FastQCFastQC Report
Thu 2 Feb 2017
SRR4064070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077385
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11030.1023775159297744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC1250.029.9178541
ACGATTA450.001397943624.4449640
TTAACGG602.8693836E-422.00352735
TGCGTAC500.002580636621.99944311
ACCGTCG3050.020.916538
TATACTA956.8696136E-720.8406095
TAAGGCT1800.020.7762874
TAGGCTA755.47495E-520.531865
GTCTAAT654.93407E-420.3062341
ATATAAG1101.2662713E-719.9985661
ATACCGT3000.019.7985786
TTAAGGG808.992365E-519.248623
GCTATAC1051.793549E-618.8557913
CGTCGTA3300.018.66619310
CCGTCGT3350.018.3875949
ATGTCGG600.007406723718.33542336
CGATTAA600.007410690718.3337241
TACCGTC3250.018.2756127
CTAATGC851.4314355E-418.1163483
GCACCGT1102.7977676E-617.9987096
Produced by FastQC (version 0.11.5)