##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064075_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2144887
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23117348373131	32.0	32.0	32.0	32.0	32.0
2	30.87560137200701	32.0	32.0	32.0	32.0	32.0
3	30.899871182024974	32.0	32.0	32.0	32.0	32.0
4	30.918688956574403	32.0	32.0	32.0	32.0	32.0
5	30.844943812890843	32.0	32.0	32.0	32.0	32.0
6	34.49304042590589	36.0	36.0	36.0	32.0	36.0
7	34.41662847506652	36.0	36.0	36.0	32.0	36.0
8	34.370677336381824	36.0	36.0	36.0	32.0	36.0
9	34.49003793673047	36.0	36.0	36.0	32.0	36.0
10	34.22603801505627	36.0	36.0	36.0	32.0	36.0
11	34.49677628704915	36.0	36.0	36.0	32.0	36.0
12	34.31785450702065	36.0	36.0	36.0	32.0	36.0
13	34.3921386068357	36.0	36.0	36.0	32.0	36.0
14	34.3012391794999	36.0	36.0	36.0	32.0	36.0
15	34.25162397832613	36.0	36.0	36.0	32.0	36.0
16	34.250102219837224	36.0	36.0	36.0	32.0	36.0
17	34.173287916799346	36.0	36.0	36.0	32.0	36.0
18	34.16512664769753	36.0	36.0	36.0	32.0	36.0
19	34.18244504255935	36.0	36.0	36.0	32.0	36.0
20	34.14711357754511	36.0	36.0	36.0	32.0	36.0
21	34.125761869972635	36.0	36.0	36.0	32.0	36.0
22	34.12285355825272	36.0	36.0	36.0	32.0	36.0
23	34.07768101536352	36.0	36.0	36.0	32.0	36.0
24	34.07078787833578	36.0	36.0	36.0	32.0	36.0
25	33.67374271931342	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	18.0
5	13.0
6	91.0
7	35.0
8	123.0
9	133.0
10	191.0
11	62.0
12	121.0
13	110.0
14	263.0
15	395.0
16	650.0
17	854.0
18	1090.0
19	1472.0
20	2160.0
21	3278.0
22	4973.0
23	7631.0
24	11100.0
25	16331.0
26	23415.0
27	31059.0
28	41944.0
29	56903.0
30	74647.0
31	101493.0
32	142805.0
33	201843.0
34	436133.0
35	983545.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.61630246041522	17.27859504521543	11.436525596890771	25.66857689747858
2	16.457279696076892	20.113085283013945	37.78691532311646	25.642719697792703
3	18.58906815015675	24.744551139192822	28.56882432913786	28.09755638151257
4	12.422516627308184	15.5400815454946	36.138548079904325	35.89885374729289
5	14.29037692292987	36.98893416309625	33.5207247230277	15.199964190946183
6	33.35200021635555	35.814863066522804	17.36509718747115	13.468039529650502
7	29.75865325203726	30.802196392972476	21.1924408641011	18.246709490889167
8	28.14112537637538	32.39085580392288	19.76479103937437	19.703227780327367
9	27.7629368832069	13.994769226032966	18.778450554632762	39.46384333612737
10	15.731756980859576	26.785816746226004	31.732397000862534	25.750029272051883
11	36.5580357809258	21.43021077458166	22.924767879267364	19.086985565225177
12	24.862586994025765	23.983293041690885	29.031862276292745	22.1222576879906
13	29.3066673135831	19.855311541580086	25.587193293964738	25.250827850872078
14	23.71882797089522	19.564081462031847	25.96308343457303	30.7540071324999
15	24.76688834898918	27.07458639825938	23.086450507742086	25.07207474500935
16	25.193752157726294	25.94184169520308	24.086057122596177	24.778349024474448
17	23.559271507885747	25.880419920029894	25.59934426566011	24.960964306424245
18	24.210767464223775	25.26962519123967	26.622848539113207	23.896758805423346
19	25.23201360891447	25.03311480748621	26.163847261770513	23.571024321828805
20	25.589250253822264	24.241006106665075	25.50503165312948	24.664711986383182
21	26.6020463866299	24.151701356326022	25.21464664137844	24.031605615665637
22	25.767711869626158	24.16267429121727	25.524669987476972	24.544943851679594
23	24.161948155873	24.337203795339114	26.11845620125469	25.382391847533192
24	24.365145204983467	25.143637267957107	25.937526536851113	24.55369099020831
25	24.501208223666254	24.666892805201872	25.950143451773002	24.881755519358876
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	326.0
1	326.0
2	854.0
3	1382.0
4	1382.0
5	1382.0
6	3874.5
7	6367.0
8	6367.0
9	6367.0
10	6394.5
11	6422.0
12	6422.0
13	6422.0
14	6337.5
15	6253.0
16	6253.0
17	6253.0
18	10736.5
19	15220.0
20	15220.0
21	15220.0
22	24708.5
23	34197.0
24	34197.0
25	34197.0
26	49849.5
27	65502.0
28	65502.0
29	65502.0
30	81355.0
31	97208.0
32	97208.0
33	97208.0
34	117591.0
35	137974.0
