FastQCFastQC Report
Thu 2 Feb 2017
SRR4064071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870938
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA30510.10627188744584523No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA29980.10442580090548803No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT29890.10411231451184248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61150.020.255241
ATACCGT9750.018.501076
ACCGTCG9600.018.332178
TTACGCC750.001292306717.5982744
CCGTCGT9850.017.4208249
TACCGTC9600.017.415267
GTCTTAG4550.017.4067021
AACGCAG71350.017.3580936
CGTCGTA9650.017.0979610
GTATTAG7350.017.0613331
GTATTAT2900.016.689761
CGTAAGA800.00199055316.498382
CGCAATA8750.016.09237136
ATACGAA8900.015.5725940
GTCGTAG10800.015.27734111
TCTAGCG8850.015.16420128
CAATACG9000.015.15565138
TACGAAT9450.015.13184441
CGAATGC9350.015.05839443
GTATATA3800.015.0527271
Produced by FastQC (version 0.11.5)