FastQCFastQC Report
Thu 2 Feb 2017
SRR4064072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458804
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT28010.11391717273926674No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA26680.10850803886767713No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA26610.10822334761127768No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26510.10781664581642131No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT26160.10639318953442406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49700.022.7528951
CGACTAT550.00448553219.9995214
AACGCAG62600.017.81716
GTCCTAT8300.017.2292021
TAACGGC2300.017.21907836
TTAACGG2201.8189894E-1217.00166535
ATACCGT6800.016.8227836
TACCGTC6700.016.7455257
CTATTCC7900.016.7081184
CCGTCGT6900.016.2604799
GTATTAG5450.016.1470581
GGTATCA24750.016.0891571
ACCGTCG7000.016.027868
TCAACGC69800.016.0107524
TATCAAC71000.015.8947472
ATCAACG70700.015.837733
CAACGCA71400.015.6827775
CGTCGTA7050.015.60246210
CCTATTC8750.015.3361513
ACGCAGA75150.014.8416577
Produced by FastQC (version 0.11.5)