FastQCFastQC Report
Thu 2 Feb 2017
SRR4064067_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064067_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339632
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43600.3254625150787679No Hit
TATCAACGCAGAGTACTTTTTTTTT32160.2400659285535132No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31990.23879692333416938No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20320.15168344739450834No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17320.12928923764138212No Hit
GTACATGGGGTGGTATCAACGCAAA16520.12331744837388177No Hit
GGTATCAACGCAGAGTACTTTTTTT15950.1190625485207878No Hit
GTCCTAAAGTGTGTATTTCTCATTT15570.11622594861872514No Hit
GTCCTACAGTGGACATTTCTAAATT14040.10480490164463076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA651.9103936E-716.07862916
CGTCTTA508.7046195E-515.20161415
ACGAACG704.429312E-714.93015615
AATCGTA456.761765E-414.77769113
GCGTTAT801.2868622E-714.2509791
CGCGGTC852.695815E-713.41268710
TAGACTG802.0023017E-613.0604725
CGAGGTT802.004821E-613.0590094
AACGAAC957.3805495E-813.00040814
GTCTAGA1403.6379788E-1212.8937441
CGTAAAT604.0877078E-412.66801116
GGCTTAG604.088996E-412.6675381
AAGACGG1352.7284841E-1112.66470055
ATTTAGA2850.012.3341821
CGATAAC853.9391307E-612.29496310
AACCGCG853.942423E-612.2940457
ACACCGT853.9457154E-612.2931276
CGAACGT550.00307421212.0876794
ACTGTTC5050.012.0399838
AGGCCCG1501.4551915E-1112.03416210