##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064071_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2870938
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32159141019416	32.0	32.0	32.0	32.0	32.0
2	31.421221914231516	32.0	32.0	32.0	32.0	32.0
3	31.50062279296871	32.0	32.0	32.0	32.0	32.0
4	31.581129233720826	32.0	32.0	32.0	32.0	32.0
5	31.530079019470293	32.0	32.0	32.0	32.0	32.0
6	35.13540940278055	36.0	36.0	36.0	36.0	36.0
7	35.152582535742674	36.0	36.0	36.0	36.0	36.0
8	35.1006103231766	36.0	36.0	36.0	36.0	36.0
9	35.205711164783075	36.0	36.0	36.0	36.0	36.0
10	35.06625500097877	36.0	36.0	36.0	36.0	36.0
11	35.20947892291648	36.0	36.0	36.0	36.0	36.0
12	35.10838583069366	36.0	36.0	36.0	36.0	36.0
13	35.15662233040212	36.0	36.0	36.0	36.0	36.0
14	35.09770674253502	36.0	36.0	36.0	36.0	36.0
15	35.06844940573429	36.0	36.0	36.0	36.0	36.0
16	35.082445876574134	36.0	36.0	36.0	36.0	36.0
17	35.038709996523785	36.0	36.0	36.0	36.0	36.0
18	35.042192133720754	36.0	36.0	36.0	36.0	36.0
19	35.03734145425641	36.0	36.0	36.0	36.0	36.0
20	35.035151229319474	36.0	36.0	36.0	36.0	36.0
21	35.030586170791565	36.0	36.0	36.0	36.0	36.0
22	35.0205981459718	36.0	36.0	36.0	36.0	36.0
23	34.96618700926317	36.0	36.0	36.0	36.0	36.0
24	34.938434407151945	36.0	36.0	36.0	36.0	36.0
25	34.913063604996	36.0	36.0	36.0	32.0	36.0
26	34.8515474733345	36.0	36.0	36.0	32.0	36.0
27	34.85167635107411	36.0	36.0	36.0	32.0	36.0
28	34.826969095118045	36.0	36.0	36.0	32.0	36.0
29	34.80052582117761	36.0	36.0	36.0	32.0	36.0
30	34.78014607072671	36.0	36.0	36.0	32.0	36.0
31	34.776703293488055	36.0	36.0	36.0	32.0	36.0
32	34.734461698580745	36.0	36.0	36.0	32.0	36.0
33	34.706611219051055	36.0	36.0	36.0	32.0	36.0
34	34.695319439151945	36.0	36.0	36.0	32.0	36.0
35	34.65076187643202	36.0	36.0	36.0	32.0	36.0
36	34.629567409675865	36.0	36.0	36.0	32.0	36.0
37	34.618674802451324	36.0	36.0	36.0	32.0	36.0
38	34.583451471261306	36.0	36.0	36.0	32.0	36.0
39	34.57895119992142	36.0	36.0	36.0	32.0	36.0
40	34.55986858650378	36.0	36.0	36.0	32.0	36.0
41	34.54069854521414	36.0	36.0	36.0	32.0	36.0
42	34.464917737687124	36.0	36.0	36.0	32.0	36.0
43	34.45776537145699	36.0	36.0	36.0	32.0	36.0
44	34.3874918232299	36.0	36.0	36.0	32.0	36.0
45	34.354919890293694	36.0	36.0	36.0	32.0	36.0
46	34.325982657932705	36.0	36.0	36.0	32.0	36.0
47	34.29084187815968	36.0	36.0	36.0	32.0	36.0
48	34.24360017527373	36.0	36.0	36.0	32.0	36.0
49	34.21609766564099	36.0	36.0	36.0	32.0	36.0
50	33.61445388231999	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	9.0
19	10.0
20	22.0
21	94.0
22	284.0
23	826.0
24	2150.0
25	4900.0
26	9874.0
27	18710.0
28	30709.0
29	47888.0
30	69535.0
31	99270.0
32	144986.0
33	235278.0
34	496514.0
35	1709877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.845301996706695	17.930369735083396	11.766806227241865	26.457522040968033
2	16.197323162395392	20.388585560403005	36.53219781081011	26.881893466391492
3	17.974112212458056	24.43376342640309	28.750519431166705	28.841604929972142
4	12.334818794414927	15.849558576325926	35.697461944493405	36.11816068476574
