##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064068_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2778336
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.333285103025695	32.0	32.0	32.0	32.0	32.0
2	31.395964706932496	32.0	32.0	32.0	32.0	32.0
3	31.470250538451793	32.0	32.0	32.0	32.0	32.0
4	31.556898085760686	32.0	32.0	32.0	32.0	32.0
5	31.50252597238059	32.0	32.0	32.0	32.0	32.0
6	35.087942207134056	36.0	36.0	36.0	36.0	36.0
7	35.10525940706955	36.0	36.0	36.0	36.0	36.0
8	35.04551105409857	36.0	36.0	36.0	36.0	36.0
9	35.15543728332354	36.0	36.0	36.0	36.0	36.0
10	35.011319365260356	36.0	36.0	36.0	36.0	36.0
11	35.164747172408234	36.0	36.0	36.0	36.0	36.0
12	35.05888128721652	36.0	36.0	36.0	36.0	36.0
13	35.105361266599864	36.0	36.0	36.0	36.0	36.0
14	35.04747265989427	36.0	36.0	36.0	36.0	36.0
15	35.019959788880826	36.0	36.0	36.0	36.0	36.0
16	35.035086469023184	36.0	36.0	36.0	36.0	36.0
17	34.994683508402154	36.0	36.0	36.0	36.0	36.0
18	34.995486147103875	36.0	36.0	36.0	36.0	36.0
19	34.995912301463896	36.0	36.0	36.0	36.0	36.0
20	34.98830666989162	36.0	36.0	36.0	36.0	36.0
21	34.9846015744676	36.0	36.0	36.0	36.0	36.0
22	34.971432180988906	36.0	36.0	36.0	36.0	36.0
23	34.909663194084516	36.0	36.0	36.0	32.0	36.0
24	34.883249182244334	36.0	36.0	36.0	32.0	36.0
25	34.853555149557145	36.0	36.0	36.0	32.0	36.0
26	34.802143801181714	36.0	36.0	36.0	32.0	36.0
27	34.792197919906016	36.0	36.0	36.0	32.0	36.0
28	34.766216541123896	36.0	36.0	36.0	32.0	36.0
29	34.74537025039448	36.0	36.0	36.0	32.0	36.0
30	34.73019641972749	36.0	36.0	36.0	32.0	36.0
31	34.71840662900384	36.0	36.0	36.0	32.0	36.0
32	34.68317222970872	36.0	36.0	36.0	32.0	36.0
33	34.66412917660067	36.0	36.0	36.0	32.0	36.0
34	34.65224616461076	36.0	36.0	36.0	32.0	36.0
35	34.61853965827027	36.0	36.0	36.0	32.0	36.0
36	34.59553308167191	36.0	36.0	36.0	32.0	36.0
37	34.588274420372485	36.0	36.0	36.0	32.0	36.0
38	34.548414950531544	36.0	36.0	36.0	32.0	36.0
39	34.55214308132638	36.0	36.0	36.0	32.0	36.0
40	34.528472438178824	36.0	36.0	36.0	32.0	36.0
41	34.51698858597376	36.0	36.0	36.0	32.0	36.0
42	34.45706998721537	36.0	36.0	36.0	32.0	36.0
43	34.43917906257558	36.0	36.0	36.0	32.0	36.0
44	34.381438026214255	36.0	36.0	36.0	32.0	36.0
45	34.36263396507838	36.0	36.0	36.0	32.0	36.0
46	34.34428161316702	36.0	36.0	36.0	32.0	36.0
47	34.317296396116234	36.0	36.0	36.0	32.0	36.0
48	34.28059385185953	36.0	36.0	36.0	32.0	36.0
49	34.26580550372597	36.0	36.0	36.0	32.0	36.0
50	33.65537789525817	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	6.0
19	8.0
20	22.0
21	83.0
22	328.0
23	941.0
24	2358.0
25	5261.0
26	10707.0
27	19201.0
28	31351.0
29	48365.0
30	69619.0
31	98775.0
32	142502.0
33	230240.0
34	482305.0
35	1636263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69067601224015	17.863258354448107	11.787099753749994	26.65896587956175
2	16.171811618667668	19.996062371460184	36.657270107640514	27.174855902231638
3	17.95398952749931	23.705910469765225	28.860060957613292	29.48003904512217
4	12.165951130460822	15.907075314144869	35.87838188037732	36.048591675016986
