FastQCFastQC Report
Thu 2 Feb 2017
SRR4064068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2778336
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33900.029.65961
GGTATCA13250.024.4090161
TCAACGC52700.018.7422624
TATCAAC53600.018.6331022
CAACGCA53550.018.4447675
ATCAACG53650.018.3693833
GTATACG1203.163641E-718.3344361
CGCAATA6550.018.13882336
AACGCAG54850.018.0878186
CTAGCGG6800.017.1480929
TTAGTAC2109.094947E-1216.76053
TCTAGCG7050.016.54000328
TAGCGGC7050.016.22851230
TAAACGC5050.016.11978128
ATACGAA7200.015.88812840
GGCGTTA7550.015.44296942
ACGCAGA64850.015.298647
TACGACG8350.015.2801555
ACGACGG8500.015.2693086
GTAAACG5100.015.0994927
Produced by FastQC (version 0.11.5)