##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064072_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2458804
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.252779806767844	32.0	32.0	32.0	32.0	32.0
2	30.848550352122412	32.0	32.0	32.0	32.0	32.0
3	30.861750265576273	32.0	32.0	32.0	32.0	32.0
4	30.89094332041106	32.0	32.0	32.0	32.0	32.0
5	30.800731981890383	32.0	32.0	32.0	32.0	32.0
6	34.45479102848377	36.0	36.0	36.0	32.0	36.0
7	34.3883156201145	36.0	36.0	36.0	32.0	36.0
8	34.34180235594216	36.0	36.0	36.0	32.0	36.0
9	34.49127705990392	36.0	36.0	36.0	32.0	36.0
10	34.18042430384854	36.0	36.0	36.0	32.0	36.0
11	34.46496914760184	36.0	36.0	36.0	32.0	36.0
12	34.267778562260354	36.0	36.0	36.0	32.0	36.0
13	34.36135169781731	36.0	36.0	36.0	32.0	36.0
14	34.247872542911104	36.0	36.0	36.0	32.0	36.0
15	34.182973510698694	36.0	36.0	36.0	32.0	36.0
16	34.181165314518765	36.0	36.0	36.0	32.0	36.0
17	34.09898959006086	36.0	36.0	36.0	32.0	36.0
18	34.09501245320896	36.0	36.0	36.0	32.0	36.0
19	34.11283168564879	36.0	36.0	36.0	32.0	36.0
20	34.09165187627806	36.0	36.0	36.0	32.0	36.0
21	34.0781200941596	36.0	36.0	36.0	32.0	36.0
22	34.06130338164408	36.0	36.0	36.0	32.0	36.0
23	34.00407352517728	36.0	36.0	36.0	32.0	36.0
24	33.988552564580175	36.0	36.0	36.0	32.0	36.0
25	33.59346820649389	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	20.0
5	36.0
6	95.0
7	39.0
8	158.0
9	173.0
10	218.0
11	62.0
12	155.0
13	137.0
14	233.0
15	407.0
16	671.0
17	869.0
18	1178.0
19	1581.0
20	2499.0
21	3818.0
22	6001.0
23	9242.0
24	13664.0
25	20112.0
26	27968.0
27	36885.0
28	49495.0
29	66691.0
30	87896.0
31	118888.0
32	167640.0
33	237431.0
34	510010.0
35	1094523.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.13017342216346	16.642994456593602	11.110410415501196	26.11642170574175
2	17.425319015495443	19.300000244035566	36.35666286242332	26.918017878045664
3	19.30160703021321	22.977119758382177	27.85392956365386	29.867343647750754
4	12.994307542384325	14.91565433978717	34.59457304941001	37.49546506841849
5	15.309002817066395	35.828602294164206	32.765906907466324	16.096487981303074
6	35.39197326482499	34.45196307968651	16.34202215674375	13.814041498744755
7	31.35376550851074	29.84512947641144	19.96097218871822	18.840132826359596
8	29.019375380658307	31.81573147625648	18.758963396995746	20.405929746089466
9	27.58568633706168	14.089050638680192	18.072256800218753	40.25300622403938
10	16.394708806095633	25.94534147538462	30.508031770603157	27.151917947916594
11	37.81953045331024	20.875392787761545	21.8288263286013	19.476250430326917
12	25.544343721067463	23.146028548464223	27.75024672140321	23.559381009065103
13	29.64149059040996	19.007526532512554	25.010520215499593	26.340462661577895
14	24.258552997593526	19.124210814200847	24.41161831265127	32.20561787555436
15	25.58788100760998	26.566678875188217	21.842428681886624	26.00301143531518
16	26.442426849522683	25.405050788287788	22.809616463878434	25.342905898311102
17	24.67404300621921	25.32616047526764	24.415784303112535	25.58401221540062
18	25.467826831623043	24.430947717685598	25.378445613400995	24.722779837290364
19	25.959184470871893	24.420329700519787	24.987657496651345	24.63282833195697
20	26.433387399744795	23.5652897708656	24.33228660218043	25.669036227209176
21	27.48532822885806	23.47316507721631	24.084497354217397	24.95700933970823
22	26.725353732162795	23.39941869197146	24.460727111250765	25.41450046461498
23	25.08192657604101	23.46997463746142	25.099510618197122	26.348588168300452
24	25.278859886872734	24.371489194217542	24.770409938788195	25.579240980121526
25	25.376341897735944	23.92400915797507	24.72938183668278	25.970267107606208
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	193.0
1	193.0
2	465.0
3	737.0
4	737.0
5	737.0
6	2113.5
7	3490.0
8	3490.0
9	3490.0
10	3638.0
11	3786.0
12	3786.0
13	3786.0
14	4241.5
15	4697.0
16	4697.0
17	4697.0
18	8078.0
19	11459.0
20	11459.0
21	11459.0
22	19252.0
23	27045.0
24	27045.0
25	27045.0
26	41567.0
27	56089.0
28	56089.0
29	56089.0
30	70501.0
31	84913.0
32	84913.0
33	84913.0
34	108845.0
35	132777.0
36	132777.0
37	132777.0
38	157302.5
39	181828.0
40	181828.0
41	181828.0
42	215229.5
43	248631.0
44	248631.0
45	248631.0
46	282310.5
47	315990.0
48	315990.0
49	315990.0
