##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064072_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2458804
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32521665004612	32.0	32.0	32.0	32.0	32.0
2	31.430213632318804	32.0	32.0	32.0	32.0	32.0
3	31.490983421208036	32.0	32.0	32.0	32.0	32.0
4	31.566429451066455	32.0	32.0	32.0	32.0	32.0
5	31.518798163660055	32.0	32.0	32.0	32.0	32.0
6	35.10635333275853	36.0	36.0	36.0	36.0	36.0
7	35.120359329169794	36.0	36.0	36.0	36.0	36.0
8	35.0708852759309	36.0	36.0	36.0	36.0	36.0
9	35.1636332135461	36.0	36.0	36.0	36.0	36.0
10	35.0321176474416	36.0	36.0	36.0	36.0	36.0
11	35.16810449307875	36.0	36.0	36.0	36.0	36.0
12	35.07198337077701	36.0	36.0	36.0	36.0	36.0
13	35.11861864548781	36.0	36.0	36.0	36.0	36.0
14	35.048002606145104	36.0	36.0	36.0	36.0	36.0
15	35.03372127261872	36.0	36.0	36.0	36.0	36.0
16	35.03755037001729	36.0	36.0	36.0	36.0	36.0
17	34.995844321060154	36.0	36.0	36.0	36.0	36.0
18	34.99322760171205	36.0	36.0	36.0	36.0	36.0
19	34.99755409540573	36.0	36.0	36.0	36.0	36.0
20	34.984611217486226	36.0	36.0	36.0	36.0	36.0
21	34.979444884586165	36.0	36.0	36.0	36.0	36.0
22	34.97295677085282	36.0	36.0	36.0	36.0	36.0
23	34.911458579049004	36.0	36.0	36.0	32.0	36.0
24	34.88271167608317	36.0	36.0	36.0	32.0	36.0
25	34.859428811731235	36.0	36.0	36.0	32.0	36.0
26	34.79131805544484	36.0	36.0	36.0	32.0	36.0
27	34.79253328040787	36.0	36.0	36.0	32.0	36.0
28	34.7670786284714	36.0	36.0	36.0	32.0	36.0
29	34.7424015903667	36.0	36.0	36.0	32.0	36.0
30	34.72411221065201	36.0	36.0	36.0	32.0	36.0
31	34.71646214989076	36.0	36.0	36.0	32.0	36.0
32	34.68265140287717	36.0	36.0	36.0	32.0	36.0
33	34.64545730363217	36.0	36.0	36.0	32.0	36.0
34	34.63872801573448	36.0	36.0	36.0	32.0	36.0
35	34.597767451167314	36.0	36.0	36.0	32.0	36.0
36	34.57766784176372	36.0	36.0	36.0	32.0	36.0
37	34.57277887948775	36.0	36.0	36.0	32.0	36.0
38	34.53068402361473	36.0	36.0	36.0	32.0	36.0
39	34.52761464516895	36.0	36.0	36.0	32.0	36.0
40	34.501874081870696	36.0	36.0	36.0	32.0	36.0
41	34.4826444076063	36.0	36.0	36.0	32.0	36.0
42	34.415596769811664	36.0	36.0	36.0	32.0	36.0
43	34.40022547547507	36.0	36.0	36.0	32.0	36.0
44	34.33513407331369	36.0	36.0	36.0	32.0	36.0
45	34.30201431264956	36.0	36.0	36.0	32.0	36.0
46	34.280398112252946	36.0	36.0	36.0	32.0	36.0
47	34.245710922871446	36.0	36.0	36.0	32.0	36.0
48	34.195928996373844	36.0	36.0	36.0	32.0	36.0
49	34.1756195288441	36.0	36.0	36.0	32.0	36.0
50	33.58578520288726	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	9.0
20	25.0
21	81.0
22	326.0
23	887.0
24	2236.0
25	4913.0
26	9881.0
27	17772.0
28	29202.0
29	44239.0
30	63176.0
31	88409.0
32	125943.0
33	201777.0
34	415553.0
35	1454370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.137999478562705	17.540182600670548	11.661119446967346	26.660698473799403
2	16.574270383883473	19.952879319243223	35.93112555631971	27.541724740553597
3	18.431767131271318	23.528780177916563	28.272733816958155	29.76671887385396
4	12.628700782982296	15.55252065638416	34.66372268794097	37.15505587269258
5	14.792272991259164	36.27934556800786	32.614026982223876	16.314354458509094
6	35.407611602249716	34.808619980730434	16.120553164370087	13.663215252649769
