##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064069_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431773
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.829750818138233	32.0	32.0	32.0	32.0	32.0
2	31.435066574334197	32.0	32.0	32.0	32.0	32.0
3	31.508343504573006	32.0	32.0	32.0	32.0	32.0
4	31.596329552797418	32.0	32.0	32.0	32.0	32.0
5	31.508938724746567	32.0	32.0	32.0	32.0	32.0
6	35.05700912284927	36.0	36.0	36.0	36.0	36.0
7	35.109664105907505	36.0	36.0	36.0	36.0	36.0
8	35.09140914323036	36.0	36.0	36.0	36.0	36.0
9	35.166358248431465	36.0	36.0	36.0	36.0	36.0
10	35.04431032046932	36.0	36.0	36.0	36.0	36.0
11	35.18613252797187	36.0	36.0	36.0	36.0	36.0
12	35.10595845502151	36.0	36.0	36.0	36.0	36.0
13	35.1516722907639	36.0	36.0	36.0	36.0	36.0
14	35.08842609426713	36.0	36.0	36.0	36.0	36.0
15	35.06707459706837	36.0	36.0	36.0	36.0	36.0
16	35.06892973854317	36.0	36.0	36.0	36.0	36.0
17	35.03409662021479	36.0	36.0	36.0	36.0	36.0
18	35.032459185729536	36.0	36.0	36.0	36.0	36.0
19	34.989765455459235	36.0	36.0	36.0	36.0	36.0
20	34.96753386617505	36.0	36.0	36.0	36.0	36.0
21	34.94743071011851	36.0	36.0	36.0	36.0	36.0
22	34.95568504746707	36.0	36.0	36.0	36.0	36.0
23	34.9489060223775	36.0	36.0	36.0	36.0	36.0
24	34.91911953734949	36.0	36.0	36.0	36.0	36.0
25	34.895577074064384	36.0	36.0	36.0	36.0	36.0
26	34.85904862045565	36.0	36.0	36.0	32.0	36.0
27	34.82449574197553	36.0	36.0	36.0	32.0	36.0
28	34.78309667348352	36.0	36.0	36.0	32.0	36.0
29	34.741213554344526	36.0	36.0	36.0	32.0	36.0
30	34.66766101632108	36.0	36.0	36.0	32.0	36.0
31	34.64670092849715	36.0	36.0	36.0	32.0	36.0
32	34.566869628253734	36.0	36.0	36.0	32.0	36.0
33	34.522459718416854	36.0	36.0	36.0	32.0	36.0
34	34.43704909755821	36.0	36.0	36.0	32.0	36.0
35	34.34675164959365	36.0	36.0	36.0	32.0	36.0
36	34.26404383784998	36.0	36.0	36.0	32.0	36.0
37	34.21257929513888	36.0	36.0	36.0	32.0	36.0
38	34.118342277076145	36.0	36.0	36.0	32.0	36.0
39	33.96151449951711	36.0	36.0	36.0	32.0	36.0
40	33.93876875117249	36.0	36.0	36.0	32.0	36.0
41	33.87675699962712	36.0	36.0	36.0	32.0	36.0
42	33.78449555669298	36.0	36.0	36.0	32.0	36.0
43	33.71566540751738	36.0	36.0	36.0	27.0	36.0
44	33.53524653000535	36.0	36.0	36.0	21.0	36.0
45	33.371892174823344	36.0	36.0	36.0	21.0	36.0
46	33.15219571395154	36.0	36.0	36.0	14.0	36.0
47	33.01941992667443	36.0	36.0	36.0	14.0	36.0
48	32.83490398890157	36.0	36.0	36.0	14.0	36.0
49	32.69336665331088	36.0	36.0	36.0	14.0	36.0
50	32.2125908752979	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	11.0
22	60.0
23	148.0
24	480.0
25	1191.0
26	2428.0
27	4531.0
28	7553.0
29	10906.0
30	14843.0
31	19476.0
32	26900.0
33	39634.0
34	72984.0
35	230622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00252934690966	20.33020484930466	13.093307885447453	22.573957918338227
2	12.83461101975589	22.582856613474767	43.87636936331843	20.70616300345091
3	18.59925423257753	29.837876647289065	29.933992634967694	21.628876485165712
4	9.545988285511136	17.74149842625639	39.38666845773126	33.32584483050121
5	9.944345755755919	42.456568613600204	33.711232522645	13.887853107998879
6	27.214994916310193	40.348053259467356	19.38657581645911	13.05037600776334
7	25.699383703937023	34.23836136117821	21.783205526978296	18.27904940790647
8	26.124607143105287	36.38671246233554	19.832180335500368	17.65650005905881
9	28.631183022324542	13.582557258891947	21.203797418515077	36.58246230026843
