##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064075_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2144887
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.271067893087142	32.0	32.0	32.0	32.0	32.0
2	31.397371982766458	32.0	32.0	32.0	32.0	32.0
3	31.482396974759045	32.0	32.0	32.0	32.0	32.0
4	31.567364621073278	32.0	32.0	32.0	32.0	32.0
5	31.508093899585386	32.0	32.0	32.0	32.0	32.0
6	35.09491688839552	36.0	36.0	36.0	36.0	36.0
7	35.1136516748901	36.0	36.0	36.0	36.0	36.0
8	35.065681781837455	36.0	36.0	36.0	36.0	36.0
9	35.17654822841483	36.0	36.0	36.0	36.0	36.0
10	35.02692496154809	36.0	36.0	36.0	36.0	36.0
11	35.17983324995676	36.0	36.0	36.0	36.0	36.0
12	35.08470656029898	36.0	36.0	36.0	36.0	36.0
13	35.12553994685967	36.0	36.0	36.0	36.0	36.0
14	35.07639283561325	36.0	36.0	36.0	36.0	36.0
15	35.04147397974812	36.0	36.0	36.0	36.0	36.0
16	35.05673352488966	36.0	36.0	36.0	36.0	36.0
17	35.01469214928339	36.0	36.0	36.0	36.0	36.0
18	35.015084244531295	36.0	36.0	36.0	36.0	36.0
19	35.01293867695594	36.0	36.0	36.0	36.0	36.0
20	35.00225606290681	36.0	36.0	36.0	36.0	36.0
21	35.00398016305754	36.0	36.0	36.0	36.0	36.0
22	34.99407521235384	36.0	36.0	36.0	36.0	36.0
23	34.945411110235646	36.0	36.0	36.0	36.0	36.0
24	34.91845491161073	36.0	36.0	36.0	32.0	36.0
25	34.89542432771517	36.0	36.0	36.0	32.0	36.0
26	34.82810469735702	36.0	36.0	36.0	32.0	36.0
27	34.826488761412605	36.0	36.0	36.0	32.0	36.0
28	34.810390477447065	36.0	36.0	36.0	32.0	36.0
29	34.77572291687161	36.0	36.0	36.0	32.0	36.0
30	34.7554505202372	36.0	36.0	36.0	32.0	36.0
31	34.740194238670846	36.0	36.0	36.0	32.0	36.0
32	34.70135163297647	36.0	36.0	36.0	32.0	36.0
33	34.66227311741831	36.0	36.0	36.0	32.0	36.0
34	34.64841178113346	36.0	36.0	36.0	32.0	36.0
35	34.60368961162057	36.0	36.0	36.0	32.0	36.0
36	34.57088881605418	36.0	36.0	36.0	32.0	36.0
37	34.56494444695688	36.0	36.0	36.0	32.0	36.0
38	34.51788649005752	36.0	36.0	36.0	32.0	36.0
39	34.51213840169669	36.0	36.0	36.0	32.0	36.0
40	34.48123467576614	36.0	36.0	36.0	32.0	36.0
41	34.461486782287366	36.0	36.0	36.0	32.0	36.0
42	34.38727821092673	36.0	36.0	36.0	32.0	36.0
43	34.36981155650624	36.0	36.0	36.0	32.0	36.0
44	34.29535168985592	36.0	36.0	36.0	32.0	36.0
45	34.257194434951586	36.0	36.0	36.0	32.0	36.0
46	34.213295618836796	36.0	36.0	36.0	32.0	36.0
47	34.15761016780837	36.0	36.0	36.0	32.0	36.0
48	34.10330101306036	36.0	36.0	36.0	32.0	36.0
49	34.069527672087155	36.0	36.0	36.0	32.0	36.0
50	33.47622462162342	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	6.0
20	18.0
21	67.0
22	217.0
23	716.0
24	1791.0
25	4084.0
26	8290.0
27	15315.0
28	25361.0
29	38185.0
30	54945.0
31	78248.0
32	112217.0
33	178858.0
34	369305.0
35	1257261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75863171921932	18.225901358974145	12.007986176193846	26.00748074561269
2	15.756933494585706	20.85978782942433	37.294149131092766	26.089129544897194
3	17.905294203762086	25.286473704282862	28.889109570072648	27.919122521882407
4	12.104274024692211	16.190549898432877	36.20325919267542	35.50191688419949
5	13.888377336428444	37.415211150983716	33.36763195450389	15.328779558083946
