FastQCFastQC Report
Thu 2 Feb 2017
SRR4064071_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064071_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870938
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87760.30568406562593825No Hit
TATCAACGCAGAGTACTTTTTTTTT61780.21519099332691963No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41980.14622398672489617No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG30290.10550558737248941No Hit
GGTATCAACGCAGAGTACTTTTTTT30040.10463479183458507No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28810.10035047778809575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA2350.012.1282949
CGTCGTA4900.011.82694310
CGTCTTA2300.011.5668315
GTATCAA49400.011.4777681
CCGTCGT4850.011.3614199
ACCGTCG4950.011.324228
TAGACTG2100.011.3081455
TACCGTA700.00149120110.85797
GTCTAGA2200.010.7926321
GTTCTAA2300.010.7363231
ATCGCCA5050.010.72445416
ATTAGAC803.7831147E-410.6843343
GTTTTCG4450.010.67564615
AGAACCG3650.010.669935
CGAACGT2050.010.6564934
ACGCGCG1252.239558E-710.64092713
CCCTTAA2500.010.6372181
GCATCGC5300.010.57657814
AACCGCG2700.010.5562917
GAACCGT909.5273295E-510.5548196
Produced by FastQC (version 0.11.5)