##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064074_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	550436
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244640612169263	32.0	32.0	32.0	32.0	32.0
2	31.48256836398782	32.0	32.0	32.0	32.0	32.0
3	31.53764470347143	32.0	32.0	32.0	32.0	32.0
4	31.617904715534593	32.0	32.0	32.0	32.0	32.0
5	31.580407168135807	32.0	32.0	32.0	32.0	32.0
6	35.1633595913058	36.0	36.0	36.0	36.0	36.0
7	35.187349664629494	36.0	36.0	36.0	36.0	36.0
8	35.151403614589164	36.0	36.0	36.0	36.0	36.0
9	35.237996788000785	36.0	36.0	36.0	36.0	36.0
10	35.13264030695667	36.0	36.0	36.0	36.0	36.0
11	35.22648954646862	36.0	36.0	36.0	36.0	36.0
12	35.1823554418679	36.0	36.0	36.0	36.0	36.0
13	35.19815019366466	36.0	36.0	36.0	36.0	36.0
14	35.151367279756414	36.0	36.0	36.0	36.0	36.0
15	35.128670726478646	36.0	36.0	36.0	36.0	36.0
16	35.15119650604248	36.0	36.0	36.0	36.0	36.0
17	35.160819786496525	36.0	36.0	36.0	36.0	36.0
18	35.141896605599925	36.0	36.0	36.0	36.0	36.0
19	35.09816945112602	36.0	36.0	36.0	36.0	36.0
20	35.08862610730403	36.0	36.0	36.0	36.0	36.0
21	35.06559527356496	36.0	36.0	36.0	36.0	36.0
22	35.02725112456307	36.0	36.0	36.0	36.0	36.0
23	35.030107042417285	36.0	36.0	36.0	36.0	36.0
24	34.99984921044408	36.0	36.0	36.0	36.0	36.0
25	34.94989426563669	36.0	36.0	36.0	36.0	36.0
26	34.919645517371684	36.0	36.0	36.0	36.0	36.0
27	34.84856913428627	36.0	36.0	36.0	36.0	36.0
28	34.78868024620483	36.0	36.0	36.0	32.0	36.0
29	34.76608361371713	36.0	36.0	36.0	32.0	36.0
30	34.69472926916117	36.0	36.0	36.0	32.0	36.0
31	34.67003066659884	36.0	36.0	36.0	32.0	36.0
32	34.63661352091796	36.0	36.0	36.0	32.0	36.0
33	34.65875960147955	36.0	36.0	36.0	32.0	36.0
34	34.60534376385265	36.0	36.0	36.0	32.0	36.0
35	34.567175838789616	36.0	36.0	36.0	32.0	36.0
36	34.48478297204398	36.0	36.0	36.0	32.0	36.0
37	34.37511717983562	36.0	36.0	36.0	32.0	36.0
38	34.3085408657864	36.0	36.0	36.0	32.0	36.0
39	34.12809481937955	36.0	36.0	36.0	32.0	36.0
40	34.20244315415416	36.0	36.0	36.0	32.0	36.0
41	34.181423453407845	36.0	36.0	36.0	32.0	36.0
42	34.139200561009815	36.0	36.0	36.0	32.0	36.0
43	34.13462782230813	36.0	36.0	36.0	32.0	36.0
44	33.99778175846057	36.0	36.0	36.0	32.0	36.0
45	33.938884811313216	36.0	36.0	36.0	32.0	36.0
46	33.72503070293367	36.0	36.0	36.0	27.0	36.0
47	33.7414867486865	36.0	36.0	36.0	27.0	36.0
48	33.632929168877034	36.0	36.0	36.0	27.0	36.0
49	33.57147243276239	36.0	36.0	36.0	21.0	36.0
50	33.12136924183738	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	0.0
20	5.0
21	10.0
22	67.0
23	159.0
24	456.0
25	1099.0
26	2189.0
27	4308.0
28	7570.0
29	11010.0
30	15402.0
31	21199.0
32	30489.0
33	47265.0
34	92290.0
35	316916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.56742098712719	20.763828956094372	12.785777228667206	25.882972828111235
2	11.038925647761584	21.739754448308062	47.74923060712092	19.47208929680943
3	18.634057187704954	27.615350097105367	30.866790326888104	22.883802388301575
4	10.108168797099026	18.571459715570928	40.6365136001279	30.683857887202144
5	9.671242433271079	42.12333495628919	36.61224919881694	11.593173411622788
6	28.71583384050796	40.15642173916993	19.392480492701228	11.735263927620881
7	25.708394269986467	32.731748526165674	23.164224658679046	18.395632545168823
8	22.055425153878016	43.92808609901969	20.169829008277073	13.846659738825224
9	26.853333866252925	14.22404787555843	21.022042886704323	37.900575371484315
10	15.216984508503337	30.028650275874725	35.79649402014058	18.95787119548136
11	33.24946042773365	20.01994782318017	30.051631797338835	16.67895995174734
12	26.2114033239105	23.813122688196266	34.95901430865714	15.016459679236096
13	31.17970332555161	23.367336742758	26.769373313833604	18.683586617856786
14	18.384005377565018	26.18910498060625	28.411529063377145	27.015360578451585
15	19.375480326376664	40.023836906144	23.10054196258405	17.50014080489529
16	17.156254485137957	27.91640702070567	35.73103888058224	19.196299613574137
17	17.6080416251844	31.969565944087957	31.491944567579154	18.930447863148487
18	17.704724445211514	27.05351222215157	39.01913940792283	16.22262392471409