10	16.66631925867767	28.326026185298158	32.57899478656412	22.428659769460058
11	32.71001197388442	22.72444085202178	26.984549751837193	17.5809974222566
12	26.19779374810375	26.75781023825019	31.203896491906626	15.840499521739432
13	29.798365804174427	22.397700638299845	28.48007744828289	19.32385610924284
14	21.07334177912931	24.709974917375565	29.36149319202451	24.85519011147061
15	21.623424450034047	34.057819962293344	26.48684204431227	17.83191354336034
16	18.974917201287724	28.1598999467309	32.49438358385252	20.370799268128863
17	17.794072348201485	29.191264854448985	30.453733790672416	22.560929006677117
18	18.103851587650833	26.237580193158394	37.336313314959355	18.322254904231418
19	22.971614648471878	24.720917521284385	31.642857804582047	20.66461002566169
20	23.383869264333953	26.306244962619157	32.35503923366962	17.954846539377264
21	24.081635489018534	23.909554326000006	29.911550745414832	22.097259439566624
22	23.0952910332305	28.22855581186367	30.398914241775753	18.277238913130077
23	20.37478373852685	28.466842223503775	32.482878576275745	18.675495461693636
24	21.95168272070148	27.382339249277976	32.677044081237504	17.988933948783036
25	20.578644086639123	28.311037408978894	33.304228196624116	17.80609030775787
26	17.41547269973179	29.862375334102293	34.94258304497468	17.77956892119123
27	18.898711983119824	29.360809173848697	33.01223627616972	18.728242566861763
28	17.49920671868739	29.775727213139053	32.305657659160026	20.419408409013528
29	19.05689437541255	27.240975993885563	31.564161059835786	22.137968570866096
30	17.64035575319622	28.674495089864738	33.06906614785992	20.616083009079116
31	20.92014351329629	27.470659220775612	31.38137338240642	20.22782388352168
32	18.155098977911493	30.51627860655035	31.82556170862255	19.5030607069156
33	18.27271611314803	30.088499674582458	32.9262332861613	18.712550926108214
34	19.214984181099773	29.626275587013218	33.40937831490497	17.74936191698204
35	18.865110246433204	30.80229757272559	31.98929961089494	18.343292569946264
36	17.440971691426824	30.447813740613604	32.64611735712532	19.465097210834248
37	19.231285899573837	30.17718176764869	29.475403001667594	21.116129331109875
38	18.93440002964761	32.46575469381894	29.93551644747509	18.664328829058363
39	20.369276663354665	29.9951593814199	29.762856011283965	19.872707943941467
40	21.402052495906357	30.425437793995375	31.004685397448164	17.167824312650108
41	20.700668660062675	29.173219319110892	32.105410657334296	18.020701363492133
42	19.490791350910676	33.448750254766445	30.257453076652276	16.8030053176706
43	17.317905259086498	32.68244215051174	30.816389395206212	19.183263195195558
44	17.20333232199476	32.612889703106504	30.077517943539515	20.106260031359216
45	18.044518969546356	32.61219489034697	29.600644786240853	19.74264135386582
46	19.579360355373566	30.198415363699738	28.931076890296016	21.291147390630684
47	21.98135100957463	30.748831543011722	27.718949616227306	19.550867831186338
48	19.11994608228011	32.02823732134544	27.196023799763296	21.655792796611152
49	20.517030939868867	31.111023616576304	27.731470008546154	20.640475435008675
50	18.18360786348224	33.8654092012377	27.043690129884567	20.907292805395493
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	96.0
1	79.0
2	62.0
3	172.5
4	283.0
5	349.5
6	416.0
7	391.0
8	366.0
9	524.0
10	682.0
11	1045.0
12	1408.0
13	2506.0
14	3604.0
15	5098.0
