##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064071_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2870938
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.295791828315345	32.0	32.0	32.0	32.0	32.0
2	30.932789562157037	32.0	32.0	32.0	32.0	32.0
3	30.948384813604473	32.0	32.0	32.0	32.0	32.0
4	30.97714753853967	32.0	32.0	32.0	32.0	32.0
5	30.89325265819046	32.0	32.0	32.0	32.0	32.0
6	34.55194121224492	36.0	36.0	36.0	32.0	36.0
7	34.499137912417474	36.0	36.0	36.0	32.0	36.0
8	34.4630883704211	36.0	36.0	36.0	32.0	36.0
9	34.58484369916731	36.0	36.0	36.0	32.0	36.0
10	34.31184860139787	36.0	36.0	36.0	32.0	36.0
11	34.55955823497408	36.0	36.0	36.0	32.0	36.0
12	34.39372602264486	36.0	36.0	36.0	32.0	36.0
13	34.478753285511566	36.0	36.0	36.0	32.0	36.0
14	34.37572284737602	36.0	36.0	36.0	32.0	36.0
15	34.31711726272041	36.0	36.0	36.0	32.0	36.0
16	34.31789540561308	36.0	36.0	36.0	32.0	36.0
17	34.23610819878381	36.0	36.0	36.0	32.0	36.0
18	34.23724371616524	36.0	36.0	36.0	32.0	36.0
19	34.25228340005949	36.0	36.0	36.0	32.0	36.0
20	34.232523307713365	36.0	36.0	36.0	32.0	36.0
21	34.21175274422506	36.0	36.0	36.0	32.0	36.0
22	34.1968875677566	36.0	36.0	36.0	32.0	36.0
23	34.14957654954583	36.0	36.0	36.0	32.0	36.0
24	34.13311398574264	36.0	36.0	36.0	32.0	36.0
25	33.74497011081396	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	4.0
4	35.0
5	33.0
6	110.0
7	38.0
8	154.0
9	188.0
10	264.0
11	79.0
12	205.0
13	153.0
14	269.0
15	471.0
16	763.0
17	1065.0
18	1363.0
19	1835.0
20	2726.0
21	3865.0
22	5796.0
23	8901.0
24	13312.0
25	19703.0
26	28210.0
27	37821.0
28	52894.0
29	72322.0
30	96013.0
31	132191.0
32	189477.0
33	272916.0
34	595300.0
35	1332460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.72912212917189	17.003365771439068	11.224650869598397	26.04286122979065
2	17.011889352251146	19.72645990461209	36.83072755924821	26.430923183888556
3	18.612170114790384	23.96616374393262	28.453003168012653	28.96866297326434
4	12.721670269588667	15.217404934760756	35.45445932135022	36.60646547430035
5	14.86295035139819	36.28014317190208	33.236190094664146	15.620716382035585
6	34.36889706696914	35.28614416908839	16.74555990393465	13.599398860007817
7	30.444527130745858	30.306143708327323	20.809320046852477	18.440009114074346
8	28.544185326936855	32.13704810461087	19.38711012741854	19.93165644103373
9	27.77838570235734	13.889593616745449	18.367295020609806	39.96472566028741
10	16.10542707417006	26.16100268921012	31.28430346112077	26.44926677549905
11	37.35375946518544	20.98161557519854	22.500262256254157	19.164362703361864
12	25.14082941046484	23.408702598817598	28.494977822112638	22.95549016860492
13	29.317999067981905	19.26579699223748	25.341586027117753	26.074617912662863
14	24.05942495598584	19.381706909430342	24.95595098298584	31.602917151597982
15	25.325629929931882	26.65282544869525	22.30479630615835	25.71674831521451
16	25.97337322655483	25.43447315294013	23.396475138306418	25.195678482198623
17	24.226505530263264	25.492781415359822	24.957426000009757	25.323287054367157
18	24.98475788833746	24.656525244736546	25.881471622830855	24.47724524409514
19	25.734161745298177	24.53961297860904	25.323987671422927	24.402237604669857
20	25.951494611964915	24.00202044779621	24.68467105499376	25.36181388524512
21	27.172098146592443	23.70172582674805	24.437197823457442	24.68897820320207
22	26.193384436798677	23.726021284976767	24.837016199031613	25.24357807919294
23	24.70445101374906	23.79995537830828	25.442940011713194	26.05265359622947
24	24.85320010694736	24.73234401215045	25.105230555572017	25.309225325330175
25	25.057133813736097	24.204030809731503	25.1516364183966	25.587198958135797
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	277.0
1	277.0
2	762.0
3	1247.0
4	1247.0
5	1247.0
6	3511.5
7	5776.0
8	5776.0
9	5776.0
10	5910.5
11	6045.0
12	6045.0
13	6045.0
14	6653.5
15	7262.0
16	7262.0
17	7262.0
18	12231.0
19	17200.0
20	17200.0
21	17200.0
22	27809.0
23	38418.0
24	38418.0
25	38418.0
26	57142.0
27	75866.0