19	24.289472345558792	24.615395795333153	29.016270738105792	22.078861121002262
20	23.922984268748422	29.266595571123098	30.757421884225693	16.052998275902787
21	21.189566089427288	25.964871483696562	28.03650197298142	24.80906045389473
22	22.9096570718485	32.56709226867429	28.169305786685463	16.35394487279175
23	18.405227855736182	32.410852487846	31.35187378732496	17.832045869092862
24	23.604999727485783	27.464164380575184	32.08963900950167	16.841196882437366
25	20.568106463187537	30.26806558568379	32.095925875459876	17.0679020756688
26	15.864366993279397	32.37154272279827	33.90207432089928	17.862015963023055
27	18.026689922691887	31.51645636315737	31.738842104306908	18.718011609843842
28	16.879266145720727	32.398634470003216	29.476080514671814	21.24601886960424
29	20.160749680698956	28.332312902305688	28.470389029488334	23.03654838750702
30	17.349035538897297	29.6387608532533	31.197777660693554	21.81442594715585
31	22.243358788057176	26.984732616385866	30.667945761997423	20.10396283355954
32	18.68251658300222	31.99833579512112	30.680234660155193	18.63891296172147
33	17.068402606817187	32.328312163996195	33.664424067456025	16.938861161730586
34	19.16420491913477	31.36793224108048	32.821544196997934	16.646318642786806
35	17.628730373864038	32.80712336532138	32.005363235673514	17.558783025141075
36	16.41951038710451	31.251249105186467	33.46838810016097	18.860852407548048
37	17.575411828615344	31.349845842470447	28.60153594314067	22.47320638577354
38	19.384395678669154	34.06354812804105	28.401158459699193	18.150897733590607
39	22.2925133855006	28.547498314889474	29.59998837228316	19.55999992732677
40	23.42234972012434	29.00169505998114	30.987803925344686	16.588151294549828
41	22.534333559225956	26.79607346536848	33.07485887767097	17.594734097734595
42	19.816867119654084	32.98281312452309	31.152211038843063	16.048108716979762
43	16.56519961847663	33.729935958577464	32.1720488713267	17.532815551619205
44	16.245299129771272	33.04362044219973	30.481260105735515	20.229820322293477
45	16.771978271533165	33.188961357484146	30.42712061479207	19.611939756190612
46	18.862889014043567	28.612902639754374	30.35099831041186	22.1732100357902
47	22.65730174792841	29.234061803376676	29.196272684826035	18.91236376386888
48	17.547636696607658	30.81487456759978	28.920125868434056	22.717362867358506
49	21.071487616068136	29.287412954575597	29.081755933077897	20.55934349627837
50	18.060226188854067	34.48825907253486	27.506018076940546	19.94549666167053
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	152.0
1	101.0
2	50.0
3	136.0
4	222.0
5	231.0
6	240.0
7	221.0
8	202.0
9	290.0
10	378.0
11	566.0
12	754.0
13	1350.0
14	1946.0
15	2523.0
16	3100.0
17	3449.0
18	3798.0
19	3779.5
20	3761.0
21	3972.5
22	4184.0
23	4635.0
24	5086.0
25	5810.0
26	6534.0
27	9918.0
28	13302.0
29	18089.0
30	22876.0
31	29192.5
32	35509.0
33	45093.5
34	54678.0
35	64075.0
36	73472.0
37	70957.0
38	68442.0
39	59420.0
40	50398.0
41	43311.0
42	36224.0
43	31434.0
44	26644.0
45	25023.5
46	23403.0
47	22724.5
48	22046.0
49	21131.0
50	20216.0
51	18940.5
52	17665.0
53	16199.5
54	14734.0
55	13147.5
56	11561.0
57	10370.5
58	9180.0
59	7869.0
60	6558.0
61	5454.0
62	4350.0
63	3690.0
64	3030.0
65	2543.5
66	2057.0
67	1715.0
68	1373.0
69	1121.0
70	869.0
71	670.0
72	471.0
73	430.5
74	390.0
75	296.5
76	203.0
77	185.0
78	167.0
79	120.5
80	74.0
81	63.5
82	53.0
83	41.5
84	30.0
85	28.0
86	26.0
87	17.5
88	9.0
89	6.0
90	3.0
91	2.0
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	5.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009265382351445037
2	3.633483275076485E-4
3	5.450224912614727E-4
4	0.0
5	0.0
6	1.8167416375382424E-4
7	1.8167416375382424E-4
8	0.0
9	0.0016350674737844182
10	9.083708187691213E-4
11	0.0
12	0.0
13	1.8167416375382424E-4
14	1.8167416375382424E-4
15	0.005268550748860903
16	0.0012717191462767697
17	0.0
18	1.8167416375382424E-4
19	0.0
20	5.450224912614727E-4
21	0.0
22	0.0
23	0.0
24	0.0010900449825229455
25	0.001998415801292067
26	0.007085292386399146
27	0.007448640713906793
28	0.004905202421353254
29	0.0027251124563073634
30	0.004541854093845606
31	0.00853868569642974