5	14.344773274362785	36.61015082409039	33.41161760132684	15.633458300219989
6	34.63879380779101	35.81809936184687	16.384626736204844	13.158480094157282
7	30.688379910989855	30.39835009097175	20.480719567654727	18.432550430383664
8	28.385803588910775	32.796969121085425	18.918554127362565	19.898673162641238
9	27.278342211054735	13.890889510611334	18.21529185261651	40.615476425717425
10	15.442619634734916	26.68689718321671	31.533294868717	26.33718831333137
11	37.41283898449971	20.885062456471232	22.453951737281503	19.248146821747557
12	24.777699433437245	23.367645275062422	28.73751994451349	23.117135346986846
13	29.396736591367016	19.40585000586685	25.18556164476256	26.011851758003573
14	23.7946896157044	19.247569583166865	25.08524905248837	31.872491748640368
15	25.450299419733664	27.07145291909494	21.93670234075431	25.541545320417093
16	26.101522758594875	25.738352734195175	23.265413220180736	24.894711287029214
17	24.26755592828079	25.846955100806777	24.82227665130375	25.063212319608684
18	25.03276247502547	24.94339227399471	25.660477459309206	24.363367791670612
19	25.71528425647582	24.723539557490525	25.193929028022527	24.367247158011125
20	26.079260271063575	24.103062467718743	24.619742541792416	25.197934719425263
21	26.972259654699794	24.134517927277336	24.292310217338724	24.60091220068415
22	26.139225881409505	24.054199387114725	24.783773297234962	25.02280143424081
23	24.726911618669316	23.924950140606907	25.202667949939407	26.145470290784374
24	24.87886590702459	24.774414521550025	24.992207537920315	25.354512033505067
25	25.03315882582663	24.24328590016539	25.204198978150266	25.51935629585771
26	25.026384217558572	24.939996602087124	25.22165622336382	24.811962956990484
27	25.497234864464446	24.588685189211294	24.64498136179146	25.269098584532806
28	25.028417414565986	24.296043590406253	25.484391838532932	25.191147156494825
29	24.87772940224543	24.58538857358408	25.301735184273344	25.235146839897144
30	24.85892137390677	24.699359810066667	25.454046773530564	24.987672042495998
31	25.340697535459178	24.443617347533802	24.571293433986387	25.64439168302063
32	25.230711862039723	24.563457055145165	24.416745943218192	25.789085139596917
33	24.79134984153494	24.401207249978853	24.94608948551498	25.861353422971224
34	25.479102965339877	24.38032369122456	25.19777631003522	24.94279703340034
35	25.9931712949632	24.123879879291877	25.202463977609103	24.68048484813582
36	24.915284863869058	24.708635364600845	24.85423669268653	25.52184307884357
37	25.89279800966946	24.426901713031885	24.716402373883824	24.963897903414832
38	25.11733700873508	24.228315015717524	25.103514656912314	25.550833318635085
39	25.634634337023716	24.407227089057933	24.484542947375544	25.473595626542807
40	25.784022917883703	24.480224542853513	24.91542389554352	24.820328643719268
41	24.712434540321794	24.95021123107928	25.379584247132264	24.95776998146666
42	25.942153606050343	24.752068629289464	24.95420742766501	24.35157033699518
43	25.419357764615434	23.81884435639687	25.143937147085836	25.617860731901853
