##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064068_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2778336
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.242156816166222	32.0	32.0	32.0	32.0	32.0
2	30.770775025050966	32.0	32.0	32.0	32.0	32.0
3	30.79402923188556	32.0	32.0	32.0	32.0	32.0
4	30.833670585559126	32.0	32.0	32.0	32.0	32.0
5	30.70018853011299	32.0	32.0	32.0	32.0	32.0
6	34.37941091358281	36.0	36.0	36.0	32.0	36.0
7	34.31854858447647	36.0	36.0	36.0	32.0	36.0
8	34.28164376086981	36.0	36.0	36.0	32.0	36.0
9	34.45002584280663	36.0	36.0	36.0	32.0	36.0
10	34.089446704790205	36.0	36.0	36.0	32.0	36.0
11	34.41886402508552	36.0	36.0	36.0	32.0	36.0
12	34.20404155580894	36.0	36.0	36.0	32.0	36.0
13	34.30442322310908	36.0	36.0	36.0	32.0	36.0
14	34.186951110304875	36.0	36.0	36.0	32.0	36.0
15	34.11252454706702	36.0	36.0	36.0	32.0	36.0
16	34.11735477638414	36.0	36.0	36.0	32.0	36.0
17	34.031325224882806	36.0	36.0	36.0	32.0	36.0
18	34.029790133374796	36.0	36.0	36.0	32.0	36.0
19	34.054852256890456	36.0	36.0	36.0	32.0	36.0
20	34.039621917579446	36.0	36.0	36.0	32.0	36.0
21	34.02785120302224	36.0	36.0	36.0	32.0	36.0
22	34.00318248044873	36.0	36.0	36.0	32.0	36.0
23	33.93284577531299	36.0	36.0	36.0	32.0	36.0
24	33.92154008730405	36.0	36.0	36.0	32.0	36.0
25	33.49952597526001	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	12.0
4	22.0
5	46.0
6	103.0
7	52.0
8	148.0
9	175.0
10	242.0
11	71.0
12	186.0
13	169.0
14	230.0
15	444.0
16	707.0
17	1031.0
18	1399.0
19	1866.0
20	2898.0
21	4495.0
22	7023.0
23	10924.0
24	16232.0
25	23697.0
26	33405.0
27	43547.0
28	59120.0
29	78737.0
30	103640.0
31	140313.0
32	196605.0
33	275487.0
34	577782.0
35	1197527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73438921733732	16.935408661748763	11.202798215036085	26.127403905877827
2	16.97500068390688	19.311370563784035	37.101984193832166	26.611644558476915
3	18.744866439236276	23.224426858861904	28.493718789210327	29.536987912691494
4	12.519288450110269	15.274580783286545	35.84683989767538	36.35929086892781
5	14.780609190739355	36.16855388201704	33.62258596677776	15.428250960465848
6	34.56162126087028	35.488335794348856	16.656995391625443	13.293047553155416
7	30.803243560831888	30.232661119607386	20.473717710556404	18.490377609004323
8	28.42270204137771	32.60558747802342	19.05649514706078	19.915215333538082
9	27.31075097516147	14.080352152845396	18.32813823381981	40.28075863817332
10	15.670226504816117	26.51356745625591	31.428794713759196	26.38741132516878
11	37.265701526937484	20.992221261884183	22.477924229328725	19.264152981849612
12	24.872484903840544	23.373853876811882	28.729019705863497	23.024641513484077
13	29.3115464031144	19.484629538988816	25.229648406084294	25.974175651812498
14	23.831741702738583	19.37096194421693	25.052820236868058	31.74447611617643
15	25.43252551539495	27.01882155412066	21.983125751578246	25.565527178906144
16	26.102968586740065	25.729720696186735	23.270959671723386	24.896351045349814
17	24.293507562751007	25.799863068412847	24.878870289195046	25.0277590796411
18	24.982115571231898	24.94494189451753	25.71924940871521	24.35369312553536
19	25.74752290511325	24.665734469259817	25.20844769680964	24.37829492881729
20	26.023087527209633	24.200442990622562	24.592247267870285	25.184222214297524
21	27.01995724348341	24.11341463194996	24.274931245576216	24.591696878990412
22	26.199001204548466	24.020151692419415	24.74842803543332	25.0324190675988
23	24.68010638324698	24.04635300923883	25.162346843534998	26.11119376397919
24	24.76211437241276	24.866108286229288	25.01804674430969	25.35373059704826
25	25.013445048205856	24.332025150254943	25.08282257759495	25.57170722394425
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	109.0
2	348.5
3	588.0
4	588.0
5	588.0
6	1567.5
7	2547.0
8	2547.0