32	0.004178505766337958
33	0.005995247403876199
34	0.0025434382925535394
35	0.003996831602584134
36	0.007630314877660618
37	0.004541854093845606
38	0.009083708187691213
39	0.004905202421353254
40	0.0023617641287997154
41	0.004905202421353254
42	0.002906786620061188
43	0.001998415801292067
44	0.0010900449825229455
45	0.0010900449825229455
46	0.0010900449825229455
47	0.0023617641287997154
48	0.0036334832750764853
49	9.083708187691213E-4
50	0.001998415801292067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	550436.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.13003938402738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.38929934314089	49.14201034900569
2	12.272619645981727	15.004514446081021
3	3.46053791326079	6.34628456782656
4	1.3742032115275684	3.3602038576934863
5	0.6815782624591307	2.0832453013711802
6	0.38283229867271834	1.4041532097204603
7	0.25910632432120445	1.108742586728406
8	0.17182289867734732	0.840283245057919
9	0.14028606387909168	0.7718123348963148
>10	0.7547043633069984	8.646893039290903
>50	0.06112660845107256	2.6009059036867557
>100	0.047112209648171095	5.689036841702101
>500	0.002981785420770198	1.224534603266601
>1k	0.001789071252462119	1.7773797136726148
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2215	0.4024082727147207	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1811	0.3290119105581757	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1790	0.32519675311934537	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1625	0.2952205160999644	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1184	0.2151022098845279	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1126	0.20456510838680608	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	908	0.16496014068847242	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	832	0.15115290424318178	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	769	0.13970743192669083	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	758	0.13770901612539876	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	729	0.1324404653765379	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	610	0.11082123988983279	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	564	0.10246422835715686	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	1.8167416375382424E-4	0.0
18	0.0	0.0	0.0	1.8167416375382424E-4	0.0
19	0.0	0.0	0.0	1.8167416375382424E-4	0.0
20	0.0	0.0	0.0	1.8167416375382424E-4	0.0
21	0.0	0.0	0.0	7.26696655015297E-4	0.0
22	0.0	0.0	0.0	0.0016350674737844182	0.0
23	0.0	0.0	0.0	0.004905202421353254	0.0
24	0.0	0.0	0.0	0.00817533736892209	0.0
25	0.0	0.0	0.0	0.009810404842706509	0.0
26	0.0	0.0	0.0	0.011082123988983278	0.0
27	1.8167416375382424E-4	0.0	0.0	0.01417058477279829	0.0
28	1.8167416375382424E-4	0.0	0.0	0.018167416375382425	0.0
29	1.8167416375382424E-4	0.0	0.0	0.025071034598027744	0.0
30	1.8167416375382424E-4	0.0	0.0	0.04233008015464105	0.0
31	1.8167416375382424E-4	0.0	0.0	0.07521310379408323	0.0
32	1.8167416375382424E-4	0.0	0.0	0.12117666722380077	0.0
33	1.8167416375382424E-4	0.0	0.0	0.16023661243087298	0.0
34	1.8167416375382424E-4	0.0	0.0	0.20438343422305227	0.0
35	1.8167416375382424E-4	0.0	0.0	0.2557972225653845	0.0
36	1.8167416375382424E-4	0.0	0.0	0.31502299994913124	0.0
37	1.8167416375382424E-4	0.0	0.0	0.4074951492998278	0.0
38	1.8167416375382424E-4	0.0	0.0	0.531215254816182	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGTAC	35	3.215369E-4	31.42896	3
AGGCGGA	30	0.005739432	29.33636	32
GGCGGAT	30	0.005739432	29.33636	33
AATCGTG	30	0.0057419813	29.333698	34
GGCGATA	40	7.0304587E-4	27.49784	19
TAGGACC	895	0.0	25.810375	4
GCGATAA	45	0.0013971463	24.444748	20
GTATAGG	65	1.809617E-5	23.694754	1
ATAGGAC	150	0.0	23.466957	3
CCTACCG	75	2.0606458E-6	23.466957	3
CTACCGT	75	2.0606458E-6	23.466957	4
GTGGCAC	50	0.0025784832	22.000273	3
CGAATTT	50	0.0025784832	22.000273	25
AGATTAT	50	0.0025784832	22.000273	6
GGACCTA	100	4.6118657E-8	22.000273	6
ACTGTGC	130	6.730261E-11	22.00027	8
TCCTATA	150	0.0	21.998276	2
TGTAGGA	2865	0.0	21.8831	2
CTACACT	185	0.0	21.40567	4
CTGTAGG	2850	0.0	21.23026	1
>>END_MODULE