##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064070_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1077385
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26907558579338	32.0	32.0	32.0	32.0	32.0
2	30.92187472444855	32.0	32.0	32.0	32.0	32.0
3	30.94114267416012	32.0	32.0	32.0	32.0	32.0
4	30.95548852081661	32.0	32.0	32.0	32.0	32.0
5	30.88166904124338	32.0	32.0	32.0	32.0	32.0
6	34.539990811084245	36.0	36.0	36.0	32.0	36.0
7	34.485165470096575	36.0	36.0	36.0	32.0	36.0
8	34.4317880794702	36.0	36.0	36.0	32.0	36.0
9	34.554550137601694	36.0	36.0	36.0	32.0	36.0
10	34.296012103379944	36.0	36.0	36.0	32.0	36.0
11	34.539904490966556	36.0	36.0	36.0	32.0	36.0
12	34.3844001912037	36.0	36.0	36.0	32.0	36.0
13	34.44861307703374	36.0	36.0	36.0	32.0	36.0
14	34.35396817293725	36.0	36.0	36.0	32.0	36.0
15	34.30054715816537	36.0	36.0	36.0	32.0	36.0
16	34.29916510810899	36.0	36.0	36.0	32.0	36.0
17	34.236678624632795	36.0	36.0	36.0	32.0	36.0
18	34.22286833397532	36.0	36.0	36.0	32.0	36.0
19	34.23018326781977	36.0	36.0	36.0	32.0	36.0
20	34.20500656682616	36.0	36.0	36.0	32.0	36.0
21	34.19024675487407	36.0	36.0	36.0	32.0	36.0
22	34.17581737261982	36.0	36.0	36.0	32.0	36.0
23	34.12412925741494	36.0	36.0	36.0	32.0	36.0
24	34.1072931217717	36.0	36.0	36.0	32.0	36.0
25	33.730633895961056	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	11.0
5	17.0
6	50.0
7	16.0
8	74.0
9	77.0
10	94.0
11	27.0
12	70.0
13	52.0
14	124.0
15	178.0
16	318.0
17	414.0
18	537.0
19	703.0
20	1058.0
21	1527.0
22	2349.0
23	3685.0
24	5554.0
25	7849.0
26	11036.0
27	15126.0
28	20171.0
29	27402.0
30	35815.0
31	49187.0
32	69405.0
33	99711.0
34	218483.0
35	506262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.85127031850351	16.277644585663165	10.799703614014824	26.071381481818502
2	17.141690220594718	19.53161331643948	36.675902591164146	26.650793871801653
3	19.35228767330542	23.64258591131478	27.95748128992975	29.047645125450057
4	13.115963821871729	14.846064737463522	34.77624436573177	37.261727074932985
5	15.03990628196726	36.242311612458856	33.150526235665204	15.567255869908678
6	34.88416350417004	34.43141900171555	16.71218557604474	13.972231918069678
7	30.66170647538921	30.045055792867757	20.577583141539062	18.71565459020396
8	28.984043121178498	31.922114460544215	19.22030892368833	19.873533494588962
9	27.96422472193924	13.604430770602313	17.93992197490233	40.491422532556115
10	16.205882325625886	26.340165925083888	31.38757242853733	26.066379320752898
11	37.808058368563	20.556882601621183	22.210757617409524	19.42430141240629
12	25.244640933239214	23.124883902366534	28.53568376862206	23.094791395772187
13	30.23817838673245	18.98628365927599	24.87043407834512	25.905103875646436
14	24.296168739312847	18.70991591456035	24.930083602456897	32.06383174366991
15	25.67257191947805	26.188116742900924	22.284427314309596	25.85488402331143
16	26.20324729786588	25.07781087429608	23.3103824923443	25.40855933549374
17	24.46149673814513	25.06780051416938	24.925698066663696	25.545004681021798
18	25.2628547808034	24.51388469594861	25.62558926937564	24.597671253872342
19	26.0550192835009	24.238691842967654	25.06390678526577	24.642382088265673
20	26.178088287068128	23.749966326400752	24.67424856782699	25.39769681870413
21	27.18052418036441	23.625828552745	24.269443123383766	24.924204143506824
22	26.487679997622017	23.482595198329467	24.621612533463626	25.408112270584894
23	25.120508173375704	23.477472472858373	25.155436732396147	26.246582621369775
24	25.175072011978926	24.31863802845553	25.005921655797138	25.5003683037684
25	25.390418899135746	23.95507442389293	24.937763344653593	25.716743332317733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	346.0
1	346.0
2	480.5
3	615.0
4	615.0
5	615.0
6	1732.5
7	2850.0
8	2850.0
9	2850.0
10	2774.5
11	2699.0
12	2699.0
13	2699.0
14	2525.0
15	2351.0
16	2351.0
17	2351.0
18	4069.5
19	5788.0
20	5788.0
21	5788.0
22	9046.5
23	12305.0
24	12305.0
25	12305.0
26	18651.5
27	24998.0
28	24998.0
29	24998.0
30	31623.5
31	38249.0
32	38249.0
33	38249.0
