##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064067_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1339632
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226224813978764	32.0	32.0	32.0	32.0	32.0
2	30.87922354795944	32.0	32.0	32.0	32.0	32.0
3	30.911780996572194	32.0	32.0	32.0	32.0	32.0
4	30.921520984867485	32.0	32.0	32.0	32.0	32.0
5	30.86131713784084	32.0	32.0	32.0	32.0	32.0
6	34.51560503182964	36.0	36.0	36.0	32.0	36.0
7	34.439227340045626	36.0	36.0	36.0	32.0	36.0
8	34.38711676042376	36.0	36.0	36.0	32.0	36.0
9	34.499332652549356	36.0	36.0	36.0	32.0	36.0
10	34.23891411969854	36.0	36.0	36.0	32.0	36.0
11	34.500457588352624	36.0	36.0	36.0	32.0	36.0
12	34.33831455205609	36.0	36.0	36.0	32.0	36.0
13	34.414817651414715	36.0	36.0	36.0	32.0	36.0
14	34.3262791572611	36.0	36.0	36.0	32.0	36.0
15	34.28395111493305	36.0	36.0	36.0	32.0	36.0
16	34.2714320052074	36.0	36.0	36.0	32.0	36.0
17	34.20220329165024	36.0	36.0	36.0	32.0	36.0
18	34.201908434555165	36.0	36.0	36.0	32.0	36.0
19	34.19619343222617	36.0	36.0	36.0	32.0	36.0
20	34.16407789601921	36.0	36.0	36.0	32.0	36.0
21	34.15083321389755	36.0	36.0	36.0	32.0	36.0
22	34.1275521934382	36.0	36.0	36.0	32.0	36.0
23	34.10050148100374	36.0	36.0	36.0	32.0	36.0
24	34.079954793555245	36.0	36.0	36.0	32.0	36.0
25	33.70001612383102	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	13.0
5	22.0
6	62.0
7	14.0
8	62.0
9	84.0
10	97.0
11	43.0
12	75.0
13	91.0
14	135.0
15	228.0
16	413.0
17	501.0
18	671.0
19	881.0
20	1378.0
21	2007.0
22	3135.0
23	4821.0
24	7036.0
25	10209.0
26	14295.0
27	19033.0
28	25594.0
29	34918.0
30	46206.0
31	62394.0
32	88592.0
33	125308.0
34	270822.0
35	620488.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.47102193340448	17.29200006870291	11.483691646398063	25.75328635149455
2	15.727250399940877	20.025485733140336	39.566787327006445	24.68047653991235
3	19.131385744305224	24.742144139503807	28.615076832349835	27.511393283841134
4	12.220414013452077	15.920332644057423	36.75649648767888	35.10275685481162
5	13.830289167168097	37.42413225213549	34.0899826950613	14.655595885635108
6	32.637125619752034	36.27720992319965	17.773230295786245	13.312434161262074
7	29.249664945663334	30.82522296512822	21.284070079554407	18.641042009654043
8	27.15210375319786	34.24996527720736	19.727474615025038	18.870456354569743
9	27.558188338583467	14.371843759661958	18.780236801108877	39.289731100645696
10	15.535643330674386	27.21118311180423	32.46312578610715	24.79004777141423
11	36.26864099865787	21.138427550027373	23.80394005005538	18.788991401259374
12	25.254631773623466	23.579843381665317	29.93346260371259	21.23206224099863
13	29.75933312418769	20.19487891961338	25.517710154020822	24.528077802178103
14	23.100711266879703	20.389575854274696	26.213941786966533	30.29577109187907
15	24.22230952857557	28.708893396950785	22.967712251537904	24.101084822935746
16	24.24107969858315	26.160714819263433	25.675035705108666	23.923169777044752
17	22.848457007981057	26.576286884847946	26.423985748378204	24.151270358792797
18	23.39672284965957	25.551881739307124	28.212045103772443	22.839350307260858
19	25.24205555045087	24.837913731782805	26.26249496690295	23.657535750863374
20	25.438620738085614	25.062473339105658	26.158194074128303	23.34071184868042
21	26.03427191783943	24.479493100122735	25.197606239733105	24.28862874230473
22	25.203627380010563	25.393975931516355	25.7899875765447	23.612409111928383
23	23.74810987526671	25.419039906285672	26.37934280437732	24.453507414070298
24	24.349439046655718	25.39447518104547	26.540436600842366	23.715649171456445
25	24.161674477118105	25.367569332230683	26.66848947886248	23.80226671178874
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	195.0
1	195.0
2	537.0
3	879.0
4	879.0
5	879.0
6	2424.0
7	3969.0
8	3969.0
9	3969.0
10	3812.5
11	3656.0
12	3656.0
13	3656.0
14	3609.5
15	3563.0
16	3563.0
17	3563.0
18	6532.5
19	9502.0
20	9502.0
21	9502.0
22	16379.5
23	23257.0
24	23257.0
25	23257.0
26	35567.0
27	47877.0
28	47877.0
29	47877.0
30	61544.0
31	75211.0
32	75211.0
33	75211.0
34	86228.5
35	97246.0
