FastQCFastQC Report
Thu 2 Feb 2017
SRR4064072_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064072_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458804
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66260.2694806092718249No Hit
TATCAACGCAGAGTACTTTTTTTTT45180.18374787091610392No Hit
GCGCAAGACGGACCAGAGCGAAAGC28700.11672341512377563No Hit
GTCTTGCGCCGGTCCAAGAATTTCA28610.1163573835084049No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG27980.11379516220080983No Hit
GAATAGGACCGCGGTTCTATTTTGT26660.10842669850870586No Hit
GTATCTGATCGTCTTCGAACCTCCG26190.10651520007288096No Hit
GGGTAGGCACACGCTGAGCCAGTCA26110.10618983863699587No Hit
GAACTACGACGGTATCTGATCGTCT25920.10541710522676878No Hit
GATTAAGAGGGACGGCCGGGGGCAT25320.10297689445763061No Hit
GAATAACGCCGCCGCATCGCCAGTC25100.10208215050894662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG3350.012.1908181
GCGTTAT2500.012.1567971
CGAACGA1950.011.69398616
ACTCTAA3900.011.69255810
CGAACCT4500.011.61277816
GTATCAA41200.011.5952451
GTAATAC1001.93702E-611.3969973
TCGTCGC1701.0913936E-1111.17807413
ACTGCGT951.3612658E-510.9997898
CGTTATT2850.010.9959842
GGTTCTA5400.010.90897413
CGGTTCT5400.010.908751512
CGCATCG5150.010.88505713
CGCCAGT5250.010.85892218
CGCGTAA2450.010.85737510
GTCTAGC1158.8525303E-710.7363021
CGGTCCA6250.010.64022810
TAAGGCC1701.4006218E-1010.615934
AAGACGG6200.010.570875
CGTCGTA4050.010.55578110
Produced by FastQC (version 0.11.5)