7	31.252183482925215	29.96403531640015	19.980714177357175	18.803067023317464
8	29.00971366566835	32.01690740701577	18.663016653625096	20.310362273690785
9	27.597483939084842	13.995690517674733	18.045123484807814	40.36170205843261
10	16.34000086221552	26.15750399994794	30.55207822409632	26.950416913740217
11	38.042820022588224	20.790848229809384	21.74968877132491	19.416642976277483
12	25.40011387668781	23.19269562388157	27.771636570684887	23.63555392874573
13	29.684911082569617	19.001438914461456	25.04207340334586	26.27157659962307
14	24.292305783930747	19.04908705280432	24.452302855667103	32.206304307597826
15	25.711040079522117	26.598220920353015	21.7577511899644	25.932987810160462
16	26.495766818543597	25.345078410550887	22.85881383071097	25.300340940194545
17	24.74052425488164	25.335569650935984	24.368758144203444	25.555147949978934
18	25.604086554592797	24.32984449699955	25.395041066864053	24.6710278815436
19	26.063199832113497	24.28294406548875	25.002033508974282	24.65182259342347
20	26.513946499679314	23.41974036812431	24.416003162532448	25.650309969663926
21	27.567101729133352	23.47417687623739	24.07629888352223	24.882422511107023
22	26.7697141818309	23.381843426938577	24.526100322880946	25.322342068349574
23	25.141401599250358	23.29755809493907	25.23189374229546	26.329146563515117
24	25.393057908783074	24.256648980518122	24.80744656535429	25.542846545344517
25	25.483678371473122	23.670001407212247	24.960854283578485	25.885465937736146
26	25.305917426901537	24.66992316984386	25.135052691089083	24.88910671216552
27	26.015063465361678	24.008404564430112	24.39499663231739	25.58153533789082
28	25.22200666203009	23.889193926896624	25.329258025549983	25.559541385523303
29	25.066385331414203	24.1988310325039	25.24078282022051	25.494000815861384
30	24.940070394408796	24.49565179902697	25.404092701969077	25.160185104595158
31	25.63244468269779	24.232073582322542	24.29292037773821	25.84256135724146
32	25.579334346594802	24.145014879651136	24.196667625157247	26.078983148596812
33	25.063621355625994	23.855700217229767	24.775866143189436	26.30481228395481
34	25.84282229912723	23.881324243014593	24.886923468306644	25.388929989551528
35	26.256276604138552	23.837592842495365	24.994814404486213	24.91131614887987
36	25.11742397743117	24.454244915423825	24.53062947711961	25.897701630025395
37	26.1415589650066	24.311217092138556	24.46023766014932	25.08698628270552
38	25.2583267544154	23.98919552544099	24.905078517911132	25.84739920223248
39	25.94930479352046	23.99737421574218	24.2902532808068	25.76306770993056
40	25.9699366708924	24.211111124668175	24.796247513465214	25.02270469097421
41	25.021586391320703	24.71565547184008	25.11549685304375	25.147261283795462
42	26.249846975773572	24.58278783501121	24.590962684051032	24.576402505164186
43	25.674809498090102	23.63651226955939	24.753087725234753	25.93559050711576
44	25.07313589387253	24.38681963690108	24.495410120226342	26.044634349000052
45	25.2547502197227	24.56274064261925	24.542771397696665	25.639737739961387
46	25.17269722460258	24.331793411618673	24.68009580349303	25.815413560285716
47	25.33934774465676	24.129836124779917	24.9781088930563	25.552707237507022
