FastQCFastQC Report
Thu 2 Feb 2017
SRR4064075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144887
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24590.11464473419811859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG1259.968062E-1021.12088426
GTATCAA61500.018.1711
GTATAAT2900.017.4469721
AACGCAG65050.017.1455296
TTAACGG1854.2200554E-1016.65089835
CGCGGTT7500.016.13363310
CGCAATA5500.016.00216336
ATCGATT1805.2641553E-915.89066529
CGTTTAT4450.015.82310131
GTATTAC2403.6379788E-1215.5821691
TAGTACA2403.6379788E-1215.5821694
ATACGAA6050.014.90867240
GTATTAG5200.014.8065861
CTAGCGG6100.014.78853929
CAATACG6250.014.78530838
GTACATG82450.014.6744651
TAGCGGC6450.014.669332530
CGTGCAA1655.422844E-714.6665969
CCGTCGT4800.014.6665959
ACCGTCG5100.014.665578
Produced by FastQC (version 0.11.5)