FastQCFastQC Report
Thu 2 Feb 2017
SRR4064073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275133
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14250.11175304850552845No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13840.10853769763624659No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13700.10743977294917471No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13080.10257753504928505No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG602.8729838E-421.9999031
GTAGGAC7550.020.3964613
GTCCTAA3000.019.0665821
TAGGACG4700.018.7225864
AAGTCGA600.007412509518.33325240
GTATAGA1701.2732926E-1018.1175671
GTCCTAC5500.017.999921
ACCGTCG3450.017.8535928
TCACGGA3600.017.72353436
TGTAGGA8450.017.703372
CTGTAGG9000.017.5999221
CGGAAAA3750.017.5999239
ATACCGT3900.017.4864166
AATCACG3550.017.35271634
GTATCAA27700.017.3140011
ATTTCGT2800.017.28495842
TCCTACA6500.017.2607842
ACGTATG1154.2718384E-617.2179932
TAGGACC8500.017.0816084
CGAGCCG4400.016.99859215
Produced by FastQC (version 0.11.5)