44	24.92116550869978	24.610722642604856	24.8449315387996	25.623180309895766
45	25.145457706315987	24.67671766069815	24.86658203454704	25.311242598438827
46	24.982957240851082	24.544596599510637	24.892506440975186	25.5799397186631
47	25.05668073171864	24.465127897430104	25.27667287794143	25.201518492909823
48	25.84578073355167	24.924350976913505	24.287448650987244	24.942419638547584
49	25.069691320987513	24.972042487500552	24.601531211453818	25.356734980058114
50	25.159800669832865	25.004922065791913	24.61133676828138	25.223940496093846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	37.5
2	55.0
3	178.5
4	302.0
5	354.5
6	407.0
7	383.0
8	359.0
9	473.5
10	588.0
11	908.0
12	1228.0
13	2278.0
14	3328.0
15	4372.0
16	5416.0
17	5235.5
18	5055.0
19	5139.5
20	5224.0
21	6281.0
22	7338.0
23	8846.5
24	10355.0
25	13172.5
26	15990.0
27	19643.0
28	23296.0
29	27322.0
30	31348.0
31	35475.0
32	39602.0
33	45775.0
34	51948.0
35	60013.0
36	68078.0
37	81575.5
38	95073.0
39	105845.5
40	116618.0
41	128655.5
42	140693.0
43	146514.5
44	152336.0
45	167365.0
46	182394.0
47	194229.5
48	206065.0
49	213275.0
50	220485.0
51	215662.5
52	210840.0
53	209009.5
54	207179.0
55	206981.5
56	206784.0
57	201068.5
58	195353.0
59	178534.5
60	161716.0
61	142315.0
62	122914.0
63	107753.5
64	92593.0
65	78117.5
66	63642.0
67	54394.0
68	45146.0
69	40139.0
70	35132.0
71	26984.5
72	18837.0
73	16134.0
74	13431.0
75	10448.5
76	7466.0
77	6334.0
78	5202.0
79	4255.0
80	3308.0
81	2747.5
82	2187.0
83	1796.5
84	1406.0
85	1090.0
86	774.0
87	556.0
88	338.0
89	264.5
90	191.0
91	145.5
92	100.0
93	88.0
94	76.0
95	69.0
96	62.0
97	46.0
98	30.0
99	29.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009538083226794742
2	5.03898736509911E-4
3	4.319132027227808E-4
4	0.0
5	0.0
6	2.159566013613904E-4
7	3.599276689356507E-5
8	0.0
9	0.0011877613074876472
10	8.998191723391266E-4
11	3.599276689356507E-5
12	1.079783006806952E-4
13	5.03898736509911E-4
14	2.159566013613904E-4
15	0.004643066929269894
16	8.278336385519967E-4
17	3.599276689356507E-5
18	1.7996383446782535E-4
19	0.0
20	7.558481047648664E-4
21	0.0
22	7.198553378713013E-5
23	8.278336385519967E-4
24	3.599276689356507E-4
25	0.0014757034426361678
26	0.005758842702970411
27	0.006190755905693192
28	0.003959204358292157
29	0.002591479216336685
30	0.0035272911555693765
31	0.007630466581435794
32	0.0031313707197401608
33	0.004355124794121373
34	0.00255548644944312
35	0.0023035370811881643
36	0.007270538912500144
37	0.003743247756930767
38	0.008170358084839272
39	0.0043911175610149384
40	0.00248350091565599
41	0.003563283922462942
42	0.0016916600439975584
43	0.0014397106757426028
44	0.0016916600439975584
45	0.0013317323750619077
46	0.001079783006806952
47	0.0019796021791460786
48	0.0016196745102104281
49	4.679059696163459E-4
50	0.0011157757737005171
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2778336.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.50103301189747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.67783408230397	30.310577606118194
