FastQCFastQC Report
Thu 2 Feb 2017
SRR4064069_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064069_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431773
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69711.6145057703932388No Hit
TATCAACGCAGAGTACTTTTTTTTT51011.1814078230922267No Hit
GTACTTTTTTTTTTTTTTTTTTTTT46151.0688486774300385No Hit
GTACATGGGGTGGTATCAACGCAAA32880.7615112570725822No Hit
GAGTACTTTTTTTTTTTTTTTTTTT31830.7371929231332205No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27610.6394563810150241No Hit
GGTATCAACGCAGAGTACTTTTTTT24140.5590900774249432No Hit
GTATCAACGCAGAGTACATGGGGTG19790.4583426939618735No Hit
GTCCTACAGTGGACATTTCTAAATT18000.41688572467477125No Hit
GTCCTAAAGTGTGTATTTCTCATTT17810.4124852642476486No Hit
CTGTAGGACGTGGAATATGGCAAGA16500.3821452476185403No Hit
GTCCTACAGTGTGCATTTCTCATTT15410.35690050095767917No Hit
CTTTAGGACGTGAAATATGGCGAGG15170.3513420246286822No Hit
TATCAACGCAGAGTACATGGGGTGG14250.33003453203419386No Hit
GCAGAGTACTTTTTTTTTTTTTTTT14120.3270236906893206No Hit
GTACATGGGTGGTATCAACGCAAAA13760.3186859761958251No Hit
GTACATGGGAGTGGTATCAACGCAA10490.24295173621324168No Hit
GGTATCAACGCAGAGTACATGGGGT10270.23785646624499449No Hit
CTGTAGGACCTGGAATATGGCGAGA9890.2290555453907493No Hit
GATATACACTGTTCTACAAATCCCG9610.22257065634025286No Hit
CTGAAGGACCTGGAATATGGCGAGA8600.19917873512239068No Hit
ACGCAGAGTACATGGGGTGGTATCA8340.193157052432644No Hit
ATTTAGAAATGTCCACTGTAGGACG7840.18157689341390035No Hit
ATCAACGCAGAGTACTTTTTTTTTT7760.17972406797090137No Hit
TTGTAGAACAGTGTATATCAATGAG7140.16536467078765926No Hit
GTATTAGAGGCACTGCCTGCCCAGT7050.16328024216428538No Hit
GTATCAACGCAGAGTACATGGGAGT6670.15447932131004025No Hit
CCATAGGGTCTTCTCGTCTTATTAT6670.15447932131004025No Hit
GTCCTTCAGTGTGCATTTCTCATTT6490.15031046406329251No Hit
GTATCAACGCAGAGTACATGGGTGG6250.14475198773429557No Hit
GTGTATATCAATGAGTTACAATGAA6150.14243595593054684No Hit
CTCTAATACTTGTAATGCTAGAGGT5910.1368774796015499No Hit
GTGTATATCAATGAGTTACAATGAG5690.13178220963330267No Hit
TTTCTAAATTTTCCACCTTTTTCAG5610.1299293841903037No Hit
CTGTAGGACATGGAATATGGCAAGA5600.12969778100992885No Hit
GTATCAACGCAGAGTACATGGGAAG5600.12969778100992885No Hit
GAATATGGCAAGAAAACTGAAAATC5600.12969778100992885No Hit
GAGTACATGGGGTGGTATCAACGCA5540.1283081619276796No Hit
GTACATGGGGAATAATTGCAATCCC5480.12691854284543033No Hit
GTTCTACAGTGTGGTTTTTATCATT5220.12089686015568366No Hit
CCATTGGGATGTCCTGATCCAACAT5080.11765441563043542No Hit
GGAATATGGCGAGAAAACTGAAAAT4790.11093792339956413No Hit
CTTCTACACCATTGGGATGTCCTGA4690.10862189159581538No Hit
GGGTAGGCACACGCTGAGCCAGTCA4680.10839028841544052No Hit
GGTATCAACGCAGAGTACATGGGAG4640.10746387569394104No Hit
GTATCAACGCAGAGTACATGGGGAA4570.10584265343131691No Hit
GATATATTTTGATCAACGGACCAAG4540.1051478438901923No Hit
CAACGCAGAGTACTTTTTTTTTTTT4390.1016737961845692No Hit
GATATACACTGTTCTACAATGCCGG4340.10051578028269484No Hit
GTCAGGATACCGCGGCCGTTAAACT4340.10051578028269484No Hit
GTTCTACAAATCCCGTTTCCAACGA4330.10028417710231997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACCA250.00603707218.9935114
ATGTCGA350.002163342216.28958917
CGTCTTA951.8189894E-1116.00380715
CGACCTG755.8113073E-815.20009210
CTAGGAC704.43737E-714.9217443
CGTTTGG456.7361625E-414.78129515
AATCGTA653.3582874E-614.61547413
TGTCCAC1850.014.37846610
TAGGACC4450.014.2984874
GGTTCGT400.0052545214.25669718
TATGTCG400.005262612414.2533916
AGGGTAA400.005270715314.25008710
ACGAAAT801.2809687E-714.25008712
AATTCCG400.005282888714.2451335
CGAGACT400.005282888714.2451334
TAAGAGG400.005282888714.2451334
CTGTAGA1700.013.9690551
TTAGAAA1500.013.9269613
CTCGTCT1101.8371793E-1013.81826613
TCTCGTC1101.8371793E-1013.81826612
Produced by FastQC (version 0.11.5)