48	26.14486849843457	24.74647728076904	23.989020455076027	25.119633765720362
49	25.28531252097064	24.824883733698826	24.227883983821343	25.661919761509193
50	25.293368109514873	24.95096127292863	24.2819278411857	25.473742776370795
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	28.0
2	36.0
3	114.0
4	192.0
5	257.5
6	323.0
7	340.5
8	358.0
9	445.0
10	532.0
11	875.5
12	1219.0
13	2326.5
14	3434.0
15	4664.0
16	5894.0
17	6464.0
18	7034.0
19	7208.0
20	7382.0
21	7637.0
22	7892.0
23	8548.5
24	9205.0
25	9874.0
26	10543.0
27	12751.0
28	14959.0
29	17696.0
30	20433.0
31	23983.0
32	27533.0
33	32455.5
34	37378.0
35	44126.0
36	50874.0
37	62972.5
38	75071.0
39	84945.0
40	94819.0
41	105568.0
42	116317.0
43	122051.0
44	127785.0
45	142148.0
46	156511.0
47	168146.5
48	179782.0
49	188292.5
50	196803.0
51	193476.5
52	190150.0
53	189564.0
54	188978.0
55	190570.5
56	192163.0
57	187220.5
58	182278.0
59	167152.0
60	152026.0
61	134863.5
62	117701.0
63	102443.5
64	87186.0
65	74137.5
66	61089.0
67	52684.5
68	44280.0
69	39747.5
70	35215.0
71	27008.5
72	18802.0
73	16346.0
74	13890.0
75	10810.5
76	7731.0
77	6711.0
78	5691.0
79	4779.0
80	3867.0
81	3148.0
82	2429.0
83	1994.0
84	1559.0
85	1131.5
86	704.0
87	516.5
88	329.0
89	233.5
90	138.0
91	119.0
92	100.0
93	81.5
94	63.0
95	56.5
96	50.0
97	39.5
98	29.0
99	28.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008744088589411763
2	4.473719743419972E-4
3	4.473719743419972E-4
4	0.0
5	0.0
6	2.0335089742818052E-4
7	1.2201053845690831E-4
8	0.0
9	0.0016268071794254442
10	8.947439486839944E-4
11	4.0670179485636106E-5
12	1.6268071794254443E-4
13	2.4402107691381662E-4
14	3.66031615370725E-4
15	0.0047584109998194245
16	6.100526922845416E-4
17	0.0
18	3.66031615370725E-4
19	0.0
20	6.100526922845416E-4
21	0.0
22	1.2201053845690831E-4
23	0.0010980948461121749
24	2.4402107691381662E-4
25	0.0017081475383967165
26	0.005449804051075239
27	0.005693825127989055
28	0.0038229968716497935
29	0.0021555195127387137
30	0.0034976354357647054
31	0.006913930512558138
32	0.0031316038203939803
33	0.0047584109998194245
34	0.0028875827434801634
35	0.0028469125639945273
36	0.007808674461242132
37	0.003375624897307797
38	0.007849344640727769
39	0.0044737197434199715
40	0.0024808809486238023
41	0.003212944179365252
42	0.0017488177178823527
43	0.0014641264614829
44	0.0010167544871409026
45	9.354141281696305E-4
46	8.540737691983582E-4
47	0.0017488177178823527
48	0.0020335089742818052
49	3.66031615370725E-4
50	0.0013421159230259916
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2458804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.121792716694515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.84797299808528	36.76666614876692
2	14.035736480477478	13.78921076040324
3	4.658304575880768	6.864728153629339
4	2.0766179719919196	4.080287902877984
5	1.1456104244678835	2.8137218902397896
6	0.689470579136529	2.032081852356234
7	0.46129006899552505	1.5861576606027525
8	0.32894961766359393	1.2926875946485579
9	0.23858080459858658	1.054756514670657
>10	1.348695190277888	11.777758300055389
>50	0.08425450704434757	2.8596474352085473
>100	0.06773940049794576	7.0727693789096495
>500	0.011602268371245172	3.944080624785144
