.0	32.0	32.0	32.0	32.0
4	31.59031550267241	32.0	32.0	32.0	32.0	32.0
5	31.57307071193542	32.0	32.0	32.0	32.0	32.0
6	35.15969333800346	36.0	36.0	36.0	36.0	36.0
7	35.177633595241865	36.0	36.0	36.0	36.0	36.0
8	35.11634371537402	36.0	36.0	36.0	36.0	36.0
9	35.21866443488716	36.0	36.0	36.0	36.0	36.0
10	35.10491693132563	36.0	36.0	36.0	36.0	36.0
11	35.22329694904066	36.0	36.0	36.0	36.0	36.0
12	35.15071584215436	36.0	36.0	36.0	36.0	36.0
13	35.183809438810734	36.0	36.0	36.0	36.0	36.0
14	35.13021684072975	36.0	36.0	36.0	36.0	36.0
15	35.111656473227065	36.0	36.0	36.0	36.0	36.0
16	35.136998060103124	36.0	36.0	36.0	36.0	36.0
17	35.128222636845244	36.0	36.0	36.0	36.0	36.0
18	35.12272668334888	36.0	36.0	36.0	36.0	36.0
19	35.09550834728196	36.0	36.0	36.0	36.0	36.0
20	35.0812984342742	36.0	36.0	36.0	36.0	36.0
21	35.0647735271434	36.0	36.0	36.0	36.0	36.0
22	35.02820259797995	36.0	36.0	36.0	36.0	36.0
23	35.015498125776716	36.0	36.0	36.0	36.0	36.0
24	34.99989306992048	36.0	36.0	36.0	36.0	36.0
25	34.955330169774655	36.0	36.0	36.0	36.0	36.0
26	34.91270127272054	36.0	36.0	36.0	36.0	36.0
27	34.865897471735096	36.0	36.0	36.0	36.0	36.0
28	34.81188388908909	36.0	36.0	36.0	32.0	36.0
29	34.78876215382439	36.0	36.0	36.0	32.0	36.0
30	34.747869397116425	36.0	36.0	36.0	32.0	36.0
31	34.74089662976596	36.0	36.0	36.0	32.0	36.0
32	34.71098567642116	36.0	36.0	36.0	32.0	36.0
33	34.748131670500435	36.0	36.0	36.0	32.0	36.0
34	34.73767862375094	36.0	36.0	36.0	32.0	36.0
35	34.71173334500864	36.0	36.0	36.0	32.0	36.0
36	34.647031806225854	36.0	36.0	36.0	32.0	36.0
37	34.572291048520995	36.0	36.0	36.0	32.0	36.0
38	34.53727271043334	36.0	36.0	36.0	32.0	36.0
39	34.4087768546054	36.0	36.0	36.0	32.0	36.0
40	34.482942126409874	36.0	36.0	36.0	32.0	36.0
41	34.47446012939382	36.0	36.0	36.0	32.0	36.0
42	34.46420326145162	36.0	36.0	36.0	32.0	36.0
43	34.4667009628759	36.0	36.0	36.0	32.0	36.0
44	34.37491622407156	36.0	36.0	36.0	32.0	36.0
45	34.35565618022181	36.0	36.0	36.0	32.0	36.0
46	34.231902715518835	36.0	36.0	36.0	32.0	36.0
47	34.25696308470546	36.0	36.0	36.0	32.0	36.0
48	34.21493619557933	36.0	36.0	36.0	32.0	36.0
49	34.17576989657251	36.0	36.0	36.0	32.0	36.0
50	33.71298745802784	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	2.0
19	3.0
20	7.0
21	63.0
22	192.0
23	588.0
24	1532.0
25	3683.0
26	7747.0
27	14890.0
28	25430.0
29	39978.0
30	58305.0
31	83054.0
32	120328.0
33	192324.0
34	398807.0
35	1428449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.52986255369062	20.592737735466166	12.701005649251298	26.176394061591914
2	12.130642219137794	21.357193165031344	45.1672221583726	21.34494245745826
3	18.103690706129395	26.34273705339627	30.252650953198067	25.300921287276267
4	10.390193753936206	18.227958090144583	40.45897421216949	30.922873943749728
5	10.834627159229834	40.50679805875597	36.68042724881535	11.978147533198841
6	30.216946432548237	40.12946986565091	18.484965978060764	11.168617723740084
7	27.120468572857515	31.919568339084687	22.915336179470845	18.044626908586952
8	22.947068768670665	42.13209316893605	20.039117885781835	14.881720176611445
9	26.378656504540793	14.589686766951985	20.55203514757367	38.479621580933554
10	14.048427144999373	30.178052860995503	35.60678330292394	20.16673669108119
11	34.194092407795964	21.28977883154892	27.558070610899122	16.958058149756
12	24.654655400544165	24.379415344317394	33.836761344811634	17.129167910326807
13	30.17969395951455	22.934849783528644	26.03331183494627	20.852144422010532
14	19.198839090031143	24.68360825098153	27.71786216762132	28.399690491366
15	20.558070569037646	36.98536305656865	22.47038362878485	19.986182745608854
16	19.561044117869937	28.548887665216782	32.01589314311406	19.874175073799215
17	19.531999878756444	31.12759873771988	29.517038087315566	19.823363296208107