16	6592.0
17	7514.0
18	8436.0
19	8522.5
20	8609.0
21	8768.0
22	8927.0
23	8994.5
24	9062.0
25	8374.5
26	7687.0
27	9561.0
28	11435.0
29	13755.5
30	16076.0
31	18225.5
32	20375.0
33	24973.5
34	29572.0
35	33630.0
36	37688.0
37	38314.0
38	38940.0
39	35070.5
40	31201.0
41	28867.5
42	26534.0
43	22886.5
44	19239.0
45	19000.0
46	18761.0
47	18703.5
48	18646.0
49	18110.0
50	17574.0
51	16234.5
52	14895.0
53	13739.5
54	12584.0
55	11934.0
56	11284.0
57	11135.0
58	10986.0
59	9985.0
60	8984.0
61	8061.0
62	7138.0
63	6518.5
64	5899.0
65	5593.5
66	5288.0
67	5033.0
68	4778.0
69	4086.5
70	3395.0
71	2450.5
72	1506.0
73	1250.5
74	995.0
75	804.5
76	614.0
77	546.0
78	478.0
79	361.5
80	245.0
81	205.0
82	165.0
83	140.0
84	115.0
85	96.5
86	78.0
87	55.5
88	33.0
89	25.0
90	17.0
91	11.5
92	6.0
93	4.5
94	3.0
95	7.0
96	11.0
97	7.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00949573039536979
2	6.948095411246188E-4
3	6.948095411246188E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0013896190822492375
10	9.264127214994916E-4
11	0.0
12	0.0
13	2.316031803748729E-4
14	0.0
15	0.004400460427122585
16	6.948095411246188E-4
17	0.0
18	6.948095411246188E-4
19	2.316031803748729E-4
20	2.316031803748729E-4
21	0.0
22	2.316031803748729E-4
23	9.264127214994916E-4
24	4.632063607497458E-4
25	0.0011580159018743646
26	0.004400460427122585
27	0.00555847632899695
28	0.005095269968247204
29	0.0018528254429989832
30	0.003010841344873348
31	0.008337714493495425
32	0.002316031803748729
33	0.004632063607497458
34	0.002547634984123602
35	0.003010841344873348
36	0.007179698591621059
37	0.003010841344873348
38	0.008106111313120553
39	0.002316031803748729
40	0.0013896190822492375
41	0.003242444525248221
42	0.0011580159018743646
43	0.0013896190822492375
44	4.632063607497458E-4
45	4.632063607497458E-4
46	4.632063607497458E-4
47	0.0016212222626241105
48	9.264127214994916E-4
49	0.0
50	0.0011580159018743646
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	431773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.00428428406666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.45120441426491	30.1181406680961
2	13.11159899141196	10.752634649250751
3	4.981543008287108	6.127938170553276
4	2.4668863073580622	4.0461162977352565
5	1.3279502535367278	2.7225824855559186
6	0.87407995932096	2.1504613883401236
7	0.6004289653406483	1.7234111991051164
8	0.4518915647202898	1.4823592148289977
9	0.3409451693459487	1.2582191384226475
>10	1.9890187186097794	15.811316873312292
>50	0.22488473554349175	6.421883574851245
>100	0.16767224614829607	13.010539155154714
>500	0.009629824947706193	2.7977281416045727
>1k	0.002265841164166163	1.5766690431890278
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2354	0.5451938866024508	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1572	0.3640801995493002	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	1536	0.3557424850558048	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1326	0.30710581717708146	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	995	0.23044516447299854	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	948	0.2195598149953795	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	940	0.21770698955238055	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	822	0.19037781426814554	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	796	0.18435613157839884	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	