5	14.508219961559602	36.664741627997536	33.07337183875096	15.753666571691898
6	34.43079305577664	35.57421925206893	16.511496436872612	13.483491255281821
7	30.34922793391556	30.496427468004306	20.797594370889186	18.35675022719095
8	28.519320166440377	32.33427541799927	19.283000886818176	19.863403528742175
9	27.802288059788832	13.74100283395056	18.275010240676803	40.181698865583805
10	16.023624556804442	26.43575506172083	31.35273089273753	26.1878894887372
11	37.45522803182376	20.940027593778616	22.48816327213032	19.116581102267308
12	25.149202315344066	23.46511623046716	28.44036122042513	22.945320233763645
13	29.370050380922123	19.249385564528264	25.453860215233544	25.92670383931607
14	24.066921915143812	19.29094007233898	24.989898736575768	31.652239275941437
15	25.365552050912726	26.76847354130128	22.238857180676433	25.627117227109565
16	26.065721092890083	25.4829114012233	23.30472461789252	25.14664288799409
17	24.248137716662637	25.54980288672204	24.9161075578783	25.285951838737024
18	25.160549188467694	24.58265222348043	25.91316323960867	24.343635348443204
19	25.800626833460004	24.567232033572303	25.38233148887228	24.249809644095414
20	26.06506836312693	23.94031318891268	24.755174214249863	25.23944423371053
21	27.251093545036497	23.752620223773555	24.43208456608955	24.564201665100395
22	26.295117689840524	23.69572153471899	24.93489928023474	25.074261495205747
23	24.74651791956078	23.691243865627413	25.50537059116734	26.056867623644465
24	25.03532840877782	24.659908551930585	25.17152173223975	25.13324130705185
25	25.2166234743354	24.05499359081536	25.29962938193167	25.42875355291757
26	25.093319975699885	24.78030269251991	25.48582818256299	24.640549149217215
27	25.558511805609257	24.43396403871596	24.775163598134846	25.232360557539934
28	24.927120151343466	24.238118642715794	25.667187191399254	25.167574014541483
29	24.847144733638398	24.4509558657305	25.475738395522168	25.22616100510893
30	24.830171538763246	24.66513261981345	25.611999005168524	24.89269683625478
31	25.39583233910064	24.430814118951915	24.613276864399666	25.560076677547784
32	25.105857666547305	24.540586783780054	24.533585338904764	25.819970210767874
33	24.779356312950547	24.274341169114585	25.103446594247952	25.84285592368691
34	25.561008437550182	24.184729507599993	25.18457032168443	25.069691733165396
35	25.999540901400735	24.111767867487004	25.194341871011407	24.694349360100848
36	25.041809966604276	24.724817093370426	24.800512204748134	25.432860735277163
37	25.856384536956178	24.548465089578965	24.775891479051797	24.819258894413057
38	24.97600758560894	24.369639589521576	25.20316480411217	25.451188020757314
39	25.558476536687845	24.448716577707756	24.554401367423235	25.438405518181163
40	25.65037776005183	24.62685527383685	25.102704058351648	24.620062907759667
41	24.772322814116144	25.05262395235403	25.311781460554588	24.86327177297524
42	26.01119726745711	25.016763158600554	24.756773099806715	24.21526647413562
43	25.26823535394736	24.165965084731475	25.067879576145817	25.497919985175344
44	25.016101250655282	24.63806582964546	24.67321166395845	25.672621255740808
