FastQCFastQC Report
Thu 2 Feb 2017
SRR4064074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550436
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA22150.4024082727147207No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA18110.3290119105581757No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA17900.32519675311934537No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC16250.2952205160999644No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA11840.2151022098845279No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA11260.20456510838680608No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG9080.16496014068847242No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8320.15115290424318178No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA7690.13970743192669083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.13770901612539876No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT7290.1324404653765379No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC6100.11082123988983279No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT5640.10246422835715686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC353.215369E-431.428963
AGGCGGA300.00573943229.3363632
GGCGGAT300.00573943229.3363633
AATCGTG300.005741981329.33369834
GGCGATA407.0304587E-427.4978419
TAGGACC8950.025.8103754
GCGATAA450.001397146324.44474820
GTATAGG651.809617E-523.6947541
ATAGGAC1500.023.4669573
CCTACCG752.0606458E-623.4669573
CTACCGT752.0606458E-623.4669574
GTGGCAC500.002578483222.0002733
CGAATTT500.002578483222.00027325
AGATTAT500.002578483222.0002736
GGACCTA1004.6118657E-822.0002736
ACTGTGC1306.730261E-1122.000278
TCCTATA1500.021.9982762
TGTAGGA28650.021.88312
CTACACT1850.021.405674
CTGTAGG28500.021.230261
Produced by FastQC (version 0.11.5)