##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064069_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431773
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.043374643620606	32.0	32.0	32.0	32.0	32.0
2	30.8615476187719	32.0	32.0	32.0	32.0	32.0
3	30.90452390492226	32.0	32.0	32.0	32.0	32.0
4	30.866476134450277	32.0	32.0	32.0	32.0	32.0
5	30.90478098445248	32.0	32.0	32.0	32.0	32.0
6	34.46295159725133	36.0	36.0	36.0	32.0	36.0
7	34.29427963304792	36.0	36.0	36.0	32.0	36.0
8	34.23807648926635	36.0	36.0	36.0	32.0	36.0
9	34.25282961185623	36.0	36.0	36.0	32.0	36.0
10	34.113471662192865	36.0	36.0	36.0	32.0	36.0
11	34.428213436226905	36.0	36.0	36.0	32.0	36.0
12	34.24287067510011	36.0	36.0	36.0	32.0	36.0
13	34.30969282470187	36.0	36.0	36.0	32.0	36.0
14	34.26458347326025	36.0	36.0	36.0	32.0	36.0
15	34.24536504135275	36.0	36.0	36.0	32.0	36.0
16	34.23417397567703	36.0	36.0	36.0	32.0	36.0
17	34.15603569468216	36.0	36.0	36.0	32.0	36.0
18	34.171027368547826	36.0	36.0	36.0	32.0	36.0
19	34.085688544675094	36.0	36.0	36.0	32.0	36.0
20	34.02228717404748	36.0	36.0	36.0	32.0	36.0
21	33.97296264472304	36.0	36.0	36.0	32.0	36.0
22	33.96460408594331	36.0	36.0	36.0	32.0	36.0
23	34.042022081047214	36.0	36.0	36.0	32.0	36.0
24	34.0332396884474	36.0	36.0	36.0	32.0	36.0
25	33.71489880099033	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	3.0
5	4.0
6	20.0
7	5.0
8	24.0
9	25.0
10	38.0
11	14.0
12	29.0
13	20.0
14	134.0
15	134.0
16	178.0
17	246.0
18	336.0
19	394.0
20	605.0
21	803.0
22	1325.0
23	1810.0
24	2595.0
25	3553.0
26	5002.0
27	6390.0
28	8513.0
29	11169.0
30	15061.0
31	20123.0
32	28574.0
33	40805.0
34	89566.0
35	194275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.75301204819277	19.511816496756257	12.506719184430029	22.228452270620945
2	13.113356788645097	21.802090595562635	44.86419003898152	20.22036257681074
3	18.984920177224396	29.800194085263186	29.516709166243988	21.698176571268434
4	9.806537382777126	17.026702149095684	39.36372500654294	33.803035461584244
5	10.223958900333772	42.22675693892725	33.89008433492152	13.659199825817463
6	26.963239211507723	40.2703203539413	19.952282782423385	12.814157652127586
7	25.945574039368125	33.943136986904314	21.7029714897294	18.408317483998157
8	26.037870485996073	36.280231402431255	20.083219616846876	17.598678494725796
9	29.011268604319746	13.678041862760773	21.195774331310407	36.11491520160907
10	16.58683314678476	28.188068770378404	32.66361236828628	22.561485714550557
11	32.78049933493074	22.90271450221767	26.97215051421209	17.344635648639507
12	26.357065221169822	26.43377419751055	31.45299779143038	15.756162789889247
13	29.8025246988971	22.536425513966492	28.192084765363372	19.468965021773034
14	21.100996101807258	24.500210901034112	29.360900338832213	25.03789265832642
15	21.477236386557347	34.206135492512544	26.66098136363847	17.65564675729163
16	18.758574977751408	28.3243102936814	32.63682883417383	20.280285894393355
17	17.70622343556633	29.259927044853047	30.340580952866524	22.693268566714096
18	17.915187139002974	26.684575518491393	37.14317501228433	18.257062330221302
19	22.721636486264114	25.11536384516793	31.232255058974047	20.930744609593916
20	23.326642530328805	26.36369536294902	32.10797379924809	18.201688307474075
21	24.157049447072648	24.141287946392353	29.378741907744228	22.322920698790767
22	23.276285658659596	28.45762680437221	29.87933767835124	18.386749858616948
