FastQCFastQC Report
Thu 2 Feb 2017
SRR4064075_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064075_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144887
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84400.3934939229898824No Hit
TATCAACGCAGAGTACTTTTTTTTT62830.2929291846143876No Hit
GTACTTTTTTTTTTTTTTTTTTTTT50340.2346976787122119No Hit
GTACATGGGGTGGTATCAACGCAAA33970.15837664175315527No Hit
GAGTACTTTTTTTTTTTTTTTTTTT33930.15819015174225962No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30840.14378379840056843No Hit
GGTATCAACGCAGAGTACTTTTTTT28580.13324711278496257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA3700.012.58197710
CGTCGTA3300.012.09177110
CCGTCGT3300.011.8044219
CGCGCTA658.0176676E-411.69368616
GTCTTAA1302.6284397E-911.6893211
CAACGGA907.4504787E-611.61302214
TGTACCG907.4698564E-611.6103125
TAAACCT1501.7644197E-1011.3981534
GTATTAG3500.011.3970891
CGCCGGT3850.011.3506637
ACCGTCG3600.011.348568
CGAGCCG3700.011.2986315
ACCGAGT855.318418E-511.1777898
GGACCGT855.3247426E-511.1764846
ATACCGT3850.011.10390956
GTTTACG600.00587173311.08464059
CGTTATT1553.1650416E-1011.025842
AACGGAC951.3589732E-511.00129715
TTAGTCT1303.275636E-810.9597624
GTCCTAT4800.010.6847711
Produced by FastQC (version 0.11.5)