##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064067_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1339632
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.290351380080498	32.0	32.0	32.0	32.0	32.0
2	31.41216990934824	32.0	32.0	32.0	32.0	32.0
3	31.498616037837255	32.0	32.0	32.0	32.0	32.0
4	31.573266389575643	32.0	32.0	32.0	32.0	32.0
5	31.522065761343413	32.0	32.0	32.0	32.0	32.0
6	35.1207846632508	36.0	36.0	36.0	36.0	36.0
7	35.139907078959	36.0	36.0	36.0	36.0	36.0
8	35.08525624947747	36.0	36.0	36.0	36.0	36.0
9	35.19175788574773	36.0	36.0	36.0	36.0	36.0
10	35.054142480920135	36.0	36.0	36.0	36.0	36.0
11	35.19682644188852	36.0	36.0	36.0	36.0	36.0
12	35.115964682838275	36.0	36.0	36.0	36.0	36.0
13	35.14883042507196	36.0	36.0	36.0	36.0	36.0
14	35.09740436179488	36.0	36.0	36.0	36.0	36.0
15	35.071842864309005	36.0	36.0	36.0	36.0	36.0
16	35.07664567582739	36.0	36.0	36.0	36.0	36.0
17	35.04538709138032	36.0	36.0	36.0	36.0	36.0
18	35.04198466444516	36.0	36.0	36.0	36.0	36.0
19	35.03798431210959	36.0	36.0	36.0	36.0	36.0
20	35.02187167819222	36.0	36.0	36.0	36.0	36.0
21	35.018576743463875	36.0	36.0	36.0	36.0	36.0
22	35.00691607844542	36.0	36.0	36.0	36.0	36.0
23	34.967538100015524	36.0	36.0	36.0	36.0	36.0
24	34.93909521420808	36.0	36.0	36.0	36.0	36.0
25	34.91534092944928	36.0	36.0	36.0	32.0	36.0
26	34.85966220574009	36.0	36.0	36.0	32.0	36.0
27	34.850493269793496	36.0	36.0	36.0	32.0	36.0
28	34.82537592413439	36.0	36.0	36.0	32.0	36.0
29	34.797271190894214	36.0	36.0	36.0	32.0	36.0
30	34.76864317961948	36.0	36.0	36.0	32.0	36.0
31	34.75023364625509	36.0	36.0	36.0	32.0	36.0
32	34.70983822422874	36.0	36.0	36.0	32.0	36.0
33	34.686361627670884	36.0	36.0	36.0	32.0	36.0
34	34.67013702270474	36.0	36.0	36.0	32.0	36.0
35	34.621849134687736	36.0	36.0	36.0	32.0	36.0
36	34.58895129408673	36.0	36.0	36.0	32.0	36.0
37	34.57305812342494	36.0	36.0	36.0	32.0	36.0
38	34.520053268360265	36.0	36.0	36.0	32.0	36.0
39	34.49380800100326	36.0	36.0	36.0	32.0	36.0
40	34.486930739188075	36.0	36.0	36.0	32.0	36.0
41	34.45683665364817	36.0	36.0	36.0	32.0	36.0
42	34.39090436776667	36.0	36.0	36.0	32.0	36.0
43	34.38335602613255	36.0	36.0	36.0	32.0	36.0
44	34.30321834653099	36.0	36.0	36.0	32.0	36.0
45	34.27129241463327	36.0	36.0	36.0	32.0	36.0
46	34.21487617494954	36.0	36.0	36.0	32.0	36.0
47	34.17199872800889	36.0	36.0	36.0	32.0	36.0
48	34.10943975658987	36.0	36.0	36.0	32.0	36.0
49	34.07968904893284	36.0	36.0	36.0	32.0	36.0
50	33.502964993371315	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	12.0
21	36.0
22	110.0
23	396.0
24	1011.0
25	2434.0
26	4994.0
27	9370.0
28	15342.0
29	23528.0
30	33819.0
31	47255.0
32	69435.0
33	111839.0
34	231464.0
35	788584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62362897422039	18.129343012509068	12.025908019959568	26.221119993310975
2	15.08117913155125	20.77693094361876	38.962922598030794	25.17896732679919
3	18.39816725240141	25.295529803796278	28.892199924158053	27.41410301964426
4	11.916630835931063	16.53088310819688	36.83466802823462	34.71781802763744
5	13.485569171235085	37.90548449126327	33.72978549332951	14.879160844172132