45	25.125640218245454	24.947298683059227	24.71737046552714	25.20969063316818
46	25.037618865164234	24.718520324636874	24.78264655682887	25.461214253370024
47	25.12397822678654	24.525901060550698	25.197823514638607	25.152297198024147
48	25.855708397498038	25.135542677525642	24.15597796763138	24.85277095734494
49	24.93788587302002	25.142732742931283	24.45915515034353	25.460226233705164
50	24.94912741588172	25.31925550820684	24.499755825529153	25.231861250382288
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	53.0
1	54.5
2	56.0
3	212.5
4	369.0
5	443.0
6	517.0
7	518.5
8	520.0
9	707.0
10	894.0
11	1340.0
12	1786.0
13	3342.5
14	4899.0
15	6641.0
16	8383.0
17	9028.0
18	9673.0
19	9728.0
20	9783.0
21	10593.0
22	11403.0
23	12512.0
24	13621.0
25	14807.0
26	15993.0
27	19370.0
28	22747.0
29	26826.5
30	30906.0
31	34615.5
32	38325.0
33	44257.5
34	50190.0
35	58441.0
36	66692.0
37	81036.0
38	95380.0
39	105895.0
40	116410.0
41	129748.5
42	143087.0
43	146642.5
44	150198.0
45	166482.0
46	182766.0
47	196142.5
48	209519.0
49	218223.5
50	226928.0
51	221987.0
52	217046.0
53	215919.5
54	214793.0
55	216042.5
56	217292.0
57	211134.0
58	204976.0
59	188775.5
60	172575.0
61	151778.5
62	130982.0
63	112833.5
64	94685.0
65	80886.5
66	67088.0
67	57276.0
68	47464.0
69	41993.5
70	36523.0
71	28255.5
72	19988.0
73	17025.0
74	14062.0
75	10928.5
76	7795.0
77	6802.0
78	5809.0
79	4707.5
80	3606.0
81	2924.0
82	2242.0
83	1858.0
84	1474.0
85	1089.0
86	704.0
87	507.5
88	311.0
89	231.5
90	152.0
91	125.0
92	98.0
93	86.5
94	75.0
95	61.0
96	47.0
97	36.0
98	25.0
99	26.5
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009091105415721272
2	4.52813679710255E-4
3	3.134863936455611E-4
4	0.0
5	0.0
6	1.741591075808673E-4
7	1.741591075808673E-4
8	0.0
9	0.0011842819315498977
10	9.404591809366834E-4
11	3.483182151617346E-5
12	1.3932728606469384E-4
13	3.483182151617346E-4
14	3.483182151617346E-4
15	0.004980950476812805
16	6.269727872911222E-4
17	0.0
18	3.8315003667790807E-4
19	0.0
20	6.618046088072957E-4
21	0.0
22	6.966364303234692E-5
23	8.707955379043365E-4
24	6.618046088072957E-4
25	0.0020202456479380604
26	0.005921409657749488
27	0.006409055158975917
28	0.004075323117392295
29	0.002960704828874744
30	0.003483182151617346
31	0.007488841625977294
32	0.003552845794649693
33	0.00463263226165107
34	0.0028213775428100503
35	0.0033090230440364784
36	0.0073495143399126
37	0.0041449867604246415
38	0.008324805342365457
39	0.004771959547715764
40	0.0023685638630997954
41	0.0033786866870688255
42	0.002055077469454234
43	0.0015674319682278059
44	0.0014977683251954587
45	0.0014629365036792853
46	0.0013236092176145914
47	0.0024730593276483157
48	0.0024730593276483157
49	4.52813679710255E-4
50	0.0013932728606469384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2870938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.968627616853034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.72265055691192	31.803005369705957
2	15.162973838593619	13.63716248223602
3	5.690594442540064	7.676946672153526
4	2.7481907153771576	4.943294595995535
