##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064074_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 550436 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.274551446489692 32.0 32.0 32.0 32.0 32.0 2 31.02156290649594 32.0 32.0 32.0 32.0 32.0 3 31.040008284341866 32.0 32.0 32.0 32.0 32.0 4 31.045992631295917 32.0 32.0 32.0 32.0 32.0 5 31.065795115145086 32.0 32.0 32.0 32.0 32.0 6 34.70472679839255 36.0 36.0 36.0 32.0 36.0 7 34.62123843643948 36.0 36.0 36.0 32.0 36.0 8 34.58804111649674 36.0 36.0 36.0 32.0 36.0 9 34.62708471102908 36.0 36.0 36.0 32.0 36.0 10 34.437420517553356 36.0 36.0 36.0 32.0 36.0 11 34.6397274160847 36.0 36.0 36.0 32.0 36.0 12 34.52493114549194 36.0 36.0 36.0 32.0 36.0 13 34.54757501326221 36.0 36.0 36.0 32.0 36.0 14 34.503141146291306 36.0 36.0 36.0 32.0 36.0 15 34.489390228836776 36.0 36.0 36.0 32.0 36.0 16 34.47166428067931 36.0 36.0 36.0 32.0 36.0 17 34.44299609763896 36.0 36.0 36.0 32.0 36.0 18 34.45527182088381 36.0 36.0 36.0 32.0 36.0 19 34.34910325632771 36.0 36.0 36.0 32.0 36.0 20 34.311485803980844 36.0 36.0 36.0 32.0 36.0 21 34.27746186659303 36.0 36.0 36.0 32.0 36.0 22 34.235814881294104 36.0 36.0 36.0 32.0 36.0 23 34.2683036719982 36.0 36.0 36.0 32.0 36.0 24 34.221346350892745 36.0 36.0 36.0 32.0 36.0 25 33.89047591363937 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 9.0 6 23.0 7 6.0 8 29.0 9 32.0 10 52.0 11 10.0 12 31.0 13 38.0 14 68.0 15 135.0 16 186.0 17 242.0 18 267.0 19 379.0 20 495.0 21 750.0 22 1113.0 23 1666.0 24 2362.0 25 3465.0 26 4918.0 27 6586.0 28 9267.0 29 12529.0 30 16814.0 31 23281.0 32 32888.0 33 47559.0 34 110453.0 35 274777.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.59642924644888 19.726377924136028 12.459472819528647 25.217720009886452 2 11.334806196198414 21.103120991566165 48.68695971308244 18.875113099152983 3 19.079724284276427 27.124460868206572 30.334361635254403 23.461453212262594 4 10.187367032631622 17.741821201016684 40.59631152380832 31.474500242543378 5 9.865693282120418 41.56622127903892 37.119491553139454 11.448593885701204 6 28.324472434306397 40.08100182063312 19.88578343550925 11.708742309551237 7 25.771146448319787 32.47709956261573 23.109407850384414 18.642346138680068 8 21.976046815934865 43.87285548124455 20.35493602791509 13.796161674905496 9 27.358185354150383 14.288518511786839 20.975117686617352 37.37817844744543 10 15.14506492066464 29.727538613389854 36.25468750284022 18.872708963105286 11 33.36053877684569 20.118879911294503 29.918111009115943 16.60247030274387 12 26.25976901583585 23.8088572410245 34.97466736716956 14.956706375970086 13 31.13171361310319 23.440314854706916 26.56832001745153 18.859651514738363 14 18.3895592039602 26.086719399061582 28.48476474940599 27.03895664757223 15 19.312405924827118 40.03312173236477 23.230659598758027 17.423812744050085 16 17.019652044285273 27.683749340992964 36.11631246932209 19.180286145399677 17 17.424571570885544 31.80401060915376 31.882543070146575 18.88887474981412 18 17.50927698598407 27.07225413032688 39.184425208675364 16.23404367501368 19 24.03392511385639 24.84569163780487 28.651267646604307 22.469115601734437 20 23.757774985863662 29.12049250819544 30.90376199316001 16.217970512780884 21 21.093271323354283 26.262729105721803 27.69758862875646 24.946410942167454 22 22.55249364112876 