6	32.875146775711784	36.58916013314134	17.3919794211681	13.143713669978776
7	29.117570435440804	30.986368634347816	21.275485562815433	18.620575367395947
8	27.165072198932243	34.36264586095285	19.59941237593608	18.872869564178817
9	27.622264605875568	14.11084528017378	18.70231372446561	39.56457638948504
10	15.397087376466557	27.456728303686802	32.54355535947961	24.602628960367028
11	36.48419864559819	21.028685489746437	23.796161931037776	18.69095393361759
12	25.19695736882572	23.603606966102582	29.940729903032924	21.258705762038772
13	29.77621315847817	20.229721346975975	25.662667780412846	24.331397714133008
14	23.0900150862439	20.319699977083243	26.216398396274794	30.373886540398065
15	24.190295036735886	28.75596650248365	22.982661496817617	24.07107696396285
16	24.30607187112763	26.076573953807795	25.649064660127497	23.968289514937073
17	22.869414884087572	26.54661877291674	26.406878904057233	24.177087438938454
18	23.641751826997005	25.419033613759023	28.171584691295358	22.767629867948614
19	25.263449412562117	24.844005550782267	26.337252571790295	23.555292464865325
20	25.575664831576	24.901595595782403	26.258467854810114	23.264271717831484
21	26.05872358976196	24.388414131642122	25.362338313805584	24.19052396479033
22	25.250703552473446	25.33684674126438	25.8887155408583	23.52373416540388
23	23.807195494828367	25.27993096529155	26.39114492511287	24.521728614767216
24	24.467704719910007	25.347877723807372	26.562099934085932	23.622317622196693
25	24.243458008457655	25.195149352612507	26.752984999421475	23.808407639508367
26	23.399922362566812	26.129997909761414	27.115844008480394	23.354235719191376
27	24.00301596070293	25.771533511503947	26.33978828553085	23.885662242262267
28	23.41359303894987	25.759573235550565	26.60424853385569	24.222585191643873
29	23.758655594671826	25.40922785675681	26.206856395510286	24.62526015306107
30	23.499424449046195	25.760421503359993	26.647262661747735	24.092891385846073
31	24.427096825246338	25.333081505027483	25.94179929333792	24.29802237638826
32	23.77467282680181	25.99880111766291	25.862340537824885	24.36418551771039
33	23.436379068758786	25.74085506734639	26.706166992767066	24.11659887112776
34	24.229039373840603	25.689406126478527	26.624464856915708	23.457089642765165
35	24.380852563183794	25.858176866848215	26.37259029668912	23.38838027327887
36	23.579631348033526	26.203165440987092	26.307306523412333	23.90989668756705
37	24.198928022215917	26.045924842114694	25.707535197599245	24.04761193807014
38	23.961870037416276	26.36906841534754	25.82708484506437	23.841976702171817
39	24.53167947422091	25.52498032956746	25.81604301068847	24.127297185523158
40	25.05718124813377	25.86548223350254	26.01216781128695	23.06516870707674
41	24.129431226410585	25.711190936887867	26.666487506569204	23.49289033013234
42	24.707899624367727	26.760216421520326	25.76529850523437	22.76658544887758
43	23.729148844368602	26.125809374939347	26.28742309351798	23.857618687174067
44	23.518043257236787	26.46672847551608	25.69359531924868	24.321632947998452
45	23.672626321364508	26.631154081869546	25.698052953862256	23.99816664290369
