##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064073_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1275133
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221919595838237	32.0	32.0	32.0	32.0	32.0
2	30.81733513288418	32.0	32.0	32.0	32.0	32.0
3	30.828411624512896	32.0	32.0	32.0	32.0	32.0
4	30.855299800099285	32.0	32.0	32.0	32.0	32.0
5	30.76245615163281	32.0	32.0	32.0	32.0	32.0
6	34.41945506860853	36.0	36.0	36.0	32.0	36.0
7	34.336007302767634	36.0	36.0	36.0	32.0	36.0
8	34.29257340214707	36.0	36.0	36.0	32.0	36.0
9	34.420474570103664	36.0	36.0	36.0	32.0	36.0
10	34.11280862466896	36.0	36.0	36.0	32.0	36.0
11	34.4324521442077	36.0	36.0	36.0	32.0	36.0
12	34.210325511142756	36.0	36.0	36.0	32.0	36.0
13	34.31053466579564	36.0	36.0	36.0	32.0	36.0
14	34.199553301498746	36.0	36.0	36.0	32.0	36.0
15	34.13054638222052	36.0	36.0	36.0	32.0	36.0
16	34.13744448618301	36.0	36.0	36.0	32.0	36.0
17	34.07003975271599	36.0	36.0	36.0	32.0	36.0
18	34.063596503266716	36.0	36.0	36.0	32.0	36.0
19	34.07332568445801	36.0	36.0	36.0	32.0	36.0
20	34.053163866043775	36.0	36.0	36.0	32.0	36.0
21	34.04879726271691	36.0	36.0	36.0	32.0	36.0
22	34.020851158271334	36.0	36.0	36.0	32.0	36.0
23	33.96214747794936	36.0	36.0	36.0	32.0	36.0
24	33.95203559158143	36.0	36.0	36.0	32.0	36.0
25	33.54118903675146	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	10.0
5	17.0
6	45.0
7	18.0
8	71.0
9	82.0
10	125.0
11	30.0
12	99.0
13	72.0
14	120.0
15	233.0
16	351.0
17	500.0
18	599.0
19	842.0
20	1301.0
21	1999.0
22	3110.0
23	4773.0
24	7307.0
25	10650.0
26	14971.0
27	19836.0
28	26912.0
29	36113.0
30	46877.0
31	63694.0
32	88789.0
33	125079.0
34	264413.0
35	556089.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.80238070768509	17.12766433167041	11.240147395821484	25.829807564823014
2	16.524687310794405	19.825687728027056	37.808101214994025	25.84152374618452
3	18.8406047150331	24.050731190098446	28.57205596680451	28.536608128063946
4	12.475305606597823	15.209079851021931	36.04389347550402	36.271721066876225
5	14.461240668788461	36.845403451862005	33.629223652611486	15.064132226738048
6	34.07052888427701	35.56194003452639	17.038715511852466	13.32881556934415
7	30.114571420054943	30.44371127939393	20.94948534637342	18.492231954177708
8	28.04154477265934	33.08519126081748	19.537559829069888	19.33570413745329
9	27.514535225855045	13.85966151166349	18.59857668557619	40.02722657690527
10	15.73831543958053	26.63158539016019	31.89770036111848	25.7323988091408
11	37.409839924670436	20.838983050847457	22.80076898932831	18.9504080351538
12	24.913718282934973	23.484243286976366	29.1551375877531	22.446900842335562
13	29.675406360035815	19.46031589082956	25.46611828759453	25.398159461540093
14	23.43636209537681	19.84615529494488	25.38029557268778	31.33718703699053
15	24.81176456740182	27.69883900230316	22.480901803685843	25.008494626609174
16	25.293014809460917	25.694583414753584	24.45911447726629	24.553287298519212
17	23.68303548657198	25.969993243418216	25.646053991444802	24.700917278564997
18	24.490625627888285	24.829292872212175	26.914588356439623	23.765493143459913
19	25.493524707825056	24.742985721472575	25.611721059748803	24.15176851095357
20	25.78672824341443	24.33226013559652	25.210074140120593	24.670937480868457
21	26.65196440063413	24.206862452714688	24.57721828940966	24.56395485724152
22	25.762167244723127	24.47780663481666	25.23544125761226	24.524584862847963
23	24.14488958797406	24.443151974104545	25.82693246554677	25.585025972374627
24	24.72167435683918	25.03851626630417	25.640806286922054	24.599003089934598
25	24.69282503643717	24.62446364563213	25.660401644754653	25.02230967317604
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	165.0
1	165.0
2	400.5
3	636.0
4	636.0
5	636.0
6	1731.0
7	2826.0
8	2826.0
9	2826.0
10	2895.0
11	2964.0
12	2964.0
13	2964.0
14	3122.0
15	3280.0
16	3280.0
17	3280.0
18	5608.0
19	7936.0
20	7936.0
21	7936.0
22	13238.5