6	33.33100220384981	36.26887806933991	17.031744855080674	13.3683748717296
7	29.633164310983716	30.95873716247592	21.228840347543272	18.17925817899709
8	28.087074050987304	32.7363166451193	19.55408373494734	19.622525568946056
9	27.74554050147795	13.900487677517415	18.649702078457338	39.7042697425473
10	15.633244982784463	27.06114370834528	31.770997242250676	25.534614066619575
11	36.77718220120687	21.370030216044015	22.868384208585347	18.984403374163765
12	24.779382008537524	24.060695123838865	29.00096228980215	22.158960577821457
13	29.458505332238012	19.859451213542215	25.569193212854064	25.11285024136571
14	23.734825505552287	19.524663827660447	26.03411283231432	30.70639783447294
15	24.776271802833843	27.057147786030335	23.141239934912043	25.02534047622378
16	25.212962121159137	26.0251810720022	24.086364409881273	24.675492396957384
17	23.584645717932926	25.978524742795305	25.626757959743333	24.81007157952843
18	24.26825743165119	25.07221849240983	26.761870127932564	23.897653948006415
19	25.241294875345353	24.979043175255004	26.347274400597513	23.43238754880213
20	25.716231885274222	24.082893563295883	25.69030958038968	24.510564971040218
21	26.673852748419847	24.06280610586945	25.41411272481954	23.84922842089117
22	25.82868001904063	24.053947830351902	25.710445352132773	24.4069267984747
23	24.203985875160967	24.125426481928542	26.404021867951215	25.266565774959275
24	24.486358211181976	24.970814218044833	26.107241430756034	24.435586140017158
25	24.659965041893315	24.464753528563495	26.214057560121834	24.661223869421352
26	24.394027345525753	25.176440419158187	26.381974799221364	24.047557436094696
27	24.862536874806796	24.93806990722026	25.68943707841961	24.50995613955334
28	24.198467267531612	24.78462996731159	26.52824486419927	24.488657900957527
29	24.23515095566792	25.0310629955218	26.23008296675502	24.503703082055264
30	24.246108972093513	25.086324823783595	26.328301953649884	24.339264250473004
31	24.566000716182746	25.040471785383904	25.589824982841453	24.803702515591894
32	24.4681843062035	25.119986646910558	25.346858620435636	25.064970426450305
33	24.15480920671658	24.797671744727438	25.893587320296422	25.15393172825956
34	24.86815952988283	24.881540562478932	25.93244117124598	24.31785873639226
35	25.297914192693217	24.74159944685567	25.924584197621346	24.035902162829764
36	24.403804127619278	25.349800417116803	25.6228426701257	24.62355278513822
37	25.114462033106804	25.30715921552245	25.491883686059786	24.086495065310963
38	24.357933039090526	25.12479519413777	25.919120218360113	24.598151548411586
39	24.82662702967832	25.155888806311832	25.302615910682498	24.714868253327353
40	24.987761290617364	25.353850228036297	25.654293059509502	24.00409542183684
41	24.102664045158082	25.738837879711173	25.907896276658988	24.25060179847176
42	25.273668304550313	25.696963084909324	25.4864579705442	23.54291063999616
43	24.60125491059065	24.931254062048048	25.606451534696532	24.86103949266477
44	24.291406994152037	25.50813600397977	25.270869884080415	24.92958711778778
45	24.450661909854936	25.56430414377855	25.335337510152208	24.649696436214306