32.98849676102098 28.06484866886417 16.39416092898609 23 18.35537631866993 32.45155331226613 31.264795831166616 17.92827453789733 24 23.417295748425477 27.40923768381551 32.49443644456081 16.679030123198206 25 20.289301726865133 30.332864254364434 32.42081110662638 16.957022912144055 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 357.0 1 357.0 2 459.5 3 562.0 4 562.0 5 562.0 6 1613.5 7 2665.0 8 2665.0 9 2665.0 10 2481.0 11 2297.0 12 2297.0 13 2297.0 14 2130.5 15 1964.0 16 1964.0 17 1964.0 18 3850.5 19 5737.0 20 5737.0 21 5737.0 22 11142.5 23 16548.0 24 16548.0 25 16548.0 26 28544.5 27 40541.0 28 40541.0 29 40541.0 30 56402.5 31 72264.0 32 72264.0 33 72264.0 34 74082.0 35 75900.0 36 75900.0 37 75900.0 38 74660.0 39 73420.0 40 73420.0 41 73420.0 42 72296.0 43 71172.0 44 71172.0 45 71172.0 46 71190.0 47 71208.0 48 71208.0 49 71208.0 50 62331.5 51 53455.0 52 53455.0 53 53455.0 54 40263.5 55 27072.0 56 27072.0 57 27072.0 58 21786.5 59 16501.0 60 16501.0 61 16501.0 62 12945.0 63 9389.0 64 9389.0 65 9389.0 66 7108.0 67 4827.0 68 4827.0 69 4827.0 70 3584.0 71 2341.0 72 2341.0 73 2341.0 74 1718.5 75 1096.0 76 1096.0 77 1096.0 78 790.5 79 485.0 80 485.0 81 485.0 82 348.0 83 211.0 84 211.0 85 211.0 86 160.0 87 109.0 88 109.0 89 109.0 90 77.5 91 46.0 92 46.0 93 46.0 94 36.0 95 26.0 96 26.0 97 26.0 98 134.5 99 243.0 100 243.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03415474278571896 2 0.006176921567630024 3 0.0025434382925535394 4 0.003451809111322661 5 0.009810404842706509 6 0.014170584772798292 7 0.021619225486705085 8 0.03560813609574955 9 0.044691844283440765 10 0.055047271617408744 11 0.0546839232899011 12 0.06449432813260761 13 0.06195088984005406 14 0.0659477214426382 15 0.06249591233131554 16 0.06649274393389967 17 0.06267758649506937 18 0.07612147461285236 19 0.07285133966528352 20 0.07902826123291354 21 0.07575812628534472 22 0.07575812628534472 23 0.0817533736892209 24 0.07775654208663677 25 0.07666649710411383 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 550436.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.884974816751814 #Duplication Level Percentage of deduplicated Percentage of total 1 77.89021868077928 36.51880941319629 2 13.258550819375747 12.43253642546112 3 3.8826160223001867 5.46109063285984 4 1.5300070983336889 2.8693737709930605 5 0.8228428019012315 1.928948202264237 6 0.4781453186990234 1.3450698735570887 7 0.34649984206392453 1.1371945458422907 8 0.23462016344152742 0.8800128363566566 9 0.1816492933436632 0.766496028950857 >10 1.1366334356302705 10.215026536625526 >50 0.11522851413493368 3.813027312280432 >100 0.10087344668485018 9.194594576231129 >500 0.01280316823307714 4.324535411702354 >1k 0.008923420283659824 8.147021621070287 >5k 3.879747949417315E-4 0.9662628126088544 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTAAAGTGTGTATTTCTCATTT 5312 0.9650531578603143 No Hit CTGTAGGACGTGGAATATGGCAAGA 4338 0.7881025223640895 No Hit GTCCTACAGTGGACATTTCTAAATT 4298 0.7808355558139366 No Hit CTTTAGGACGTGAAATATGGCGAGG 4166 0.7568545661984317 No Hit GTATCAACGCAGAGTACTTTTTTTT 2736 0.49706051203046314 No Hit GTCCTACAGTGTGCATTTCTCATTT 2468 0.44837183614443826 No Hit ATTTAGAAATGTCCACTGTAGGACG 2311 0.41984899243508783 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2116 0.3844225305030921 No Hit