18	19.60330557637094	27.803719825880492	34.48753462603878	18.10543997170979
19	24.029630577624502	26.22249707836712	27.44318392310464	22.304688420903737
20	24.2022071546027	28.63924969878326	28.711575395118054	18.44696775149598
21	22.32628492908936	26.98755232838143	26.57317722103037	24.11298552149884
22	23.232156539164258	31.12112588301661	27.058954777761873	18.587762800057252
23	20.1465458992618	30.876896813752836	29.00817348070718	19.968383806278194
24	23.513508393411566	27.43432089670052	29.95872230700416	19.093448402883755
25	21.496717949063378	29.663366653419466	29.384207087478696	19.45570831003846
26	18.54533987805068	31.212096079190015	30.65417562537654	19.588388417382767
27	20.026439377832602	30.283949655719727	29.211723422215393	20.477887544232278
28	19.113463666947474	30.801051998304207	27.800785504416513	22.284698830331802
29	21.04266951146916	28.311355999134435	27.305683514599682	23.340290974796723
30	19.421723328305195	28.877459216713348	29.479783387270402	22.221034067711052
31	23.21204532785905	27.188000478269874	28.34932057048839	21.25063362338268
32	20.50005241418913	30.335817078268906	28.662983499844863	20.5011470076971
33	19.251927878788134	30.53624437381882	30.688815924360885	19.523011823032157
34	20.61687792276901	30.194929853543695	30.278623980977688	18.9095682427096
35	19.875874193586412	30.75102764581962	29.874855388636202	19.49824277195777
36	18.788520521472975	29.71793894817057	31.09858348472441	20.394957045632044
37	19.783867664938885	29.402089164165936	27.572975922239852	23.24106724865533
38	20.603279108158027	31.391505372767732	27.658081212721857	20.347134306352384
39	22.87779240057829	27.62235789711218	28.489158779195435	21.0106909231141
40	23.822532522835765	27.57872481772014	29.54396671453368	19.054775944910414
41	23.158839544665557	26.698926329163914	30.5633919628983	19.57884216327223
42	21.146242396890724	30.726117985437057	29.59099818805192	18.5366414296203
43	19.18006257598874	30.706270060184764	30.409345646455765	19.704321717370732
44	18.645320777720524	30.670064899854843	29.352281261892976	21.33233306053166
45	19.244449776980705	30.79257626754738	28.93011781396886	21.03285614150306
46	20.157037315201617	27.66386049435054	29.466240681934813	22.71286150851303
47	22.961283048925164	27.70868363376726	28.825364936922764	20.504668380384803
48	19.679961605496477	29.092087869526047	28.57822459100592	22.649725933971556
49	22.041964848408817	28.05164166072724	28.37672799468209	21.529665496181856
50	19.8323782452087	31.60198000461406	27.47294291194385	21.092698838233385
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.5
2	5.0
3	20.0
4	35.0
5	45.0
6	55.0
7	76.5
8	98.0
9	145.0
10	192.0
11	314.5
12	437.0
13	829.5
14	1222.0
15	1730.5
16	2239.0
17	2765.5
18	3292.0
19	4114.5
20	4937.0
21	6764.5
22	8592.0
23	11643.5
24	14695.0
25	20654.0
26	26613.0
27	38677.0
28	50741.0
29	66013.0
30	81285.0
31	101412.0
32	121539.0
33	151730.0
34	181921.0
35	212772.0
36	243623.0
37	244624.5
38	245626.0
39	227726.5
40	209827.0
41	194250.5
42	178674.0
43	167595.0
44	156516.0
45	153235.0
46	149954.0
47	145694.5
48	141435.0
49	135639.0
50	129843.0
51	118583.0
52	107323.0
53	97223.0
54	87123.0
55	78012.0
56	68901.0
57	60149.5
58	51398.0
59	43388.5
60	35379.0
61	29613.0
62	23847.0
63	19606.0
64	15365.0
65	12638.5
66	9912.0
67	8726.5
68	7541.0
69	6666.0
70	5791.0
71	4417.5
72	3044.0
73	2410.5
74	1777.0
75	1503.5
76	1230.0
77	1071.0
78	912.0
79	866.5
80	821.0
81	663.5
82	506.0
83	503.5
84	501.0
85	411.5
86	322.0
87	240.5
88	159.0
89	112.0
90	65.0
91	49.0
92	33.0
93	24.0
94	15.0
95	12.5
96	10.0
97	9.0
98	8.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008503888213442543
2	4.6308302152409884E-4
3	5.051814780262896E-4
4	0.0
5	0.0
6	2.10492282510954E-4
7	4.20984565021908E-5
8	0.0
9	0.0011787567820613425