46	24.31484974762898	25.448071620592135	25.245167745370555	24.99191088640833
47	24.53209687458476	25.310198686612257	25.55604510370262	24.601659335100372
48	25.186050653520116	25.83775245402234	24.767046212175607	24.20915068028194
49	24.50492452940148	25.864957165335976	24.825689142723935	24.80442916253861
50	24.51083054371847	25.95330231343771	24.92447059481738	24.611396548026445
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	72.0
1	73.5
2	75.0
3	185.0
4	295.0
5	380.5
6	466.0
7	492.0
8	518.0
9	735.5
10	953.0
11	1439.5
12	1926.0
13	3416.5
14	4907.0
15	7057.0
16	9207.0
17	10054.0
18	10901.0
19	10867.5
20	10834.0
21	11388.5
22	11943.0
23	13008.5
24	14074.0
25	14572.5
26	15071.0
27	17817.5
28	20564.0
29	23832.0
30	27100.0
31	31815.0
32	36530.0
33	41973.5
34	47417.0
35	53784.0
36	60151.0
37	69880.0
38	79609.0
39	87181.5
40	94754.0
41	103472.0
42	112190.0
43	116502.5
44	120815.0
45	131617.5
46	142420.0
47	150237.5
48	158055.0
49	162393.0
50	166731.0
51	162247.0
52	157763.0
53	154355.5
54	150948.0
55	150189.0
56	149430.0
57	145440.5
58	141451.0
59	127710.5
60	113970.0
61	99716.0
62	85462.0
63	73752.5
64	62043.0
65	52323.0
66	42603.0
67	36584.5
68	30566.0
69	27465.5
70	24365.0
71	18866.5
72	13368.0
73	11424.5
74	9481.0
75	7519.0
76	5557.0
77	4717.5
78	3878.0
79	3199.5
80	2521.0
81	2070.5
82	1620.0
83	1375.5
84	1131.0
85	852.0
86	573.0
87	401.5
88	230.0
89	171.5
90	113.0
91	98.0
92	83.0
93	64.0
94	45.0
95	47.5
96	50.0
97	41.0
98	32.0
99	29.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00843867299302947
2	4.196025245152775E-4
3	6.060925354109564E-4
4	0.0
5	0.0
6	1.8649001089567888E-4
7	1.3986750817175916E-4
8	0.0
9	0.0012588075735458324
10	0.001025695059926234
11	0.0
12	1.3986750817175916E-4
13	4.196025245152775E-4
14	2.3311251361959862E-4
15	0.004522382764220213
16	0.001025695059926234
17	0.0
18	3.2635751906743806E-4
19	4.662250272391972E-5
20	7.459600435827155E-4
21	0.0
22	0.0
23	6.060925354109564E-4
24	3.2635751906743806E-4
25	0.0015851650926132705
26	0.005221720305079009
27	0.006060925354109564
28	0.004196025245152776
29	0.0024243701416438255
30	0.003776422720637498
31	0.008531917998477308
32	0.003030462677054782
33	0.004895362786011571
34	0.0027973501634351835
35	0.002704105157987344
36	0.007646090446722835
37	0.0033101976933983005
38	0.008065692971238112
39	0.004149402742428855
40	0.0024709926443677453
41	0.0030770851797787013
42	0.0021446351253003072
43	0.0015385425898893506
44	0.0017716551035089493
45	0.0015385425898893506
46	0.001352052578993672
47	0.0024243701416438255
48	0.0019115226116807088
49	5.594700326870366E-4
50	0.0012588075735458324
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2144887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.48455750652622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79525438749734	36.02240951940317
2	14.971505222128206	14.817166222473215
3	5.282762251483809	7.842454572805696
4	2.407364984529429	4.765095640645765
5	1.2875746397901815	3.1857530653321016
6	0.7443715405216731	2.210093778189975
7	0.4858693919396208	1.683012230626797