36	137974.0
37	137974.0
38	157875.0
39	177776.0
40	177776.0
41	177776.0
42	203446.5
43	229117.0
44	229117.0
45	229117.0
46	255465.5
47	281814.0
48	281814.0
49	281814.0
50	287501.5
51	293189.0
52	293189.0
53	293189.0
54	268988.5
55	244788.0
56	244788.0
57	244788.0
58	222423.0
59	200058.0
60	200058.0
61	200058.0
62	175107.0
63	150156.0
64	150156.0
65	150156.0
66	123250.0
67	96344.0
68	96344.0
69	96344.0
70	72505.5
71	48667.0
72	48667.0
73	48667.0
74	37029.0
75	25391.0
76	25391.0
77	25391.0
78	20172.0
79	14953.0
80	14953.0
81	14953.0
82	10290.5
83	5628.0
84	5628.0
85	5628.0
86	4336.5
87	3045.0
88	3045.0
89	3045.0
90	2189.5
91	1334.0
92	1334.0
93	1334.0
94	932.5
95	531.0
96	531.0
97	531.0
98	888.0
99	1245.0
100	1245.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03813720722816633
2	0.006247415365005242
3	0.0016317875953371903
4	0.00335682019612222
5	0.00829880548485771
6	0.012541453232734406
7	0.02153959625845091
8	0.03501349954566371
9	0.04830091282198083
10	0.05613349327959935
11	0.05478144070060568
12	0.0628937561745677
13	0.06457216627262882
14	0.06802223147419888
15	0.059630180983893326
16	0.06653031138703344
17	0.06228766363915674
18	0.07618116945088482
19	0.07394328932013668
20	0.07716024200808713
21	0.0744561368500998
22	0.07622779195360875
23	0.08075017471782896
24	0.07650752696995226
25	0.0773001095162589
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2144887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.142365708397634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.67817329519983	33.07400485497012
2	15.142391794290107	13.974115597439472
3	5.593791582004634	7.743323306202419
4	2.554942868150193	4.715644327449943
5	1.4024383256761408	3.2355911053410673
6	0.8484269224299457	2.348905519896772
7	0.5394557237156471	1.7424234301022992
8	0.3599108542809243	1.3285710608521775
9	0.27301021696118993	1.133760354583699
>10	1.401280295560562	11.667473073795524
>50	0.1073050463570755	3.448751424936695
>100	0.0836333506843655	8.06571320960924
>500	0.01005821829496146	3.1726371188100613
>1k	0.004876711900587373	3.422881473384919
>5k	3.047944937867108E-4	0.9262041426256039
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8440	0.3934939229898824	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6283	0.2929291846143876	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5034	0.2346976787122119	No Hit
GTACATGGGGTGGTATCAACGCAAA	3397	0.15837664175315527	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3393	0.15819015174225962	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3084	0.14378379840056843	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2858	0.13324711278496257	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3311251361959862E-4
2	0.0	0.0	0.0	0.0	2.3311251361959862E-4
3	0.0	0.0	0.0	0.0	2.3311251361959862E-4
4	0.0	0.0	0.0	0.0	2.3311251361959862E-4
5	0.0	0.0	0.0	0.0	2.3311251361959862E-4
6	0.0	0.0	0.0	0.0	3.2635751906743806E-4
7	0.0	0.0	0.0	4.662250272391972E-5	3.2635751906743806E-4
8	0.0	0.0	0.0	4.662250272391972E-5	3.2635751906743806E-4
9	0.0	0.0	0.0	4.662250272391972E-5	3.2635751906743806E-4
10	0.0	0.0	0.0	9.324500544783944E-5	3.2635751906743806E-4
11	0.0	0.0	0.0	1.3986750817175916E-4	3.2635751906743806E-4
12	0.0	0.0	0.0	1.3986750817175916E-4	5.12847529963117E-4
13	0.0	0.0	0.0	1.3986750817175916E-4	5.594700326870366E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCCA	370	0.0	12.581977	10
CGTCGTA	330	0.0	12.091771	10
CCGTCGT	330	0.0	11.804421	9
CGCGCTA	65	8.0176676E-4	11.693686	16
GTCTTAA	130	2.6284397E-9	11.689321	1
CAACGGA	90	7.4504787E-6	11.613022	14
TGTACCG	90	7.4698564E-6	11.610312	5
TAAACCT	150	1.7644197E-10	11.398153	4
GTATTAG	350	0.0	11.397089	1
CGCCGGT	385	0.0	11.350663	7
ACCGTCG	360	0.0	11.34856	8
CGAGCCG	370	0.0	11.29863	15
ACCGAGT	85	5.318418E-5	11.177789	8
GGACCGT	85	5.3247426E-5	11.176484	6
ATACCGT	385	0.0	11.1039095	6
GTTTACG	60	0.005871733	11.0846405	9
CGTTATT	155	3.1650416E-10	11.02584	2
AACGGAC	95	1.3589732E-5	11.001297	15
TTAGTCT	130	3.275636E-8	10.959762	4
GTCCTAT	480	0.0	10.684771	1
>>END_MODULE