28	75866.0
29	75866.0
30	94208.0
31	112550.0
32	112550.0
33	112550.0
34	140067.5
35	167585.0
36	167585.0
37	167585.0
38	195392.0
39	223199.0
40	223199.0
41	223199.0
42	258605.0
43	294011.0
44	294011.0
45	294011.0
46	331593.5
47	369176.0
48	369176.0
49	369176.0
50	382391.0
51	395606.0
52	395606.0
53	395606.0
54	370692.5
55	345779.0
56	345779.0
57	345779.0
58	318454.0
59	291129.0
60	291129.0
61	291129.0
62	257049.5
63	222970.0
64	222970.0
65	222970.0
66	183725.0
67	144480.0
68	144480.0
69	144480.0
70	108891.5
71	73303.0
72	73303.0
73	73303.0
74	56089.0
75	38875.0
76	38875.0
77	38875.0
78	30976.0
79	23077.0
80	23077.0
81	23077.0
82	15698.0
83	8319.0
84	8319.0
85	8319.0
86	6430.0
87	4541.0
88	4541.0
89	4541.0
90	3217.5
91	1894.0
92	1894.0
93	1894.0
94	1359.0
95	824.0
96	824.0
97	824.0
98	1176.5
99	1529.0
100	1529.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04057907206634208
2	0.0046674640831672436
3	0.0018460865403571933
4	0.004179818581940815
5	0.009160769058753621
6	0.013654074034339997
7	0.022710347628545097
8	0.03573744887559397
9	0.04980950476812805
10	0.05900510564839784
11	0.05597473717649075
12	0.06492651530614733
13	0.0660063017731487
14	0.06879284749444257
15	0.06025905122298009
16	0.06778272467047355
17	0.063254587873371
18	0.07805811201774472
19	0.07509740718886998
20	0.07896373937716523
21	0.07551538904706406
22	0.07830193476835794
23	0.08283007156546049
24	0.07791878473168003
25	0.0778142892671315
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2870938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.47506106340436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.10069356051733	28.659554849460484
2	15.44530004138778	12.8118952471832
3	6.18468192314293	7.695301812602586
4	2.925676811690253	4.853704976665576
5	1.6472841942096745	3.4160606271813547
6	1.0612582267424464	2.6409449854909917
7	0.6891124626544893	2.000668702770353
8	0.4829874771456381	1.6025548085979966
9	0.36652490888764644	1.3681478679636472
>10	1.8747539378408715	13.690144367348985
>50	0.10934198413450952	3.1368980776454136
>100	0.08981367741501058	7.918334482629224
>500	0.014654172374252894	4.258111582536901
>1k	0.007748183094418306	5.425333795435168
>5k	1.6843876292213712E-4	0.5223438164881373
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8776	0.30568406562593825	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6178	0.21519099332691963	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4198	0.14622398672489617	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3029	0.10550558737248941	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3004	0.10463479183458507	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2881	0.10035047778809575	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3932728606469384E-4
2	0.0	0.0	0.0	0.0	1.3932728606469384E-4
3	0.0	0.0	0.0	0.0	1.3932728606469384E-4
4	0.0	0.0	0.0	0.0	1.3932728606469384E-4
5	0.0	0.0	0.0	0.0	1.3932728606469384E-4
6	0.0	0.0	0.0	0.0	1.3932728606469384E-4
7	0.0	0.0	0.0	0.0	1.3932728606469384E-4
8	0.0	0.0	0.0	0.0	1.3932728606469384E-4
9	0.0	0.0	0.0	0.0	1.3932728606469384E-4
10	0.0	0.0	0.0	0.0	1.3932728606469384E-4
11	0.0	0.0	0.0	3.483182151617346E-5	1.3932728606469384E-4
12	0.0	0.0	0.0	3.483182151617346E-5	1.741591075808673E-4
13	0.0	0.0	0.0	3.483182151617346E-5	2.786545721293877E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGTA	235	0.0	12.128294	9
CGTCGTA	490	0.0	11.826943	10
CGTCTTA	230	0.0	11.56683	15
GTATCAA	4940	0.0	11.477768	1
CCGTCGT	485	0.0	11.361419	9
ACCGTCG	495	0.0	11.32422	8
TAGACTG	210	0.0	11.308145	5
TACCGTA	70	0.001491201	10.8579	7
GTCTAGA	220	0.0	10.792632	1
GTTCTAA	230	0.0	10.736323	1
ATCGCCA	505	0.0	10.724454	16
ATTAGAC	80	3.7831147E-4	10.684334	3
GTTTTCG	445	0.0	10.675646	15
AGAACCG	365	0.0	10.66993	5
CGAACGT	205	0.0	10.656493	4
ACGCGCG	125	2.239558E-7	10.640927	13
CCCTTAA	250	0.0	10.637218	1
GCATCGC	530	0.0	10.576578	14
AACCGCG	270	0.0	10.556291	7
GAACCGT	90	9.5273295E-5	10.554819	6
>>END_MODULE