8	0.3412171991506425	1.3507985690868622
9	0.22999759020820484	1.0243196081118329
>10	1.2789354553924506	11.376343985623324
>50	0.09309725333142636	3.218594036909224
>100	0.07037243858126167	7.258512027448391
>500	0.008164857791318949	2.7843136312640837
>1k	0.003512787654404658	2.461133112079644
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2459	0.11464473419811859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8649001089567888E-4
2	0.0	0.0	0.0	0.0	1.8649001089567888E-4
3	0.0	0.0	0.0	0.0	1.8649001089567888E-4
4	0.0	0.0	0.0	0.0	1.8649001089567888E-4
5	0.0	0.0	0.0	0.0	2.3311251361959862E-4
6	0.0	0.0	0.0	0.0	2.797350163435183E-4
7	0.0	0.0	0.0	0.0	2.797350163435183E-4
8	0.0	0.0	0.0	0.0	3.2635751906743806E-4
9	0.0	0.0	0.0	0.0	3.2635751906743806E-4
10	0.0	0.0	0.0	0.0	3.2635751906743806E-4
11	0.0	0.0	0.0	4.662250272391972E-5	3.2635751906743806E-4
12	0.0	0.0	0.0	4.662250272391972E-5	5.12847529963117E-4
13	0.0	0.0	0.0	4.662250272391972E-5	6.993375408587959E-4
14	0.0	0.0	0.0	4.662250272391972E-5	6.993375408587959E-4
15	0.0	0.0	0.0	9.324500544783944E-5	9.324500544783945E-4
16	0.0	0.0	0.0	9.324500544783944E-5	0.001025695059926234
17	0.0	0.0	0.0	9.324500544783944E-5	0.001025695059926234
18	0.0	0.0	0.0	1.3986750817175916E-4	0.001165562568097993
19	0.0	0.0	0.0	1.3986750817175916E-4	0.001165562568097993
20	0.0	0.0	0.0	1.8649001089567888E-4	0.0012588075735458324
21	0.0	0.0	0.0	3.2635751906743806E-4	0.0012588075735458324
22	0.0	0.0	0.0	6.060925354109564E-4	0.0013054300762697522
23	0.0	0.0	0.0	0.0011189400653740733	0.0013520525789936719
24	0.0	0.0	0.0	0.0018182776062328691	0.0013986750817175917
25	0.0	0.0	0.0	0.002331125136195986	0.0013986750817175917
26	0.0	0.0	0.0	0.002983840174330862	0.001491920087165431
27	0.0	0.0	0.0	0.0033101976933983	0.0016317875953371903
28	0.0	0.0	0.0	0.005641322829594286	0.00167841009806111
29	0.0	0.0	0.0	0.010769798129225456	0.00167841009806111
30	0.0	0.0	0.0	0.019394961133150604	0.00167841009806111
31	0.0	0.0	0.0	0.04270621249511047	0.00167841009806111
32	0.0	0.0	0.0	0.06890805902595334	0.00167841009806111
33	0.0	0.0	0.0	0.09091388031164346	0.00167841009806111
34	0.0	0.0	0.0	0.11520420423080563	0.0018182776062328691
35	0.0	0.0	0.0	0.14504260597411425	0.001864900108956789
36	0.0	0.0	0.0	0.18751570595560513	0.001864900108956789
37	0.0	0.0	0.0	0.24779860197763331	0.001864900108956789
38	0.0	0.0	0.0	0.32640414157016195	0.0019115226116807086
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATCG	125	9.968062E-10	21.120884	26
GTATCAA	6150	0.0	18.171	1
GTATAAT	290	0.0	17.446972	1
AACGCAG	6505	0.0	17.145529	6
TTAACGG	185	4.2200554E-10	16.650898	35
CGCGGTT	750	0.0	16.133633	10
CGCAATA	550	0.0	16.002163	36
ATCGATT	180	5.2641553E-9	15.890665	29
CGTTTAT	445	0.0	15.823101	31
GTATTAC	240	3.6379788E-12	15.582169	1
TAGTACA	240	3.6379788E-12	15.582169	4
ATACGAA	605	0.0	14.908672	40
GTATTAG	520	0.0	14.806586	1
CTAGCGG	610	0.0	14.788539	29
CAATACG	625	0.0	14.785308	38
GTACATG	8245	0.0	14.674465	1
TAGCGGC	645	0.0	14.6693325	30
CGTGCAA	165	5.422844E-7	14.666596	9
CCGTCGT	480	0.0	14.666595	9
ACCGTCG	510	0.0	14.66557	8
>>END_MODULE