>1k	0.005175112510914608	4.065445782845874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2801	0.11391717273926674	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2668	0.10850803886767713	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2661	0.10822334761127768	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2651	0.10781664581642131	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2616	0.10639318953442406	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.134035897127221E-5
2	0.0	0.0	0.0	0.0	8.134035897127221E-5
3	0.0	0.0	0.0	0.0	8.134035897127221E-5
4	0.0	0.0	0.0	0.0	8.134035897127221E-5
5	0.0	0.0	0.0	0.0	8.134035897127221E-5
6	0.0	0.0	0.0	0.0	8.134035897127221E-5
7	0.0	0.0	0.0	0.0	8.134035897127221E-5
8	0.0	0.0	0.0	0.0	1.2201053845690831E-4
9	0.0	0.0	0.0	8.134035897127221E-5	1.6268071794254443E-4
10	0.0	0.0	0.0	8.134035897127221E-5	2.4402107691381662E-4
11	0.0	0.0	0.0	8.134035897127221E-5	2.4402107691381662E-4
12	0.0	0.0	0.0	8.134035897127221E-5	3.2536143588508885E-4
13	0.0	0.0	0.0	8.134035897127221E-5	4.473719743419972E-4
14	0.0	0.0	0.0	8.134035897127221E-5	4.473719743419972E-4
15	0.0	0.0	0.0	8.134035897127221E-5	5.287123333132694E-4
16	0.0	0.0	0.0	8.134035897127221E-5	6.507228717701777E-4
17	0.0	0.0	0.0	8.134035897127221E-5	6.507228717701777E-4
18	0.0	0.0	0.0	1.2201053845690831E-4	7.3206323074145E-4
19	0.0	0.0	0.0	1.2201053845690831E-4	7.3206323074145E-4
20	0.0	0.0	0.0	2.0335089742818052E-4	7.72733410227086E-4
21	0.0	0.0	0.0	2.846912563994527E-4	8.134035897127221E-4
22	0.0	0.0	0.0	4.0670179485636105E-4	8.947439486839944E-4
23	0.0	0.0	0.0	9.354141281696305E-4	0.0010980948461121749
24	0.0	0.0	0.0	0.0020335089742818052	0.0010980948461121749
25	0.0	0.0	0.0	0.002277530051195622	0.0010980948461121749
26	0.0	0.0	0.0	0.0025215511281094388	0.0010980948461121749
27	0.0	0.0	0.0	0.003009593281937072	0.0010980948461121749
28	0.0	0.0	0.0	0.006141197102331052	0.0010980948461121749
29	0.0	0.0	0.0	0.010899608102150477	0.0010980948461121749
30	0.0	0.0	0.0	0.022124577640186043	0.0010980948461121749
31	0.0	0.0	0.0	0.049739629510932956	0.0010980948461121749
32	0.0	0.0	0.0	0.07645993743299588	0.001138765025597811
33	0.0	0.0	0.0	0.10415632966271407	0.001138765025597811
34	0.0	0.0	0.0	0.1355130380461395	0.0012201053845690831
35	0.0	0.0	0.0	0.17236022066012582	0.0012201053845690831
36	0.0	0.0	0.0	0.2264108891965362	0.0012201053845690831
37	0.0	0.0	0.0	0.30193541250136247	0.0012201053845690831
38	0.0	0.0	0.0	0.4062950930615047	0.0012607755640547194
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4970	0.0	22.752895	1
CGACTAT	55	0.004485532	19.99952	14
AACGCAG	6260	0.0	17.8171	6
GTCCTAT	830	0.0	17.229202	1
TAACGGC	230	0.0	17.219078	36
TTAACGG	220	1.8189894E-12	17.001665	35
ATACCGT	680	0.0	16.822783	6
TACCGTC	670	0.0	16.745525	7
CTATTCC	790	0.0	16.708118	4
CCGTCGT	690	0.0	16.260479	9
GTATTAG	545	0.0	16.147058	1
GGTATCA	2475	0.0	16.089157	1
ACCGTCG	700	0.0	16.02786	8
TCAACGC	6980	0.0	16.010752	4
TATCAAC	7100	0.0	15.894747	2
ATCAACG	7070	0.0	15.83773	3
CAACGCA	7140	0.0	15.682777	5
CGTCGTA	705	0.0	15.602462	10
CCTATTC	875	0.0	15.336151	3
ACGCAGA	7515	0.0	14.841657	7
>>END_MODULE