50	330251.5
51	344513.0
52	344513.0
53	344513.0
54	323297.0
55	302081.0
56	302081.0
57	302081.0
58	281657.5
59	261234.0
60	261234.0
61	261234.0
62	231472.0
63	201710.0
64	201710.0
65	201710.0
66	167058.0
67	132406.0
68	132406.0
69	132406.0
70	100457.0
71	68508.0
72	68508.0
73	68508.0
74	52807.0
75	37106.0
76	37106.0
77	37106.0
78	29899.0
79	22692.0
80	22692.0
81	22692.0
82	15587.0
83	8482.0
84	8482.0
85	8482.0
86	6473.0
87	4464.0
88	4464.0
89	4464.0
90	3181.0
91	1898.0
92	1898.0
93	1898.0
94	1304.5
95	711.0
96	711.0
97	711.0
98	1037.5
99	1364.0
100	1364.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03778259674215594
2	0.005937846204902872
3	0.001830158076853625
4	0.004555060102391244
5	0.008866099127868671
6	0.013014457435403554
7	0.02265328997349931
8	0.03623712992170177
9	0.05026834184424623
10	0.05848371810034472
11	0.055718145895321466
12	0.06421821340781941
13	0.06629239256158685
14	0.06844791207432557
15	0.061167949946396696
16	0.06682110489490012
17	0.06417754322833377
18	0.07776138317653623
19	0.07511782150996989
20	0.07926617981750476
21	0.0762565865355677
22	0.07853411658676332
23	0.08260113453532693
24	0.07829009550984951
25	0.07845277622779205
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2458804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.01403284150003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.85936558315838	33.98567273595807
2	14.250032903437523	13.114029640224606
3	4.85511627548375	6.702104392482319
4	2.237899441956291	4.118991136726055
5	1.2007221170081068	2.7625033462763238
6	0.7661987460404808	2.1153536558053694
7	0.5103698470972375	1.6438922433950547
8	0.3468930571283433	1.2769558818553548
9	0.27034605762986186	1.1195741136915471
>10	1.5037636219445223	12.320122280039469
>50	0.1023930630445001	3.2429198447319973
>100	0.0766561800781157	7.749211094979646
>500	0.01322904733754361	4.2678344635735765
>1k	0.006925273102875057	5.310137803120849
>5k	8.878555260096225E-5	0.27069736713991294
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6626	0.2694806092718249	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4518	0.18374787091610392	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2870	0.11672341512377563	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2861	0.1163573835084049	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2798	0.11379516220080983	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2666	0.10842669850870586	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2619	0.10651520007288096	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2611	0.10618983863699587	No Hit
GAACTACGACGGTATCTGATCGTCT	2592	0.10541710522676878	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2532	0.10297689445763061	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2510	0.10208215050894662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0670179485636106E-5
2	0.0	0.0	0.0	0.0	4.0670179485636106E-5
3	0.0	0.0	0.0	0.0	4.0670179485636106E-5
4	0.0	0.0	0.0	4.0670179485636106E-5	4.0670179485636106E-5
5	0.0	0.0	0.0	4.0670179485636106E-5	8.134035897127221E-5
6	0.0	0.0	0.0	4.0670179485636106E-5	1.6268071794254443E-4
7	0.0	0.0	0.0	8.134035897127221E-5	1.6268071794254443E-4
8	0.0	0.0	0.0	8.134035897127221E-5	2.0335089742818052E-4
9	0.0	0.0	0.0	1.6268071794254443E-4	2.0335089742818052E-4
10	0.0	0.0	0.0	1.6268071794254443E-4	2.0335089742818052E-4
11	0.0	0.0	0.0	1.6268071794254443E-4	2.0335089742818052E-4
12	0.0	0.0	0.0	1.6268071794254443E-4	2.846912563994527E-4
13	0.0	0.0	0.0	1.6268071794254443E-4	3.2536143588508885E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	335	0.0	12.190818	1
GCGTTAT	250	0.0	12.156797	1
CGAACGA	195	0.0	11.693986	16
ACTCTAA	390	0.0	11.692558	10
CGAACCT	450	0.0	11.612778	16
GTATCAA	4120	0.0	11.595245	1
GTAATAC	100	1.93702E-6	11.396997	3
TCGTCGC	170	1.0913936E-11	11.178074	13
ACTGCGT	95	1.3612658E-5	10.999789	8
CGTTATT	285	0.0	10.995984	2
GGTTCTA	540	0.0	10.908974	13
CGGTTCT	540	0.0	10.9087515	12
CGCATCG	515	0.0	10.885057	13
CGCCAGT	525	0.0	10.858922	18
CGCGTAA	245	0.0	10.857375	10
GTCTAGC	115	8.8525303E-7	10.736302	1
CGGTCCA	625	0.0	10.640228	10
TAAGGCC	170	1.4006218E-10	10.61593	4
AAGACGG	620	0.0	10.57087	5
CGTCGTA	405	0.0	10.555781	10
>>END_MODULE