FastQCFastQC Report
Thu 2 Feb 2017
SRR4064073_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064073_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275133
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35770.2805197575468598No Hit
TATCAACGCAGAGTACTTTTTTTTT25250.19801855963260304No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21420.16798247712199432No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15110.118497443011827No Hit
GAATAGGACCGCGGTTCTATTTTGT13560.10634184826210287No Hit
GCGCAAGACGGACCAGAGCGAAAGC13140.10304807420088728No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13060.10242068866541765No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG12930.1014011871702795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC902.2973836E-914.7726813
TCGCGTA1006.566552E-1014.2523559
CGTCTTA759.650776E-713.93563615
AACGGAC551.956971E-413.82046615
CGCATCG1650.013.81992313
CGCCAGT1650.013.81992318
ATTGGAC551.9654579E-413.81341653
GCTCGTA1400.013.5736719
CGCGTAA1102.739398E-912.95617710
GCGTTAT1102.748493E-912.9531271
CGTTATT1102.759407E-912.949572
CGGTCCA2050.012.51377210
CTCGTAG1457.2759576E-1212.44984510
GCGTAAC1155.318725E-912.39140611
TCGGGGG1155.349648E-912.3865452
GTGCTAG853.946565E-612.2927721
TTGCACC550.003073149112.0881624
GATATAC2050.012.0474621
ACGGTAT2150.011.9322049
ATCGCCA2000.011.87649616
Produced by FastQC (version 0.11.5)