765	0.17717643298677777	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	699	0.16189062308203617	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	696	0.16119581354091156	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	681	0.15772176583528844	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	667	0.15447932131004025	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	656	0.15193168632591664	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	616	0.14266755911092172	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	583	0.13502465415855092	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	576	0.1334034318959268	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	569	0.13178220963330267	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	525	0.12159166969680826	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	511	0.11834922517156006	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	465	0.10769547887431591	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA	457	0.10584265343131691	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	454	0.1051478438901923	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	439	0.1016737961845692	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	438	0.10144219300419434	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	436	0.1009789866434446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.316031803748729E-4	0.0
12	0.0	0.0	0.0	4.632063607497458E-4	0.0
13	0.0	0.0	0.0	4.632063607497458E-4	0.0
14	0.0	0.0	0.0	4.632063607497458E-4	0.0
15	0.0	0.0	0.0	4.632063607497458E-4	0.0
16	0.0	0.0	0.0	4.632063607497458E-4	0.0
17	0.0	0.0	0.0	4.632063607497458E-4	0.0
18	0.0	0.0	0.0	6.948095411246188E-4	0.0
19	0.0	0.0	0.0	9.264127214994916E-4	0.0
20	0.0	0.0	0.0	9.264127214994916E-4	0.0
21	0.0	0.0	0.0	0.0013896190822492375	0.0
22	0.0	0.0	0.0	0.004863666787872331	0.0
23	0.0	0.0	0.0	0.008337714493495425	0.0
24	0.0	0.0	0.0	0.016443825806615978	0.0
25	0.0	0.0	0.0	0.020844286233738563	0.0
26	0.0	0.0	0.0	0.025939556201985764	0.0
27	0.0	0.0	0.0	0.03172963571135759	0.0
28	0.0	0.0	0.0	0.041225366106727376	0.0
29	0.0	0.0	0.0	0.05859560463484285	0.0
30	0.0	0.0	0.0	0.10236860572569383	0.0
31	2.316031803748729E-4	0.0	0.0	0.17393398846152955	0.0
32	2.316031803748729E-4	0.0	0.0	0.23994089486836834	0.0
33	2.316031803748729E-4	0.0	0.0	0.29737848360133684	0.0
34	2.316031803748729E-4	0.0	0.0	0.36639623135304894	0.0
35	2.316031803748729E-4	0.0	0.0	0.4430568840571319	0.0
36	2.316031803748729E-4	0.0	0.0	0.5570056488015693	0.0
37	2.316031803748729E-4	0.0	0.0	0.6994416047321161	0.0
38	2.316031803748729E-4	0.0	0.0	0.872912386832896	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAGA	20	7.8547816E-4	43.995716	19
CACGAAA	30	1.2987226E-4	36.67159	37
CAGTCGC	25	0.0023495748	35.204727	31
GTCCAAA	25	0.0023495748	35.204727	1
TCGCGAG	30	0.0057437085	29.330477	18
ATTTAGG	40	7.019251E-4	27.50369	1
GCGACTA	40	7.0287636E-4	27.497322	15
GGCGACT	40	7.0287636E-4	27.497322	14
TGGCTTG	40	7.0287636E-4	27.497322	18
GTTACTC	50	8.321634E-5	26.403545	35
AGGCACG	50	8.334761E-5	26.397429	8
GTACGAC	45	0.0013975528	24.442064	3
TAGGACC	540	0.0	24.442062	4
CAGTGAT	430	0.0	24.04974	37
GTCCTAT	65	1.8080107E-5	23.695488	1
AGGACCT	755	0.0	22.726263	5
TGTAGGA	1170	0.0	22.37389	2
GGACCTG	795	0.0	22.136208	6
CCCCTCG	50	0.0025757672	22.002954	25
GTTCTAA	60	2.8660323E-4	22.002954	1
>>END_MODULE