46	23.917457435817813	25.802986509647486	25.54545155954208	24.734104494992625
47	24.60572156294533	25.866098735517124	25.59243475099638	23.935744950541167
48	24.42005076142132	26.29501343684682	25.042102120035832	24.24283368169603
49	24.42782623235704	26.22578805459426	25.266641186956377	24.079744526092316
50	24.075012708894285	26.930762839715648	24.882783311299605	24.11144114009046
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	27.0
2	26.0
3	75.0
4	124.0
5	173.0
6	222.0
7	235.0
8	248.0
9	356.5
10	465.0
11	767.5
12	1070.0
13	1934.5
14	2799.0
15	3877.0
16	4955.0
17	5526.5
18	6098.0
19	6010.5
20	5923.0
21	6285.0
22	6647.0
23	7439.5
24	8232.0
25	8945.0
26	9658.0
27	12538.0
28	15418.0
29	18822.0
30	22226.0
31	26230.0
32	30234.0
33	36320.5
34	42407.0
35	49028.0
36	55649.0
37	59884.5
38	64120.0
39	65791.0
40	67462.0
41	69784.5
42	72107.0
43	72999.0
44	73891.0
45	79376.5
46	84862.0
47	88884.0
48	92906.0
49	95072.5
50	97239.0
51	94763.5
52	92288.0
53	90806.5
54	89325.0
55	88332.0
56	87339.0
57	84193.0
58	81047.0
59	73086.0
60	65125.0
61	57345.5
62	49566.0
63	42678.5
64	35791.0
65	29956.5
66	24122.0
67	20431.0
68	16740.0
69	14800.0
70	12860.0
71	9981.5
72	7103.0
73	6114.0
74	5125.0
75	4022.5
76	2920.0
77	2519.0
78	2118.0
79	1711.5
80	1305.0
81	1044.0
82	783.0
83	640.5
84	498.0
85	379.0
86	260.0
87	186.5
88	113.0
89	88.5
90	64.0
91	50.0
92	36.0
93	31.0
94	26.0
95	32.0
96	38.0
97	25.0
98	12.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00925627336462551
2	1.492947316875082E-4
3	2.985894633750164E-4
4	0.0
5	0.0
6	2.239420975312623E-4
7	7.46473658437541E-5
8	0.0
9	0.0012690052193438197
10	0.0011197104876563115
11	0.0
12	1.492947316875082E-4
13	6.718262925937869E-4
14	7.46473658437541E-5
15	0.0052253156090627875
16	8.957683901250491E-4
17	0.0
18	1.492947316875082E-4
19	7.46473658437541E-5
20	5.225315609062787E-4
21	0.0
22	1.492947316875082E-4
23	0.0011943578535000656
24	6.718262925937869E-4
25	0.0012690052193438197
26	0.005374610340750295
27	0.006867557657625377
28	0.004777431414000262
29	0.0026873051703751477
30	0.0034337788288126884
31	0.00709149975515664
32	0.003807015658031459
33	0.004852078779844017
34	0.002761952536218902
35	0.0035830735605001966
36	0.007688678681906673
37	0.003881663023875213
38	0.008061915511125443
39	0.004329547218937738
40	0.002388715707000131
41	0.0035830735605001966
42	0.0017915367802500983
43	0.0013436525851875738
44	0.0013436525851875738
45	9.704157559688033E-4
46	5.971789267500328E-4
47	0.0024633630728438855
48	0.002388715707000131
49	2.985894633750164E-4
50	0.001418299951031328
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1339632.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.81760052927738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.32049917936031	42.79530034038658
2	14.487784315917832	16.463222836322988
3	4.762499189246578	8.117813293668585
4	2.056264227658012	4.67327997878864
5	0.9904443214829256	2.813733490225402
6	0.602979755588534	2.0555917728162383
7	0.38086743113490995	1.5147981489784765
8	0.23993722531847886	1.090612593619884
9	0.1792359150167072	0.9165379157926898
