FastQCFastQC Report
Thu 2 Feb 2017
SRR4064066_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064066_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119390
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87270.27976623634749104No Hit
TATCAACGCAGAGTACTTTTTTTTT63270.20282811703570247No Hit
GTACTTTTTTTTTTTTTTTTTTTTT48250.15467767736640817No Hit
GGTATCAACGCAGAGTACTTTTTTT31830.10203918073725952No Hit
GATTAAGAGGGACGGCCGGGGGCAT31430.10075687874872973No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG31330.10043630325159726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2750.012.0924518
CCAACGA2500.011.78169119
AACGGAC658.018816E-411.69379815
GTATAAT2200.011.6570241
CGAACGA1604.5474735E-1111.28268916
CCGAACC600.00588285611.082084
ACTCTAA5750.011.07026610
ATCCCCG4550.011.06663512
GTATAGG2500.011.0180461
GCGTAAC1658.0035534E-1110.93938411
ATTTAGA4800.010.8834871
ACGGTAT3750.010.6410169
AACCGCG2500.010.6401627
CGGTCCA5750.010.57458210
CGCGCTA1355.6834324E-810.55690216
ACGCGCG1355.6888894E-810.55622513
AATCCCC4700.010.5106311
CCGTCGT5250.010.496249
GTATCAA56250.010.367941
CGCCCCT3300.010.3627975
Produced by FastQC (version 0.11.5)