23	18541.0
24	18541.0
25	18541.0
26	28324.0
27	38107.0
28	38107.0
29	38107.0
30	48581.5
31	59056.0
32	59056.0
33	59056.0
34	70658.0
35	82260.0
36	82260.0
37	82260.0
38	93790.0
39	105320.0
40	105320.0
41	105320.0
42	120452.0
43	135584.0
44	135584.0
45	135584.0
46	151382.5
47	167181.0
48	167181.0
49	167181.0
50	169191.5
51	171202.0
52	171202.0
53	171202.0
54	158121.0
55	145040.0
56	145040.0
57	145040.0
58	132881.0
59	120722.0
60	120722.0
61	120722.0
62	106093.5
63	91465.0
64	91465.0
65	91465.0
66	75008.0
67	58551.0
68	58551.0
69	58551.0
70	44463.5
71	30376.0
72	30376.0
73	30376.0
74	23354.0
75	16332.0
76	16332.0
77	16332.0
78	13121.5
79	9911.0
80	9911.0
81	9911.0
82	6783.0
83	3655.0
84	3655.0
85	3655.0
86	2866.5
87	2078.0
88	2078.0
89	2078.0
90	1463.0
91	848.0
92	848.0
93	848.0
94	605.0
95	362.0
96	362.0
97	362.0
98	548.5
99	735.0
100	735.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03685890020884096
2	0.005097507475690771
3	0.0015684638386740833
4	0.0036074668289503915
5	0.008704974304641164
6	0.01301824986099489
7	0.023134841620442728
8	0.03607466828950392
9	0.05042611241337178
10	0.059523202677681464
11	0.05748419968740516
12	0.06422859419370372
13	0.06524809568884188
14	0.0675223682549193
15	0.06258170716309593
16	0.06901240890165967
17	0.06430701738563742
18	0.08007007896431195
19	0.0770115744788975
20	0.08305016025779272
21	0.07473730191282008
22	0.08124642684331752
23	0.08524600963193643
24	0.07810949916596936
25	0.08014850215624565
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1275133.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.25593527971908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.08152173908815	43.69723447036752
2	12.70287839961988	14.038188534310752
3	3.7395355409026276	6.198946014729746
4	1.509592211340532	3.3365571811440184
5	0.7847657823198949	2.168148363880311
6	0.48718825940437693	1.6152025758412343
7	0.3039386204678292	1.175608891910423
8	0.21165829173030726	0.9356301495412606
9	0.17145067119457438	0.852630047207262
>10	0.8420125735700356	8.347095860882282
>50	0.07272306186756503	2.790236954692567
>100	0.08076907459388837	9.173878958783236
>500	0.008974330425274213	3.322980625113872
>1k	0.002991443475091404	2.3476613715954793
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3577	0.2805197575468598	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2525	0.19801855963260304	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2142	0.16798247712199432	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1511	0.118497443011827	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1356	0.10634184826210287	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1314	0.10304807420088728	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1306	0.10242068866541765	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1293	0.1014011871702795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	7.842319193370416E-5	0.0
9	0.0	0.0	0.0	7.842319193370416E-5	0.0
10	0.0	0.0	0.0	7.842319193370416E-5	0.0
11	0.0	0.0	0.0	3.1369276773481666E-4	0.0
12	0.0	0.0	0.0	3.1369276773481666E-4	7.842319193370416E-5
13	0.0	0.0	0.0	3.1369276773481666E-4	7.842319193370416E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATAC	90	2.2973836E-9	14.772681	3
TCGCGTA	100	6.566552E-10	14.252355	9
CGTCTTA	75	9.650776E-7	13.935636	15
AACGGAC	55	1.956971E-4	13.820466	15
CGCATCG	165	0.0	13.819923	13
CGCCAGT	165	0.0	13.819923	18
ATTGGAC	55	1.9654579E-4	13.8134165	3
GCTCGTA	140	0.0	13.573671	9
CGCGTAA	110	2.739398E-9	12.956177	10
GCGTTAT	110	2.748493E-9	12.953127	1
CGTTATT	110	2.759407E-9	12.94957	2
CGGTCCA	205	0.0	12.513772	10
CTCGTAG	145	7.2759576E-12	12.449845	10
GCGTAAC	115	5.318725E-9	12.391406	11
TCGGGGG	115	5.349648E-9	12.386545	2
GTGCTAG	85	3.946565E-6	12.292772	1
TTGCACC	55	0.0030731491	12.088162	4
GATATAC	205	0.0	12.047462	1
ACGGTAT	215	0.0	11.932204	9
ATCGCCA	200	0.0	11.876496	16
>>END_MODULE