36	97246.0
37	97246.0
38	108098.5
39	118951.0
40	118951.0
41	118951.0
42	132536.0
43	146121.0
44	146121.0
45	146121.0
46	160831.0
47	175541.0
48	175541.0
49	175541.0
50	176915.5
51	178290.0
52	178290.0
53	178290.0
54	161845.5
55	145401.0
56	145401.0
57	145401.0
58	131120.0
59	116839.0
60	116839.0
61	116839.0
62	101075.5
63	85312.0
64	85312.0
65	85312.0
66	69290.0
67	53268.0
68	53268.0
69	53268.0
70	40024.0
71	26780.0
72	26780.0
73	26780.0
74	20140.5
75	13501.0
76	13501.0
77	13501.0
78	10719.5
79	7938.0
80	7938.0
81	7938.0
82	5494.0
83	3050.0
84	3050.0
85	3050.0
86	2327.5
87	1605.0
88	1605.0
89	1605.0
90	1157.5
91	710.0
92	710.0
93	710.0
94	495.0
95	280.0
96	280.0
97	280.0
98	485.0
99	690.0
100	690.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03978704599472094
2	0.004404194584781492
3	0.002015478877781361
4	0.004628136682312755
5	0.009704157559688033
6	0.013809762681094509
7	0.022170267655594968
8	0.03388990409306436
9	0.04613207209144004
10	0.0564334085778781
11	0.05337286657828419
12	0.06240519784537843
13	0.0635995556988785
14	0.06673474506431616
15	0.05941930321162826
16	0.06628686086925364
17	0.062479845211222185
18	0.07688678681906673
19	0.07412483428284783
20	0.07920085516022311
21	0.0735276553560978
22	0.0772600236482855
23	0.0818135129647545
24	0.07599101842894168
25	0.07770790784334802
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1339632.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.7189052271122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.42810316181972	39.23768116821704
2	14.850452463390729	15.657991919944617
3	4.867087584803613	7.697625873459484
4	2.1222432819915253	4.475293698087471
5	1.0620571855934904	2.7995246056538363
6	0.6181545269204253	1.955305795225697
7	0.4255085018696263	1.5702639668376726
8	0.27145353288853563	1.1448586459132397
9	0.20230893290839816	0.959895491453644
>10	0.9910252739047538	9.353326012402915
>50	0.07837023462705209	2.896598217088538
>100	0.0744138225517531	7.772360079112827
>500	0.006829545855815559	2.475017453945151
>1k	0.0019919508746128714	2.0042570726577296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4360	0.3254625150787679	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3216	0.2400659285535132	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3199	0.23879692333416938	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2032	0.15168344739450834	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1732	0.12928923764138212	No Hit
GTACATGGGGTGGTATCAACGCAAA	1652	0.12331744837388177	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1595	0.1190625485207878	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1557	0.11622594861872514	No Hit
GTCCTACAGTGGACATTTCTAAATT	1404	0.10480490164463076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.46473658437541E-5
2	0.0	0.0	0.0	0.0	7.46473658437541E-5
3	0.0	0.0	0.0	0.0	7.46473658437541E-5
4	0.0	0.0	0.0	0.0	7.46473658437541E-5
5	0.0	0.0	0.0	0.0	7.46473658437541E-5
6	0.0	0.0	0.0	0.0	7.46473658437541E-5
7	0.0	0.0	0.0	0.0	7.46473658437541E-5
8	0.0	0.0	0.0	0.0	7.46473658437541E-5
9	0.0	0.0	0.0	0.0	7.46473658437541E-5
10	0.0	0.0	0.0	0.0	7.46473658437541E-5
11	0.0	0.0	0.0	0.0	7.46473658437541E-5
12	0.0	0.0	0.0	0.0	1.492947316875082E-4
13	0.0	0.0	0.0	0.0	2.985894633750164E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	65	1.9103936E-7	16.078629	16
CGTCTTA	50	8.7046195E-5	15.201614	15
ACGAACG	70	4.429312E-7	14.930156	15
AATCGTA	45	6.761765E-4	14.777691	13
GCGTTAT	80	1.2868622E-7	14.250979	1
CGCGGTC	85	2.695815E-7	13.412687	10
TAGACTG	80	2.0023017E-6	13.060472	5
CGAGGTT	80	2.004821E-6	13.059009	4
AACGAAC	95	7.3805495E-8	13.000408	14
GTCTAGA	140	3.6379788E-12	12.893744	1
CGTAAAT	60	4.0877078E-4	12.668011	16
GGCTTAG	60	4.088996E-4	12.667538	1
AAGACGG	135	2.7284841E-11	12.6647005	5
ATTTAGA	285	0.0	12.334182	1
CGATAAC	85	3.9391307E-6	12.294963	10
AACCGCG	85	3.942423E-6	12.294045	7
ACACCGT	85	3.9457154E-6	12.293127	6
CGAACGT	55	0.003074212	12.087679	4
ACTGTTC	505	0.0	12.039983	8
AGGCCCG	150	1.4551915E-11	12.034162	10
>>END_MODULE