FastQCFastQC Report
Thu 2 Feb 2017
SRR4064074_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064074_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550436
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT53120.9650531578603143No Hit
CTGTAGGACGTGGAATATGGCAAGA43380.7881025223640895No Hit
GTCCTACAGTGGACATTTCTAAATT42980.7808355558139366No Hit
CTTTAGGACGTGAAATATGGCGAGG41660.7568545661984317No Hit
GTATCAACGCAGAGTACTTTTTTTT27360.49706051203046314No Hit
GTCCTACAGTGTGCATTTCTCATTT24680.44837183614443826No Hit
ATTTAGAAATGTCCACTGTAGGACG23110.41984899243508783No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21160.3844225305030921No Hit
TATCAACGCAGAGTACTTTTTTTTT20950.3806073730642618No Hit
CTGAAGGACCTGGAATATGGCGAGA20790.37770058644420057No Hit
GTCCTTCAGTGTGCATTTCTCATTT19420.35281122600992665No Hit
TTTCTAAATTTTCCACCTTTTTCAG17190.31229788749282383No Hit
CTGTAGGACCTGGAATATGGCGAGA16460.29903567353879473No Hit
GATATACACTGTTCTACAAATCCCG14540.26415423409806044No Hit
GAATATGGCAAGAAAACTGAAAATC13480.24489677274015506No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13340.24235333444760152No Hit
TTGTAGAACAGTGTATATCAATGAG12890.23417799707867942No Hit
GGAATATGGCGAGAAAACTGAAAAT11650.21165040077320524No Hit
GTACATGGGGTGGTATCAACGCAAA11190.2032933892405293No Hit
GCCATATTCCACGTCCTACAGTGGA10650.1934829843978228No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10550.19166624276028457No Hit
CCATATTCCAGGTCCTTCAGTGTGC10240.18603434368391603No Hit
ATTCCAGGTCCTTCAGTGTGCATTT10110.18367257955511632No Hit
GGTATCAACGCAGAGTACTTTTTTT10100.18349090539136248No Hit
GTTCTACAGTGTGGTTTTTATCATT9550.17349882638490213No Hit
GAAATATGGCGAGGAAAACTGAAAA9510.17277212972988684No Hit
TTCCAGGTCCTTCAGTGTGCATTTC9180.16677688232601065No Hit
GTCCACTGTAGGACGTGGAATATGG8970.16296172488718036No Hit
GTGTATATCAATGAGTTACAATGAG8910.1618716799046574No Hit
CTGTAGGACATGGAATATGGCAAGA8760.15914656744835004No Hit
GTGTATATCAATGAGTTACAATGAA8440.15333299420822766No Hit
GACCTGGAATATGGCGAGAAAACTG8420.15296964588072No Hit
CCATATTTCACGTCCTAAAGTGTGT8360.15187960089819708No Hit
ACCTGGAATATGGCGAGAAAACTGA8120.14751942096810527No Hit
ACAGTGGACATTTCTAAATTTTCCA8120.14751942096810527No Hit
CCACTGTAGGACGTGGAATATGGCA8110.14733774680435147No Hit
CCTAAAGTGTGTATTTCTCATTTTC7780.14134249940047525No Hit
ATACACACTTTAGGACGTGAAATAT7690.13970743192669083No Hit
GTATCAACGCAGAGTACATGGGGTG7550.13716399363413728No Hit
CACTTTAGGACGTGAAATATGGCGA7420.13480222950533757No Hit
GTTCTACAAATCCCGTTTCCAACGA7370.13389385868656847No Hit
GTGTATTTCTCATTTTCCGTGATTT7340.133348836195307No Hit
GAAATACACACTTTAGGACGTGAAA7090.12880698210146138No Hit
GATATACACTGTTCTACAATGCCGG6970.1266268921364155No Hit
AGTGTGTATTTCTCATTTTCCGTGA6720.12208503804256989No Hit
CTGTAGAACATATTAGATGAGTGAG6250.11354635234614016No Hit
GTACATGGGTGGTATCAACGCAAAA6140.11154793654484808No Hit
CTACAGTGGACATTTCTAAATTTTC6050.10991286907106367No Hit
GTAGGACGTGGAATATGGCAAGAAA5840.10609771163223336No Hit
ATTCCACGTCCTACAGTGGACATTT5680.10319092501217217No Hit
TCCTAAAGTGTGTATTTCTCATTTT5680.10319092501217217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT401.285341E-518.9955461
TTGCGAG350.002163300516.29078118
ATTGCGA508.6745735E-515.2047317
TAAGACC508.708291E-515.1978194
TCCTATA1004.0017767E-1115.1936742
CCAACGT704.405283E-714.93321619
TATTGCG456.7365874E-414.78237616
CCACCTT5600.014.58984413
TAGAAAT5350.014.3811124
TATATTG400.005278391814.2479535
TTCCTAT400.00528157714.2466591
TAGGGCA551.9589142E-413.8161974
CACCTTT5950.013.57318314
ACCTTTT6000.013.46129615
CAAGGGC500.001499020413.2980924
TAAGGCT500.001499020413.2980924
AAATGTC5500.013.298097
TCCAACG2300.013.22150418
GGACGAG655.447334E-513.15195756
CTCTTAT655.451984E-513.15076351
Produced by FastQC (version 0.11.5)