>10	0.8546878582801151	8.564520150787446
>50	0.06041990382845913	2.399550232360553
>100	0.058563980169984	6.287011912273052
>500	0.005552301679133359	2.115140904870636
>1k	2.6439531805396945E-4	0.19288642910894152
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.46473658437541E-5
2	0.0	0.0	0.0	7.46473658437541E-5	7.46473658437541E-5
3	0.0	0.0	0.0	7.46473658437541E-5	7.46473658437541E-5
4	0.0	0.0	0.0	7.46473658437541E-5	7.46473658437541E-5
5	0.0	0.0	0.0	7.46473658437541E-5	7.46473658437541E-5
6	0.0	0.0	0.0	7.46473658437541E-5	2.2394209753126231E-4
7	0.0	0.0	0.0	7.46473658437541E-5	2.2394209753126231E-4
8	0.0	0.0	0.0	7.46473658437541E-5	2.2394209753126231E-4
9	0.0	0.0	0.0	7.46473658437541E-5	2.2394209753126231E-4
10	0.0	0.0	0.0	7.46473658437541E-5	2.2394209753126231E-4
11	0.0	0.0	0.0	7.46473658437541E-5	2.2394209753126231E-4
12	0.0	0.0	0.0	7.46473658437541E-5	3.732368292187705E-4
13	0.0	0.0	0.0	7.46473658437541E-5	5.971789267500328E-4
14	0.0	0.0	0.0	7.46473658437541E-5	5.971789267500328E-4
15	0.0	0.0	0.0	7.46473658437541E-5	8.211210242812951E-4
16	0.0	0.0	0.0	7.46473658437541E-5	8.211210242812951E-4
17	0.0	0.0	0.0	7.46473658437541E-5	8.211210242812951E-4
18	0.0	0.0	0.0	1.492947316875082E-4	8.957683901250493E-4
19	0.0	0.0	0.0	1.492947316875082E-4	8.957683901250493E-4
20	0.0	0.0	0.0	1.492947316875082E-4	0.0011197104876563115
21	0.0	0.0	0.0	1.492947316875082E-4	0.0012690052193438197
22	0.0	0.0	0.0	2.2394209753126231E-4	0.0013436525851875738
23	0.0	0.0	0.0	2.985894633750164E-4	0.0013436525851875738
24	0.0	0.0	0.0	5.225315609062787E-4	0.0013436525851875738
25	0.0	0.0	0.0	5.971789267500328E-4	0.0013436525851875738
26	0.0	0.0	0.0	9.704157559688034E-4	0.001418299951031328
27	0.0	0.0	0.0	0.0011197104876563115	0.001418299951031328
28	0.0	0.0	0.0	0.0017915367802500985	0.001418299951031328
29	0.0	0.0	0.0	0.0029112472679064102	0.001418299951031328
30	0.0	0.0	0.0	0.004777431414000262	0.001418299951031328
31	0.0	0.0	0.0	0.010450631218125575	0.001418299951031328
32	0.0	0.0	0.0	0.01687030468068843	0.001492947316875082
33	0.0	0.0	0.0	0.023513920240782544	0.001492947316875082
34	0.0	0.0	0.0	0.031351893654376725	0.001492947316875082
35	0.0	0.0	0.0	0.04053351965315848	0.001492947316875082
36	0.0	0.0	0.0	0.05389539813919046	0.001492947316875082
37	0.0	0.0	0.0	0.07830508677009805	0.001492947316875082
38	0.0	0.0	0.0	0.1118964013997874	0.001492947316875082
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAC	50	0.0025804162	22.000242	28
TTACGGT	50	0.0025804162	22.000242	21
GTATTAC	180	0.0	21.999422	1
TAGGACG	995	0.0	20.560738	4
TTAGGAC	660	0.0	20.331282	3
TGTAGGA	1205	0.0	19.350744	2
AGGACGT	1035	0.0	19.128504	5
CTGTAGG	1235	0.0	18.882095	1
GCGAGAA	770	0.0	18.856646	20
GGACGTG	1030	0.0	18.367077	6
CCGATTA	60	0.0074120387	18.333534	9
TCGAACT	120	3.1647141E-7	18.332167	19
GTAGGAC	1200	0.0	18.148167	3
CGTCTTA	170	1.2732926E-10	18.118525	15
TACGGAA	85	1.430858E-4	18.117847	37
GTATTAT	135	5.5284545E-8	17.925455	1
ACGGAAA	765	0.0	17.830261	38
TATGGCG	1420	0.0	17.660849	16
GATATAC	500	0.0	17.599537	1
GTCCTAT	440	0.0	17.499538	1
>>END_MODULE