FastQCFastQC Report
Thu 2 Feb 2017
SRR4064068_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064068_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2778336
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51630.18583065547147645No Hit
TATCAACGCAGAGTACTTTTTTTTT32970.11866815244808403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATA350.002168290516.2885113
CGTCTTA801.2858254E-714.25347215
CGGTCCA4200.013.79899210
GGTATCA11550.013.3197621
GTATCAA34800.013.2896391
CGTTATT2550.013.0327662
CGCCGGT4400.012.5230717
GCCGGTC4650.012.4631588
AAGACGG6050.011.9305595
GCGTTAT2800.011.8706231
GCGTAAC2350.011.72478211
TCTTATA1900.011.494312
CGCGTAA2400.011.480309510
CAAGACG6400.011.4262964
AACCGCG2550.011.1767787
CGTCGTA4150.010.98904610
CCGTCGT4150.010.9888489
CCGGTCC5550.010.9558489
GGGTCGC2800.010.8552916
CGCAAGA6400.010.8305782
Produced by FastQC (version 0.11.5)