9	2547.0
10	3225.0
11	3903.0
12	3903.0
13	3903.0
14	5548.0
15	7193.0
16	7193.0
17	7193.0
18	12363.0
19	17533.0
20	17533.0
21	17533.0
22	27776.5
23	38020.0
24	38020.0
25	38020.0
26	55523.0
27	73026.0
28	73026.0
29	73026.0
30	91429.5
31	109833.0
32	109833.0
33	109833.0
34	138288.0
35	166743.0
36	166743.0
37	166743.0
38	195270.0
39	223797.0
40	223797.0
41	223797.0
42	257096.0
43	290395.0
44	290395.0
45	290395.0
46	323435.0
47	356475.0
48	356475.0
49	356475.0
50	368314.0
51	380153.0
52	380153.0
53	380153.0
54	356829.5
55	333506.0
56	333506.0
57	333506.0
58	307472.0
59	281438.0
60	281438.0
61	281438.0
62	247126.5
63	212815.0
64	212815.0
65	212815.0
66	175013.5
67	137212.0
68	137212.0
69	137212.0
70	102837.0
71	68462.0
72	68462.0
73	68462.0
74	52494.5
75	36527.0
76	36527.0
77	36527.0
78	28857.0
79	21187.0
80	21187.0
81	21187.0
82	14764.0
83	8341.0
84	8341.0
85	8341.0
86	6372.0
87	4403.0
88	4403.0
89	4403.0
90	3102.0
91	1801.0
92	1801.0
93	1801.0
94	1279.5
95	758.0
96	758.0
97	758.0
98	1164.5
99	1571.0
100	1571.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04092377595798349
2	0.006478698040841713
3	0.0017996383446782533
4	0.004283139260334243
5	0.009250141091646222
6	0.012597468412747776
7	0.023323312947030165
8	0.03408515024820612
9	0.04895016297524849
10	0.05823629683378829
11	0.05528488994851594
12	0.06485896594220425
13	0.06511091531045922
14	0.0697539822397291
15	0.060431855614295746
16	0.06827827879709293
17	0.06359921910092949
18	0.07839224629418472
19	0.07558481047648664
20	0.07936405100031098
21	0.07742044158805847
22	0.07889614503069463
23	0.08404311069647444
24	0.07961600036856593
25	0.07993993527060801
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2778336.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.21653875432245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.96095052702401	28.423316899319985
2	15.469330482182375	12.751845186445829
3	6.065111335410337	7.499486892156616
4	3.0034176200212563	4.951619149240841
5	1.6525014994452227	3.405519604672999
6	1.0444544644101443	2.5829278745691493
7	0.692713366620117	1.9985873124654658
8	0.4957816070504845	1.634752145654125
9	0.36293292284533274	1.346295499170676
>10	1.984424468263921	15.17858706783371
>50	0.15514040776066615	4.412369862140187
>100	0.09422770114464345	7.7882024197278525
>500	0.012179217037850422	3.468356391459984
>1k	0.006746760517363658	4.37167690103707
>5k	8.76202664592683E-5	0.186456794105462
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5163	0.18583065547147645	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3297	0.11866815244808403	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.198553378713013E-5
2	0.0	0.0	0.0	0.0	7.198553378713013E-5
3	0.0	0.0	0.0	0.0	7.198553378713013E-5
4	0.0	0.0	0.0	0.0	7.198553378713013E-5
5	0.0	0.0	0.0	0.0	7.198553378713013E-5
6	0.0	0.0	0.0	0.0	1.4397106757426027E-4
7	0.0	0.0	0.0	0.0	2.159566013613904E-4
8	0.0	0.0	0.0	0.0	2.159566013613904E-4
9	0.0	0.0	0.0	0.0	2.159566013613904E-4
10	0.0	0.0	0.0	0.0	2.159566013613904E-4
11	0.0	0.0	0.0	0.0	2.159566013613904E-4
12	0.0	0.0	0.0	0.0	5.39891503403476E-4
13	0.0	0.0	0.0	0.0	5.39891503403476E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATA	35	0.0021682905	16.28851	13
CGTCTTA	80	1.2858254E-7	14.253472	15
CGGTCCA	420	0.0	13.798992	10
GGTATCA	1155	0.0	13.319762	1
GTATCAA	3480	0.0	13.289639	1
CGTTATT	255	0.0	13.032766	2
CGCCGGT	440	0.0	12.523071	7
GCCGGTC	465	0.0	12.463158	8
AAGACGG	605	0.0	11.930559	5
GCGTTAT	280	0.0	11.870623	1
GCGTAAC	235	0.0	11.724782	11
TCTTATA	190	0.0	11.49431	2
CGCGTAA	240	0.0	11.4803095	10
CAAGACG	640	0.0	11.426296	4
AACCGCG	255	0.0	11.176778	7
CGTCGTA	415	0.0	10.989046	10
CCGTCGT	415	0.0	10.988848	9
CCGGTCC	555	0.0	10.955848	9
GGGTCGC	280	0.0	10.855291	6
CGCAAGA	640	0.0	10.830578	2
>>END_MODULE