##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064073_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1275133
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.308523895154465	32.0	32.0	32.0	32.0	32.0
2	31.427065255153774	32.0	32.0	32.0	32.0	32.0
3	31.495786714013363	32.0	32.0	32.0	32.0	32.0
4	31.574234217136567	32.0	32.0	32.0	32.0	32.0
5	31.53489479136686	32.0	32.0	32.0	32.0	32.0
6	35.122641324473605	36.0	36.0	36.0	36.0	36.0
7	35.13608227533912	36.0	36.0	36.0	36.0	36.0
8	35.088573505665686	36.0	36.0	36.0	36.0	36.0
9	35.18440743044059	36.0	36.0	36.0	36.0	36.0
10	35.05192007421971	36.0	36.0	36.0	36.0	36.0
11	35.19166079146254	36.0	36.0	36.0	36.0	36.0
12	35.09173788146021	36.0	36.0	36.0	36.0	36.0
13	35.139351738210834	36.0	36.0	36.0	36.0	36.0
14	35.08099155146953	36.0	36.0	36.0	36.0	36.0
15	35.06076777873367	36.0	36.0	36.0	36.0	36.0
16	35.073171190769905	36.0	36.0	36.0	36.0	36.0
17	35.0297435640047	36.0	36.0	36.0	36.0	36.0
18	35.03210567054574	36.0	36.0	36.0	36.0	36.0
19	35.03099362968412	36.0	36.0	36.0	36.0	36.0
20	35.01558425670107	36.0	36.0	36.0	36.0	36.0
21	35.0131076522998	36.0	36.0	36.0	36.0	36.0
22	35.00337376571699	36.0	36.0	36.0	36.0	36.0
23	34.947848577364084	36.0	36.0	36.0	36.0	36.0
24	34.91810579759131	36.0	36.0	36.0	36.0	36.0
25	34.88979894646284	36.0	36.0	36.0	32.0	36.0
26	34.837815349457664	36.0	36.0	36.0	32.0	36.0
27	34.83240650190999	36.0	36.0	36.0	32.0	36.0
28	34.805275998660534	36.0	36.0	36.0	32.0	36.0
29	34.76927034277993	36.0	36.0	36.0	32.0	36.0
30	34.75045269787544	36.0	36.0	36.0	32.0	36.0
31	34.74120425085069	36.0	36.0	36.0	32.0	36.0
32	34.70348191129867	36.0	36.0	36.0	32.0	36.0
33	34.67906328202627	36.0	36.0	36.0	32.0	36.0
34	34.664243651446554	36.0	36.0	36.0	32.0	36.0
35	34.619933763772096	36.0	36.0	36.0	32.0	36.0
36	34.59837914946912	36.0	36.0	36.0	32.0	36.0
37	34.57980069530002	36.0	36.0	36.0	32.0	36.0
38	34.52999883149444	36.0	36.0	36.0	32.0	36.0
39	34.51801576776697	36.0	36.0	36.0	32.0	36.0
40	34.50604760444597	36.0	36.0	36.0	32.0	36.0
41	34.483979318235825	36.0	36.0	36.0	32.0	36.0
42	34.41122220191933	36.0	36.0	36.0	32.0	36.0
43	34.40201924034591	36.0	36.0	36.0	32.0	36.0
44	34.33486546109308	36.0	36.0	36.0	32.0	36.0
45	34.304406677577944	36.0	36.0	36.0	32.0	36.0
46	34.25939411810376	36.0	36.0	36.0	32.0	36.0
47	34.232814929893586	36.0	36.0	36.0	32.0	36.0
48	34.18406393685992	36.0	36.0	36.0	32.0	36.0
49	34.155730421846194	36.0	36.0	36.0	32.0	36.0
50	33.56484617683018	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	1.0
19	5.0
20	6.0
21	30.0
22	168.0
23	418.0
24	1058.0
25	2343.0
26	4825.0
27	8734.0
28	14612.0
29	22614.0
30	32318.0
31	45238.0
32	65301.0
33	103975.0
34	217793.0
35	755692.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30695748335333	17.94098962377356	11.629844003670502	26.122208889202607
2	15.837976668954024	20.60707773747672	37.31932163513381	26.23562395843545
3	18.216573996376837	24.62047006971838	28.79157419243528	28.3713817414695
4	12.108776104139725	15.855287252388575	36.11787946825939	35.91805717521231