2	15.35320402908793	13.357604706155026
3	5.892666443171124	7.690112324174625
4	2.9469422064802795	5.127801208330104
5	1.5789009296211545	3.434191073098272
6	1.0091251749494816	2.6338792529168527
7	0.6369461581760789	1.9395471097533212
8	0.4531455972005529	1.5769841266413798
9	0.3482977811454224	1.3636181948019899
>10	1.8707072329452172	14.846670890734142
>50	0.1359488814632974	4.089898952990407
>100	0.08064484958514441	6.972394030589875
>500	0.010563045221091312	3.2049456141875576
>1k	0.005073588649489871	3.4517749095082366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.599276689356507E-5
2	0.0	0.0	0.0	0.0	3.599276689356507E-5
3	0.0	0.0	0.0	0.0	3.599276689356507E-5
4	0.0	0.0	0.0	0.0	3.599276689356507E-5
5	0.0	0.0	0.0	0.0	3.599276689356507E-5
6	0.0	0.0	0.0	0.0	7.198553378713013E-5
7	0.0	0.0	0.0	0.0	1.079783006806952E-4
8	0.0	0.0	0.0	0.0	1.079783006806952E-4
9	0.0	0.0	0.0	0.0	1.079783006806952E-4
10	0.0	0.0	0.0	0.0	1.079783006806952E-4
11	0.0	0.0	0.0	0.0	1.079783006806952E-4
12	0.0	0.0	0.0	0.0	1.7996383446782535E-4
13	0.0	0.0	0.0	0.0	1.7996383446782535E-4
14	0.0	0.0	0.0	0.0	1.7996383446782535E-4
15	0.0	0.0	0.0	0.0	3.599276689356507E-4
16	0.0	0.0	0.0	0.0	3.599276689356507E-4
17	0.0	0.0	0.0	0.0	3.599276689356507E-4
18	0.0	0.0	0.0	0.0	4.319132027227808E-4
19	0.0	0.0	0.0	0.0	4.319132027227808E-4
20	0.0	0.0	0.0	7.198553378713013E-5	5.758842702970411E-4
21	0.0	0.0	0.0	1.4397106757426027E-4	6.838625709777363E-4
22	0.0	0.0	0.0	2.8794213514852054E-4	7.198553378713014E-4
23	0.0	0.0	0.0	4.679059696163459E-4	7.198553378713014E-4
24	0.0	0.0	0.0	6.478698040841712E-4	7.198553378713014E-4
25	0.0	0.0	0.0	6.838625709777363E-4	7.198553378713014E-4
26	0.0	0.0	0.0	9.358119392326918E-4	8.278336385519966E-4
27	0.0	0.0	0.0	0.0011877613074876472	8.638264054455616E-4
28	0.0	0.0	0.0	0.002519493682549555	8.638264054455616E-4
29	0.0	0.0	0.0	0.00511097289888624	8.638264054455616E-4
30	0.0	0.0	0.0	0.010617866233601695	8.638264054455616E-4
31	0.0	0.0	0.0	0.0247270308558792	8.638264054455616E-4
32	0.0	0.0	0.0	0.040563848289047834	8.998191723391267E-4
33	0.0	0.0	0.0	0.05661662232357785	8.998191723391267E-4
34	0.0	0.0	0.0	0.07497293343929604	0.001043790239913387
35	0.0	0.0	0.0	0.09865617405526185	0.001043790239913387
36	0.0	0.0	0.0	0.13227341833385162	0.001043790239913387
37	0.0	0.0	0.0	0.1799278417009318	0.001043790239913387
38	0.0	0.0	0.0	0.24968182394066088	0.001079783006806952
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3390	0.0	29.6596	1
GGTATCA	1325	0.0	24.409016	1
TCAACGC	5270	0.0	18.742262	4
TATCAAC	5360	0.0	18.633102	2
CAACGCA	5355	0.0	18.444767	5
ATCAACG	5365	0.0	18.369383	3
GTATACG	120	3.163641E-7	18.334436	1
CGCAATA	655	0.0	18.138823	36
AACGCAG	5485	0.0	18.087818	6
CTAGCGG	680	0.0	17.14809	29
TTAGTAC	210	9.094947E-12	16.7605	3
TCTAGCG	705	0.0	16.540003	28
TAGCGGC	705	0.0	16.228512	30
TAAACGC	505	0.0	16.119781	28
ATACGAA	720	0.0	15.888128	40
GGCGTTA	755	0.0	15.442969	42
ACGCAGA	6485	0.0	15.29864	7
TACGACG	835	0.0	15.280155	5
ACGACGG	850	0.0	15.269308	6
GTAAACG	510	0.0	15.09949	27
>>END_MODULE