10	9.261660430481977E-4
11	0.0
12	1.262953695065724E-4
13	5.051814780262896E-4
14	2.525907390131448E-4
15	0.003915156454703745
16	8.41969130043816E-4
17	0.0
18	1.683938260087632E-4
19	0.0
20	7.577722170394345E-4
21	0.0
22	1.683938260087632E-4
23	7.998706735416253E-4
24	3.7888610851971724E-4
25	0.001683938260087632
26	0.005683291627795759
27	0.0058516854538045214
28	0.003999353367708126
29	0.0024417104771270667
30	0.003578368802686218
31	0.006567359214341765
32	0.0030731873246599285
33	0.004420337932730034
34	0.0028626950421489746
35	0.0021891197381139218
36	0.007956608278914062
37	0.003367876520175264
38	0.0069462453228614825
39	0.004125648737214699
40	0.0022312181946161124
41	0.00315738423766431
42	0.001683938260087632
43	0.0013471506080701056
44	0.0013471506080701056
45	0.0012208552385635334
46	0.00105246141255477
47	0.001768135173092014
48	0.0018523320860963954
49	6.31476847532862E-4
50	0.001515544434078869
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2375384.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.47480576632333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.06984310747538	39.98060547996593
2	15.664920473237883	17.380168411955466
3	5.800503897969376	9.653454812599577
4	2.596449453608798	5.761501164840976
5	1.3496216010533029	3.743499808823315
6	0.7329705004573609	2.439683768719014
7	0.4581912890037943	1.7792650932914766
8	0.29741950271317846	1.319943131530404
9	0.1926250341859557	0.9617252721477555
>10	0.7415472379089152	7.045050153912705
>50	0.05092279762856995	1.974877187548435
>100	0.03933870269028082	4.370871171947155
>500	0.0038151365319770477	1.456110431613657
>1k	0.0017549628047077606	1.869832040873706
>5k	7.630273063946786E-5	0.26341207023038604
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	6223	0.26197869481313335	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	4581	0.19285302923653608	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	4454	0.18750652526075784	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	4114	0.17319305005001295	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2818	0.11863345042317369	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	2413	0.10158357553978642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	4.20984565021908E-5	0.0
18	0.0	0.0	0.0	1.262953695065724E-4	0.0
19	0.0	0.0	0.0	1.262953695065724E-4	0.0
20	0.0	0.0	0.0	2.10492282510954E-4	0.0
21	0.0	0.0	0.0	2.525907390131448E-4	0.0
22	0.0	0.0	0.0	2.525907390131448E-4	0.0
23	0.0	0.0	0.0	3.367876520175264E-4	0.0
24	0.0	0.0	0.0	4.6308302152409884E-4	0.0
25	0.0	0.0	0.0	4.6308302152409884E-4	0.0
26	0.0	0.0	0.0	7.156737605372436E-4	0.0
27	0.0	0.0	0.0	9.261660430481977E-4	0.0
28	0.0	0.0	0.0	0.00210492282510954	0.0
29	0.0	0.0	0.0	0.004588731758738798	0.0
30	0.0	0.0	0.0	0.010145728017027983	0.0
31	0.0	0.0	0.0	0.027616587465437166	0.0
32	0.0	0.0	0.0	0.04891840645554572	0.0
33	0.0	0.0	0.0	0.06975714242413016	0.0
34	0.0	0.0	0.0	0.09211142282679348	0.0
35	0.0	0.0	0.0	0.12027529022675913	0.0
36	0.0	0.0	0.0	0.15909006712177906	0.0
37	0.0	0.0	0.0	0.2229113271791003	0.0
38	0.0	0.0	0.0	0.31013932905163966	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	990	0.0	29.332	1
GTATCAA	2885	0.0	28.747393	1
TAGGACC	3240	0.0	26.208685	4
TAGTACG	110	2.0190782E-10	23.99891	4
GGACCTG	4945	0.0	23.133429	6
AGGACCT	5240	0.0	22.628744	5
GTAGGAC	8345	0.0	22.354414	3
CTGTAGG	8370	0.0	22.182981	1
TGTAGGA	8685	0.0	22.086723	2
TTAGGAC	4655	0.0	21.596846	3
GTCCTAC	7330	0.0	21.158655	1
GACCTGG	5355	0.0	21.116198	7
CGATCGC	95	6.8722693E-7	20.842035	10
TAGGACG	8280	0.0	20.82997	4
GATATAC	2165	0.0	20.72885	1
AGGACGT	8190	0.0	20.521656	5
GGACGTG	8105	0.0	20.384022	6
GACCGGG	65	4.934902E-4	20.307198	7
GACGTGA	4400	0.0	20.149508	7
CTTTAGG	4860	0.0	20.143118	1
>>END_MODULE