34	47657.0
35	57065.0
36	57065.0
37	57065.0
38	67549.0
39	78033.0
40	78033.0
41	78033.0
42	92070.5
43	106108.0
44	106108.0
45	106108.0
46	122427.5
47	138747.0
48	138747.0
49	138747.0
50	145564.5
51	152382.0
52	152382.0
53	152382.0
54	144453.0
55	136524.0
56	136524.0
57	136524.0
58	126598.5
59	116673.0
60	116673.0
61	116673.0
62	102246.0
63	87819.0
64	87819.0
65	87819.0
66	71667.5
67	55516.0
68	55516.0
69	55516.0
70	42268.0
71	29020.0
72	29020.0
73	29020.0
74	21912.0
75	14804.0
76	14804.0
77	14804.0
78	11497.0
79	8190.0
80	8190.0
81	8190.0
82	5690.5
83	3191.0
84	3191.0
85	3191.0
86	2419.5
87	1648.0
88	1648.0
89	1648.0
90	1155.5
91	663.0
92	663.0
93	663.0
94	441.0
95	219.0
96	219.0
97	219.0
98	400.5
99	582.0
100	582.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03814792298017886
2	0.006497213159641168
3	0.0022276159404484006
4	0.0045480492117488175
5	0.010302723724573852
6	0.016428667560806954
7	0.025617583315156605
8	0.03898327895784701
9	0.05123516663031321
10	0.05884618776017858
11	0.059495909076142696
12	0.06543621825067177
13	0.0661787568974879
14	0.06887045949219639
15	0.06125943836233101
16	0.06831355550708428
17	0.06469367960385564
18	0.07796655791569403
19	0.07583175930609763
20	0.08112234716466259
21	0.0750892206592815
22	0.07824500990825006
23	0.08372123242851906
24	0.07685274994546981
25	0.07880191389336218
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1077385.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.2082957579609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.87250404547868	38.56757539587514
2	14.740291816772299	15.391310294573978
3	5.001158019430677	7.833058110322042
4	2.1452797531774195	4.480055993498083
5	1.197180643868095	3.1251380565385776
6	0.7080556052651603	2.21798258716593
7	0.46271367770786415	1.6910244775918177
8	0.30885465906301224	1.2899820309268737
9	0.24020754941885802	1.1286744105019275
>10	1.1642884425168107	10.685941109839279
>50	0.0828768566016148	3.0371241015915698
>100	0.06961003347950931	7.284697972547471
>500	0.005726274642219284	1.981818161844418
>1k	0.0012526225779854684	1.2856172971829083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3656	0.33934016159497304	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2586	0.24002561758331517	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2257	0.20948871573300168	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1505	0.13969008293228513	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1281	0.11889900082143337	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1262	0.11713547153524506	No Hit
GTACATGGGGTGGTATCAACGCAAA	1214	0.11268023965434826	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8563466170403337E-4
2	0.0	0.0	0.0	0.0	1.8563466170403337E-4
3	0.0	0.0	0.0	0.0	1.8563466170403337E-4
4	0.0	0.0	0.0	0.0	1.8563466170403337E-4
5	0.0	0.0	0.0	0.0	1.8563466170403337E-4
6	0.0	0.0	0.0	0.0	1.8563466170403337E-4
7	0.0	0.0	0.0	0.0	1.8563466170403337E-4
8	0.0	0.0	0.0	0.0	1.8563466170403337E-4
9	0.0	0.0	0.0	0.0	1.8563466170403337E-4
10	0.0	0.0	0.0	9.281733085201668E-5	1.8563466170403337E-4
11	0.0	0.0	0.0	1.8563466170403337E-4	1.8563466170403337E-4
12	0.0	0.0	0.0	1.8563466170403337E-4	2.784519925560501E-4
13	0.0	0.0	0.0	1.8563466170403337E-4	4.6408665426008345E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAATCT	55	1.1296272E-5	15.542604	4
CCTAATA	55	1.1326138E-5	15.538275	2
CGAACGA	100	6.566552E-10	14.252684	16
TAGGACG	40	0.005284131	14.247388	4
TGAGTAA	55	1.9584593E-4	13.818858	10
TCAATAC	55	1.9646414E-4	13.8137245	3
GTATAAT	85	2.70702E-7	13.407437	1
CGGAGTA	50	0.0014974243	13.301886	14
CGAGACT	65	5.4588323E-5	13.151436	4
CGAACGT	60	4.0965414E-4	12.664345	4
GTGCAAT	75	1.4824938E-5	12.662581	1
GTATTAG	180	0.0	12.662581	1
TCTATAC	60	4.1013584E-4	12.662581	3
CGTTATT	75	1.4846186E-5	12.660817	2
ATATACG	175	0.0	12.484586	6
ACGAACG	130	1.9645086E-10	12.425416	15
GTATTAC	100	1.4468787E-7	12.346016	1
GCGTTAT	85	3.9543756E-6	12.290151	1
TTAATAT	225	0.0	12.240495	3
CAAGACG	180	0.0	12.136663	4
>>END_MODULE