5	14.030614845667081	37.25713317748031	33.491722039975436	15.220529936877172
6	34.155132539531294	35.86837096482003	16.71634791460315	13.260148581045526
7	29.99313012299903	30.631495405965815	21.01531449293093	18.36005997810423
8	28.035036345228303	33.3992610966856	19.335002701678963	19.230699856407135
9	27.58678020387133	13.767039599472364	18.38896478600412	40.257215410652194
10	15.667821849343161	26.995121254969128	31.69921646774468	25.637840427943033
11	37.39319741548528	20.900486459059564	22.64595144192802	19.06036468352713
12	24.802451824595416	23.65590385936515	29.154550274010848	22.38709404202859
13	29.74216726307691	19.463207251972154	25.347709917976925	25.44691556697401
14	23.396377935095195	19.836581240015715	25.383706275992466	31.38333454889662
15	25.006254484995118	27.78693185160205	22.27467188461946	24.932141778783375
16	25.385805010642088	25.84693591064726	24.334379504456816	24.43287957425384
17	23.82990637055115	26.06967273217774	25.48432202758457	24.616098869686535
18	24.583002974601	24.933477318765394	26.857047404615532	23.62647230201807
19	25.675851598109055	24.746222351882004	25.575234563951028	24.002691486057913
20	25.83552396308449	24.37676844991248	25.278560270027796	24.509147316975238
21	26.782069007703512	24.205004497570055	24.590689755500016	24.42223673922642
22	25.858754904398058	24.492307064921174	25.228153028982902	24.420785001697865
23	24.221722750101165	24.351435625084306	25.839055652626726	25.587785972187802
24	24.81960648687309	24.925007587467622	25.676068852641578	24.57931707301771
25	24.82450547442536	24.52876651522887	25.703879897514803	24.942848112830962
26	24.292054588675228	25.620224255585804	26.08686786456826	24.00085329117071
27	25.023704562334174	25.003470394981925	25.286513456897698	24.686311585786203
28	24.332041911095775	24.949336512218842	25.8624556890548	24.85616588763058
29	24.334528001505753	24.746649308687093	25.83855510504976	25.080267584757394
30	24.175624232404715	25.177517438039477	26.14827548886899	24.498582840686826
31	25.152995560128844	24.733792038976123	25.07339002711294	25.039822373782094
32	24.513250045094857	25.17359558933095	24.971805911739565	25.341348453834634
33	24.19278941548307	24.815265044272078	25.686746609990042	25.305198930254807
34	25.052427013911	24.86899128386189	25.754564718541044	24.32401698368606
35	25.447124950297667	24.810856636898855	25.7441233606072	23.997895052196274
36	24.270007301691624	25.466276929399466	25.381417312459853	24.882298456449057
37	25.28217748791452	25.202888594006357	25.072152109194374	24.442781808884746
38	24.575068155366	25.26140602402457	25.42814746029107	24.73537836031836
39	25.318841238943886	24.68241357837824	25.01948888153426	24.97925630114361
40	25.550344676145585	25.092423398765597	25.34416638564516	24.013065539443655
41	24.467571170888558	25.25041173241314	26.05152537055917	24.230491726139125
42	25.60137901719929	25.61949508709438	25.276387702717646	23.50273819298869
43	24.74186621745193	24.80468413020326	25.579281538748695	24.874168113596117
44	24.265637743898612	25.458466654118826	25.096226237530583	25.179669364451975