5	1.5202135049119057	3.418095750024723
6	0.9408247764828721	2.538455941582036
7	0.6132028102459973	1.9302422179292031
8	0.4427023909828204	1.592617517215754
9	0.33228204626382035	1.3448040841983302
>10	1.6419620676165876	13.010286616371694
>50	0.09090440519195181	2.8444725167138007
>100	0.07583565931062988	7.244390139891006
>500	0.011593634581751815	3.6062013101598227
>1k	0.006069150989058846	4.410024785822631
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	3051	0.10627188744584523	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2998	0.10442580090548803	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2989	0.10411231451184248	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	3.483182151617346E-5
8	0.0	0.0	0.0	0.0	3.483182151617346E-5
9	0.0	0.0	0.0	0.0	3.483182151617346E-5
10	0.0	0.0	0.0	0.0	3.483182151617346E-5
11	0.0	0.0	0.0	3.483182151617346E-5	3.483182151617346E-5
12	0.0	0.0	0.0	3.483182151617346E-5	1.741591075808673E-4
13	0.0	0.0	0.0	3.483182151617346E-5	2.786545721293877E-4
14	0.0	0.0	0.0	3.483182151617346E-5	2.786545721293877E-4
15	0.0	0.0	0.0	3.483182151617346E-5	3.8315003667790807E-4
16	0.0	0.0	0.0	6.966364303234692E-5	4.179818581940815E-4
17	0.0	0.0	0.0	6.966364303234692E-5	4.179818581940815E-4
18	0.0	0.0	0.0	1.0449546454852037E-4	4.179818581940815E-4
19	0.0	0.0	0.0	1.3932728606469384E-4	4.8764550122642846E-4
20	0.0	0.0	0.0	2.0899092909704075E-4	5.921409657749488E-4
21	0.0	0.0	0.0	3.483182151617346E-4	5.921409657749488E-4
22	0.0	0.0	0.0	4.8764550122642846E-4	5.921409657749488E-4
23	0.0	0.0	0.0	0.0010101228239690304	5.921409657749488E-4
24	0.0	0.0	0.0	0.001741591075808673	5.921409657749488E-4
25	0.0	0.0	0.0	0.002020245647938061	5.921409657749488E-4
26	0.0	0.0	0.0	0.002577554792196836	6.269727872911223E-4
27	0.0	0.0	0.0	0.0031348639364556113	6.966364303234692E-4
28	0.0	0.0	0.0	0.005398932335006886	6.966364303234692E-4
29	0.0	0.0	0.0	0.010937191956078467	6.966364303234692E-4
30	0.0	0.0	0.0	0.021595729340027545	6.966364303234692E-4
31	0.0	0.0	0.0	0.04740610908351208	6.966364303234692E-4
32	0.0	0.0	0.0	0.07440077075854651	6.966364303234692E-4
33	0.0	0.0	0.0	0.09958417771473992	6.966364303234692E-4
34	0.0	0.0	0.0	0.12884290778832563	7.663000733558161E-4
35	0.0	0.0	0.0	0.16315225198175648	7.663000733558161E-4
36	0.0	0.0	0.0	0.21160331571075378	7.663000733558161E-4
37	0.0	0.0	0.0	0.2843321590365239	7.663000733558161E-4
38	0.0	0.0	0.0	0.3807814728148083	9.0562735942051E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6115	0.0	20.25524	1
ATACCGT	975	0.0	18.50107	6
ACCGTCG	960	0.0	18.33217	8
TTACGCC	75	0.0012923067	17.598274	4
CCGTCGT	985	0.0	17.420824	9
TACCGTC	960	0.0	17.41526	7
GTCTTAG	455	0.0	17.406702	1
AACGCAG	7135	0.0	17.358093	6
CGTCGTA	965	0.0	17.09796	10
GTATTAG	735	0.0	17.061333	1
GTATTAT	290	0.0	16.68976	1
CGTAAGA	80	0.001990553	16.49838	2
CGCAATA	875	0.0	16.092371	36
ATACGAA	890	0.0	15.57259	40
GTCGTAG	1080	0.0	15.277341	11
TCTAGCG	885	0.0	15.164201	28
CAATACG	900	0.0	15.155651	38
TACGAAT	945	0.0	15.131844	41
CGAATGC	935	0.0	15.058394	43
GTATATA	380	0.0	15.052727	1
>>END_MODULE