23	20.40128507651583	28.832629446425013	32.02144575743949	18.74463971961967
24	22.05438556640815	27.611383327384235	32.39839791580714	17.935833190400473
25	20.668850118328248	28.640998361252308	32.97406050154487	17.71609101887457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	329.0
1	329.0
2	735.5
3	1142.0
4	1142.0
5	1142.0
6	3324.5
7	5507.0
8	5507.0
9	5507.0
10	5007.5
11	4508.0
12	4508.0
13	4508.0
14	3259.5
15	2011.0
16	2011.0
17	2011.0
18	4006.0
19	6001.0
20	6001.0
21	6001.0
22	9956.5
23	13912.0
24	13912.0
25	13912.0
26	22349.0
27	30786.0
28	30786.0
29	30786.0
30	39133.5
31	47481.0
32	47481.0
33	47481.0
34	46993.0
35	46505.0
36	46505.0
37	46505.0
38	45487.5
39	44470.0
40	44470.0
41	44470.0
42	45087.0
43	45704.0
44	45704.0
45	45704.0
46	52117.5
47	58531.0
48	58531.0
49	58531.0
50	54262.0
51	49993.0
52	49993.0
53	49993.0
54	37141.5
55	24290.0
56	24290.0
57	24290.0
58	20794.5
59	17299.0
60	17299.0
61	17299.0
62	15056.5
63	12814.0
64	12814.0
65	12814.0
66	10930.5
67	9047.0
68	9047.0
69	9047.0
70	6946.5
71	4846.0
72	4846.0
73	4846.0
74	4127.0
75	3408.0
76	3408.0
77	3408.0
78	2600.5
79	1793.0
80	1793.0
81	1793.0
82	1214.0
83	635.0
84	635.0
85	635.0
86	471.0
87	307.0
88	307.0
89	307.0
90	223.0
91	139.0
92	139.0
93	139.0
94	99.5
95	60.0
96	60.0
97	60.0
98	157.5
99	255.0
100	255.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04006735020485301
2	0.006948095411246187
3	9.264127214994916E-4
4	0.002316031803748729
5	0.009727333575744663
6	0.014591000363616994
7	0.024086730758986784
8	0.0333508579739817
9	0.050952699682472034
10	0.057900795093718226
11	0.055353160109594624
12	0.06299606506196544
13	0.06392247778346492
14	0.06739652548908802
15	0.0581323982740931
16	0.06600690640683878
17	0.06369087460309006
18	0.07619744634333318
19	0.07226019227696034
20	0.07758706542558243
21	0.07920828768820654
22	0.0743446209003342
23	0.08314554175457937
24	0.07758706542558243
25	0.08013470040970602
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	431773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.01822472805316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.63908941998518	19.917845219180663
2	14.548054121506592	8.443174077075168
3	5.683774795204539	4.94799162932669
4	2.9847836071332394	3.4645248590560596
5	1.7042215196076345	2.47267415211793
6	1.0813056069154354	1.882654146070561
7	0.776570126197411	1.5774280507362353
8	0.5640033140824295	1.3093099932328554
9	0.44336319388925	1.1579051516783094
>10	2.887028393454014	16.729090132960238
>50	0.3546873641100608	7.180338218408666
>100	0.3003658759130245	16.946061816959766
>500	0.01837344453723288	3.565017641998608
>1k	0.012781526634596786	7.607565444769511
>5k	0.0015976908293245983	2.798419466428714
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6971	1.6145057703932388	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5101	1.1814078230922267	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4615	1.0688486774300385	No Hit
GTACATGGGGTGGTATCAACGCAAA	3288	0.7615112570725822	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3183	0.7371929231332205	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2761	0.6394563810150241	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2414	0.5590900774249432	No Hit
GTATCAACGCAGAGTACATGGGGTG	1979	0.4583426939618735	No Hit