45	24.48843869891053	25.61006214336578	25.113323023160245	24.788176134563443
46	24.373943240029988	25.100539241673754	25.252681307857117	25.27283621043914
47	24.913085589029922	24.992530026938958	25.550601715152872	24.54378266887825
48	25.320521869520075	25.539326864861643	24.588505444244724	24.55164582137356
49	24.674287856365908	25.58274496149017	24.804470763350217	24.938496418793708
50	24.627545150617077	26.054782252037256	24.572099432132326	24.74557316521334
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	36.5
2	29.0
3	114.0
4	199.0
5	241.0
6	283.0
7	283.0
8	283.0
9	368.5
10	454.0
11	676.0
12	898.0
13	1606.0
14	2314.0
15	3079.5
16	3845.0
17	4113.0
18	4381.0
19	4421.0
20	4461.0
21	4825.0
22	5189.0
23	5781.5
24	6374.0
25	7162.5
26	7951.0
27	9975.5
28	12000.0
29	14300.5
30	16601.0
31	19156.5
32	21712.0
33	25969.0
34	30226.0
35	34860.5
36	39495.0
37	44956.0
38	50417.0
39	53689.0
40	56961.0
41	61190.5
42	65420.0
43	66962.0
44	68504.0
45	75608.5
46	82713.0
47	87992.0
48	93271.0
49	96231.0
50	99191.0
51	95178.0
52	91165.0
53	90451.0
54	89737.0
55	89897.5
56	90058.0
57	87466.5
58	84875.0
59	76758.5
60	68642.0
61	60254.0
62	51866.0
63	44954.5
64	38043.0
65	32557.0
66	27071.0
67	23218.0
68	19365.0
69	17582.0
70	15799.0
71	12260.0
72	8721.0
73	7518.5
74	6316.0
75	4934.5
76	3553.0
77	3106.0
78	2659.0
79	2184.0
80	1709.0
81	1390.0
82	1071.0
83	854.0
84	637.0
85	472.5
86	308.0
87	213.0
88	118.0
89	93.0
90	68.0
91	61.5
92	55.0
93	41.0
94	27.0
95	24.5
96	22.0
97	19.5
98	17.0
99	16.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00807758876917153
2	6.273855354696334E-4
3	2.352695758011125E-4
4	0.0
5	0.0
6	3.136927677348167E-4
7	7.842319193370418E-5
8	0.0
9	0.0011763478790055627
10	7.842319193370416E-4
11	0.0
12	7.842319193370418E-5
13	3.136927677348167E-4
14	3.136927677348167E-4
15	0.0037643132128178
16	5.489623435359293E-4
17	0.0
18	3.136927677348167E-4
19	7.842319193370418E-5
20	3.921159596685208E-4
21	0.0
22	1.5684638386740836E-4
23	7.058087274033375E-4
24	3.136927677348167E-4
25	0.0017253102225414916
26	0.005489623435359292
27	0.004862237899889659
28	0.004156429172486321
29	0.0018037334144751958
30	0.003685890020884096
31	0.0075286264256356
32	0.0033721972531492797
33	0.004940661091823362
34	0.0021174261822100126
35	0.003293774061215575
36	0.006744394506298559
37	0.0038427364047515043
38	0.008234435153038937
39	0.003685890020884096
40	0.0018037334144751958
41	0.0025879653338122376
42	0.0017253102225414916
43	0.0014900406467403791
44	0.0010195014951381543
45	0.001333194262872971
46	7.058087274033375E-4
47	0.002195849374143717
48	0.0019605797983426043
49	3.136927677348167E-4
50	9.4107830320445E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1275133.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.90797018245573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9804096830445	47.11483588789378
2	12.385005710799675	14.591510942426622
3	3.527603252871285	6.234118417070264
4	1.4307662233431295	3.3713413609104745
5	0.7222591476774721	2.1273410167695204
