FastQCFastQC Report
Thu 2 Feb 2017
SRR4064070_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064070_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077385
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36560.33934016159497304No Hit
TATCAACGCAGAGTACTTTTTTTTT25860.24002561758331517No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22570.20948871573300168No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15050.13969008293228513No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12810.11889900082143337No Hit
GGTATCAACGCAGAGTACTTTTTTT12620.11713547153524506No Hit
GTACATGGGGTGGTATCAACGCAAA12140.11268023965434826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCT551.1296272E-515.5426044
CCTAATA551.1326138E-515.5382752
CGAACGA1006.566552E-1014.25268416
TAGGACG400.00528413114.2473884
TGAGTAA551.9584593E-413.81885810
TCAATAC551.9646414E-413.81372453
GTATAAT852.70702E-713.4074371
CGGAGTA500.001497424313.30188614
CGAGACT655.4588323E-513.1514364
CGAACGT604.0965414E-412.6643454
GTGCAAT751.4824938E-512.6625811
GTATTAG1800.012.6625811
TCTATAC604.1013584E-412.6625813
CGTTATT751.4846186E-512.6608172
ATATACG1750.012.4845866
ACGAACG1301.9645086E-1012.42541615
GTATTAC1001.4468787E-712.3460161
GCGTTAT853.9543756E-612.2901511
TTAATAT2250.012.2404953
CAAGACG1800.012.1366634
Produced by FastQC (version 0.11.5)