TATCAACGCAGAGTACTTTTTTTTT 2095 0.3806073730642618 No Hit CTGAAGGACCTGGAATATGGCGAGA 2079 0.37770058644420057 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1942 0.35281122600992665 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1719 0.31229788749282383 No Hit CTGTAGGACCTGGAATATGGCGAGA 1646 0.29903567353879473 No Hit GATATACACTGTTCTACAAATCCCG 1454 0.26415423409806044 No Hit GAATATGGCAAGAAAACTGAAAATC 1348 0.24489677274015506 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1334 0.24235333444760152 No Hit TTGTAGAACAGTGTATATCAATGAG 1289 0.23417799707867942 No Hit GGAATATGGCGAGAAAACTGAAAAT 1165 0.21165040077320524 No Hit GTACATGGGGTGGTATCAACGCAAA 1119 0.2032933892405293 No Hit GCCATATTCCACGTCCTACAGTGGA 1065 0.1934829843978228 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1055 0.19166624276028457 No Hit CCATATTCCAGGTCCTTCAGTGTGC 1024 0.18603434368391603 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1011 0.18367257955511632 No Hit GGTATCAACGCAGAGTACTTTTTTT 1010 0.18349090539136248 No Hit GTTCTACAGTGTGGTTTTTATCATT 955 0.17349882638490213 No Hit GAAATATGGCGAGGAAAACTGAAAA 951 0.17277212972988684 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 918 0.16677688232601065 No Hit GTCCACTGTAGGACGTGGAATATGG 897 0.16296172488718036 No Hit GTGTATATCAATGAGTTACAATGAG 891 0.1618716799046574 No Hit CTGTAGGACATGGAATATGGCAAGA 876 0.15914656744835004 No Hit GTGTATATCAATGAGTTACAATGAA 844 0.15333299420822766 No Hit GACCTGGAATATGGCGAGAAAACTG 842 0.15296964588072 No Hit CCATATTTCACGTCCTAAAGTGTGT 836 0.15187960089819708 No Hit ACCTGGAATATGGCGAGAAAACTGA 812 0.14751942096810527 No Hit ACAGTGGACATTTCTAAATTTTCCA 812 0.14751942096810527 No Hit CCACTGTAGGACGTGGAATATGGCA 811 0.14733774680435147 No Hit CCTAAAGTGTGTATTTCTCATTTTC 778 0.14134249940047525 No Hit ATACACACTTTAGGACGTGAAATAT 769 0.13970743192669083 No Hit GTATCAACGCAGAGTACATGGGGTG 755 0.13716399363413728 No Hit CACTTTAGGACGTGAAATATGGCGA 742 0.13480222950533757 No Hit GTTCTACAAATCCCGTTTCCAACGA 737 0.13389385868656847 No Hit GTGTATTTCTCATTTTCCGTGATTT 734 0.133348836195307 No Hit GAAATACACACTTTAGGACGTGAAA 709 0.12880698210146138 No Hit GATATACACTGTTCTACAATGCCGG 697 0.1266268921364155 No Hit AGTGTGTATTTCTCATTTTCCGTGA 672 0.12208503804256989 No Hit CTGTAGAACATATTAGATGAGTGAG 625 0.11354635234614016 No Hit GTACATGGGTGGTATCAACGCAAAA 614 0.11154793654484808 No Hit CTACAGTGGACATTTCTAAATTTTC 605 0.10991286907106367 No Hit GTAGGACGTGGAATATGGCAAGAAA 584 0.10609771163223336 No Hit ATTCCACGTCCTACAGTGGACATTT 568 0.10319092501217217 No Hit TCCTAAAGTGTGTATTTCTCATTTT 568 0.10319092501217217 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGT 40 1.285341E-5 18.995546 1 TTGCGAG 35 0.0021633005 16.290781 18 ATTGCGA 50 8.6745735E-5 15.20473 17 TAAGACC 50 8.708291E-5 15.197819 4 TCCTATA 100 4.0017767E-11 15.193674 2 CCAACGT 70 4.405283E-7 14.933216 19 TATTGCG 45 6.7365874E-4 14.782376 16 CCACCTT 560 0.0 14.589844 13 TAGAAAT 535 0.0 14.381112 4 TATATTG 40 0.0052783918 14.247953 5 TTCCTAT 40 0.005281577 14.246659 1 TAGGGCA 55 1.9589142E-4 13.816197 4 CACCTTT 595 0.0 13.573183 14 ACCTTTT 600 0.0 13.461296 15 CAAGGGC 50 0.0014990204 13.298092 4 TAAGGCT 50 0.0014990204 13.298092 4 AAATGTC 550 0.0 13.29809 7 TCCAACG 230 0.0 13.221504 18 GGACGAG 65 5.447334E-5 13.1519575 6 CTCTTAT 65 5.451984E-5 13.1507635 1 >>END_MODULE