6	0.4544926892962829	1.606394507352573
7	0.2830261085888415	1.1670745495925565
8	0.19699300403221345	0.9283566406145608
9	0.14688626442735828	0.7787494516589215
>10	0.7372595577165553	7.720756211071285
>50	0.06246007814883765	2.562801075025038
>100	0.06438744444402729	7.900673493016559
>500	0.006707012388796465	2.6846806210965033
>1k	0.0017438232210870807	1.2113658255013804
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1425	0.11175304850552845	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1384	0.10853769763624659	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1370	0.10743977294917471	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1308	0.10257753504928505	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	7.842319193370416E-5	0.0
9	0.0	0.0	0.0	7.842319193370416E-5	0.0
10	0.0	0.0	0.0	7.842319193370416E-5	0.0
11	0.0	0.0	0.0	3.1369276773481666E-4	0.0
12	0.0	0.0	0.0	3.1369276773481666E-4	0.0
13	0.0	0.0	0.0	3.1369276773481666E-4	0.0
14	0.0	0.0	0.0	3.1369276773481666E-4	0.0
15	0.0	0.0	0.0	3.1369276773481666E-4	7.842319193370416E-5
16	0.0	0.0	0.0	3.1369276773481666E-4	7.842319193370416E-5
17	0.0	0.0	0.0	3.1369276773481666E-4	7.842319193370416E-5
18	0.0	0.0	0.0	3.1369276773481666E-4	7.842319193370416E-5
19	0.0	0.0	0.0	3.1369276773481666E-4	7.842319193370416E-5
20	0.0	0.0	0.0	5.489623435359291E-4	7.842319193370416E-5
21	0.0	0.0	0.0	5.489623435359291E-4	7.842319193370416E-5
22	0.0	0.0	0.0	7.842319193370417E-4	7.842319193370416E-5
23	0.0	0.0	0.0	9.410783032044501E-4	7.842319193370416E-5
24	0.0	0.0	0.0	0.0015684638386740835	7.842319193370416E-5
25	0.0	0.0	0.0	0.002274272566077421	7.842319193370416E-5
26	0.0	0.0	0.0	0.0030585044854144626	7.842319193370416E-5
27	0.0	0.0	0.0	0.0040780059805526165	7.842319193370416E-5
28	0.0	0.0	0.0	0.007214933657900784	7.842319193370416E-5
29	0.0	0.0	0.0	0.011449786022320808	7.842319193370416E-5
30	0.0	0.0	0.0	0.022350609701105688	7.842319193370416E-5
31	0.0	0.0	0.0	0.04227010045226655	7.842319193370416E-5
32	0.0	0.0	0.0	0.06493440292110705	7.842319193370416E-5
33	0.0	0.0	0.0	0.086422357510942	7.842319193370416E-5
34	0.0	0.0	0.0	0.11339993553613623	7.842319193370416E-5
35	0.0	0.0	0.0	0.14414182677414827	7.842319193370416E-5
36	0.0	0.0	0.0	0.18609823445867998	7.842319193370416E-5
37	0.0	0.0	0.0	0.24530774436862665	7.842319193370416E-5
38	0.0	0.0	0.0	0.3292205597376901	7.842319193370416E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTACG	60	2.8729838E-4	21.999903	1
GTAGGAC	755	0.0	20.396461	3
GTCCTAA	300	0.0	19.066582	1
TAGGACG	470	0.0	18.722586	4
AAGTCGA	60	0.0074125095	18.333252	40
GTATAGA	170	1.2732926E-10	18.117567	1
GTCCTAC	550	0.0	17.99992	1
ACCGTCG	345	0.0	17.853592	8
TCACGGA	360	0.0	17.723534	36
TGTAGGA	845	0.0	17.70337	2
CTGTAGG	900	0.0	17.599922	1
CGGAAAA	375	0.0	17.59992	39
ATACCGT	390	0.0	17.486416	6
AATCACG	355	0.0	17.352716	34
GTATCAA	2770	0.0	17.314001	1
ATTTCGT	280	0.0	17.284958	42
TCCTACA	650	0.0	17.260784	2
ACGTATG	115	4.2718384E-6	17.21799	32
TAGGACC	850	0.0	17.081608	4
CGAGCCG	440	0.0	16.998592	15
>>END_MODULE