GTCCTACAGTGGACATTTCTAAATT	1800	0.41688572467477125	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1781	0.4124852642476486	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1650	0.3821452476185403	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1541	0.35690050095767917	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1517	0.3513420246286822	No Hit
TATCAACGCAGAGTACATGGGGTGG	1425	0.33003453203419386	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1412	0.3270236906893206	No Hit
GTACATGGGTGGTATCAACGCAAAA	1376	0.3186859761958251	No Hit
GTACATGGGAGTGGTATCAACGCAA	1049	0.24295173621324168	No Hit
GGTATCAACGCAGAGTACATGGGGT	1027	0.23785646624499449	No Hit
CTGTAGGACCTGGAATATGGCGAGA	989	0.2290555453907493	No Hit
GATATACACTGTTCTACAAATCCCG	961	0.22257065634025286	No Hit
CTGAAGGACCTGGAATATGGCGAGA	860	0.19917873512239068	No Hit
ACGCAGAGTACATGGGGTGGTATCA	834	0.193157052432644	No Hit
ATTTAGAAATGTCCACTGTAGGACG	784	0.18157689341390035	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	776	0.17972406797090137	No Hit
TTGTAGAACAGTGTATATCAATGAG	714	0.16536467078765926	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	705	0.16328024216428538	No Hit
GTATCAACGCAGAGTACATGGGAGT	667	0.15447932131004025	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	667	0.15447932131004025	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	649	0.15031046406329251	No Hit
GTATCAACGCAGAGTACATGGGTGG	625	0.14475198773429557	No Hit
GTGTATATCAATGAGTTACAATGAA	615	0.14243595593054684	No Hit
CTCTAATACTTGTAATGCTAGAGGT	591	0.1368774796015499	No Hit
GTGTATATCAATGAGTTACAATGAG	569	0.13178220963330267	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	561	0.1299293841903037	No Hit
CTGTAGGACATGGAATATGGCAAGA	560	0.12969778100992885	No Hit
GTATCAACGCAGAGTACATGGGAAG	560	0.12969778100992885	No Hit
GAATATGGCAAGAAAACTGAAAATC	560	0.12969778100992885	No Hit
GAGTACATGGGGTGGTATCAACGCA	554	0.1283081619276796	No Hit
GTACATGGGGAATAATTGCAATCCC	548	0.12691854284543033	No Hit
GTTCTACAGTGTGGTTTTTATCATT	522	0.12089686015568366	No Hit
CCATTGGGATGTCCTGATCCAACAT	508	0.11765441563043542	No Hit
GGAATATGGCGAGAAAACTGAAAAT	479	0.11093792339956413	No Hit
CTTCTACACCATTGGGATGTCCTGA	469	0.10862189159581538	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	468	0.10839028841544052	No Hit
GGTATCAACGCAGAGTACATGGGAG	464	0.10746387569394104	No Hit
GTATCAACGCAGAGTACATGGGGAA	457	0.10584265343131691	No Hit
GATATATTTTGATCAACGGACCAAG	454	0.1051478438901923	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	439	0.1016737961845692	No Hit
GATATACACTGTTCTACAATGCCGG	434	0.10051578028269484	No Hit
GTCAGGATACCGCGGCCGTTAAACT	434	0.10051578028269484	No Hit
GTTCTACAAATCCCGTTTCCAACGA	433	0.10028417710231997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.316031803748729E-4	0.0
13	0.0	0.0	0.0	2.316031803748729E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACCA	25	0.006037072	18.993511	4
ATGTCGA	35	0.0021633422	16.289589	17
CGTCTTA	95	1.8189894E-11	16.003807	15
CGACCTG	75	5.8113073E-8	15.200092	10
CTAGGAC	70	4.43737E-7	14.921744	3
CGTTTGG	45	6.7361625E-4	14.781295	15
AATCGTA	65	3.3582874E-6	14.615474	13
TGTCCAC	185	0.0	14.378466	10
TAGGACC	445	0.0	14.298487	4
GGTTCGT	40	0.00525452	14.256697	18
TATGTCG	40	0.0052626124	14.25339	16
AGGGTAA	40	0.0052707153	14.250087	10
ACGAAAT	80	1.2809687E-7	14.250087	12
AATTCCG	40	0.0052828887	14.245133	5
CGAGACT	40	0.0052828887	14.245133	4
TAAGAGG	40	0.0052828887	14.245133	4
CTGTAGA	170	0.0	13.969055	1
TTAGAAA	150	0.0	13.926961	3
CTCGTCT	110	1.8371793E-10	13.818266	13
TCTCGTC	110	1.8371793E-10	13.818266	12
>>END_MODULE